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Determination of phosphate-activated glutaminase activity and its kinetics in mouse tissues using metabolic mapping (quantitative enzyme histochemistry).

Botman D, Tigchelaar W, Van Noorden CJ - J. Histochem. Cytochem. (2014)

Bottom Line: PAG activity was decreased to 22% in the presence of 2 mM 6-diazo-5-oxo-L-norleucine.When compared with liver, kidney and brain, other tissues showed 3-fold to 6-fold less PAG activity.In conclusion, PAG is mainly active in mouse kidney, brain and liver, and shows different kinetics depending on which type of PAG is expressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (DB, WT, CJFVN).

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Phosphate-activated glutaminase (PAG) activity in the presence of 37.5 mM or 30 mM glutamine (for liver, kidney and brain tissue) in various mouse tissues. All test reactions were corrected for nonspecific staining in the absence of substrate. Activity is presented as mean reaction velocity ± SEM (n=3).
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fig11-0022155414551177: Phosphate-activated glutaminase (PAG) activity in the presence of 37.5 mM or 30 mM glutamine (for liver, kidney and brain tissue) in various mouse tissues. All test reactions were corrected for nonspecific staining in the absence of substrate. Activity is presented as mean reaction velocity ± SEM (n=3).

Mentions: Various mouse tissues were metabolically mapped for PAG activity. PAG was mainly active in the liver, kidney and brain (Fig. 11). PAG was twice more active in the kidney and cerebrum than in the liver. Other tissues showed low PAG activity (at least 3-fold less active as compared with liver tissue, and at least 6-fold less as compared with kidney and cerebrum).


Determination of phosphate-activated glutaminase activity and its kinetics in mouse tissues using metabolic mapping (quantitative enzyme histochemistry).

Botman D, Tigchelaar W, Van Noorden CJ - J. Histochem. Cytochem. (2014)

Phosphate-activated glutaminase (PAG) activity in the presence of 37.5 mM or 30 mM glutamine (for liver, kidney and brain tissue) in various mouse tissues. All test reactions were corrected for nonspecific staining in the absence of substrate. Activity is presented as mean reaction velocity ± SEM (n=3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4230542&req=5

fig11-0022155414551177: Phosphate-activated glutaminase (PAG) activity in the presence of 37.5 mM or 30 mM glutamine (for liver, kidney and brain tissue) in various mouse tissues. All test reactions were corrected for nonspecific staining in the absence of substrate. Activity is presented as mean reaction velocity ± SEM (n=3).
Mentions: Various mouse tissues were metabolically mapped for PAG activity. PAG was mainly active in the liver, kidney and brain (Fig. 11). PAG was twice more active in the kidney and cerebrum than in the liver. Other tissues showed low PAG activity (at least 3-fold less active as compared with liver tissue, and at least 6-fold less as compared with kidney and cerebrum).

Bottom Line: PAG activity was decreased to 22% in the presence of 2 mM 6-diazo-5-oxo-L-norleucine.When compared with liver, kidney and brain, other tissues showed 3-fold to 6-fold less PAG activity.In conclusion, PAG is mainly active in mouse kidney, brain and liver, and shows different kinetics depending on which type of PAG is expressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (DB, WT, CJFVN).

Show MeSH
Related in: MedlinePlus