Determination of phosphate-activated glutaminase activity and its kinetics in mouse tissues using metabolic mapping (quantitative enzyme histochemistry).
Bottom Line: PAG activity was decreased to 22% in the presence of 2 mM 6-diazo-5-oxo-L-norleucine.When compared with liver, kidney and brain, other tissues showed 3-fold to 6-fold less PAG activity.In conclusion, PAG is mainly active in mouse kidney, brain and liver, and shows different kinetics depending on which type of PAG is expressed.
Affiliation: Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (DB, WT, CJFVN).Show MeSH
License 1 - License 2 - License 3
Mentions: In the liver, PAG activity in the presence of low glutamine concentrations was highest in periportal areas as compared to pericentral areas after correcting for nonspecific background staining (e.g., staining in the absence of glutamine, Fig. 10). In the presence of 2 and 5 mM glutamine, PAG activity was approximately 1.5-fold higher in the periportal areas as compared with the pericentral areas. With glutamine concentrations higher than 5 mM, PAG activity zonation did not occur.
Affiliation: Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (DB, WT, CJFVN).