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Using mouse models to study function of transcriptional factors in T cell development.

Li P, Xiao Y, Liu Z, Liu P - Cell Regen (Lond) (2012)

Bottom Line: With the advances of genetic engineering and conditional knockout (CKO) mice, we now understand hematopoiesis is a dynamic stepwise process starting from hematopoietic stem cells (HSCs) which are responsible for replenishing all blood cells.Transcriptional factors play important role in hematopoiesis.Finally, we focused on the key transcriptional factor Bcl11b and its function in regulating T cell specification and commitment.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Regenerative Biology, Guangzchou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China ; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou, China.

ABSTRACT
Laboratory mice have widely been used as tools for basic biological research and models for studying human diseases. With the advances of genetic engineering and conditional knockout (CKO) mice, we now understand hematopoiesis is a dynamic stepwise process starting from hematopoietic stem cells (HSCs) which are responsible for replenishing all blood cells. Transcriptional factors play important role in hematopoiesis. In this review we compile several studies on using genetic modified mice and humanized mice to study function of transcriptional factors in lymphopoiesis, including T lymphocyte and Natural killer (NK) cell development. Finally, we focused on the key transcriptional factor Bcl11b and its function in regulating T cell specification and commitment.

No MeSH data available.


Related in: MedlinePlus

Stages in T cell development. Early T cell precursors (ETPs) differentiate from double negative (DN) to double positive (DP) to single positive (SP) stages. Arrows indicate cell differentiation. Note that ETP and DN2 thymocytes contain non-T-cell options. β- and γδ- selection occurs during the accumulation of the DN3 T cells.
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Fig3: Stages in T cell development. Early T cell precursors (ETPs) differentiate from double negative (DN) to double positive (DP) to single positive (SP) stages. Arrows indicate cell differentiation. Note that ETP and DN2 thymocytes contain non-T-cell options. β- and γδ- selection occurs during the accumulation of the DN3 T cells.

Mentions: T cell development, which happens in the thymus, involves progenitor homing, lineage specification and commitment [67]. It also requires the intrathymic microenvironment and interactions among key transcription factors [5, 68]. In adult mice, CLPs migrate from the bone marrow to the thymus and initiate the program of T cell development [69]. In the thymus, thymocytes are classified into three distinct maturational---double negative (DN; CD4-CD8-), double positive (DP; CD4+CD8+) and single-positive (SP; CD4-CD8+ or CD4+CD8-) (Figure 3) [70].Figure 3


Using mouse models to study function of transcriptional factors in T cell development.

Li P, Xiao Y, Liu Z, Liu P - Cell Regen (Lond) (2012)

Stages in T cell development. Early T cell precursors (ETPs) differentiate from double negative (DN) to double positive (DP) to single positive (SP) stages. Arrows indicate cell differentiation. Note that ETP and DN2 thymocytes contain non-T-cell options. β- and γδ- selection occurs during the accumulation of the DN3 T cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230505&req=5

Fig3: Stages in T cell development. Early T cell precursors (ETPs) differentiate from double negative (DN) to double positive (DP) to single positive (SP) stages. Arrows indicate cell differentiation. Note that ETP and DN2 thymocytes contain non-T-cell options. β- and γδ- selection occurs during the accumulation of the DN3 T cells.
Mentions: T cell development, which happens in the thymus, involves progenitor homing, lineage specification and commitment [67]. It also requires the intrathymic microenvironment and interactions among key transcription factors [5, 68]. In adult mice, CLPs migrate from the bone marrow to the thymus and initiate the program of T cell development [69]. In the thymus, thymocytes are classified into three distinct maturational---double negative (DN; CD4-CD8-), double positive (DP; CD4+CD8+) and single-positive (SP; CD4-CD8+ or CD4+CD8-) (Figure 3) [70].Figure 3

Bottom Line: With the advances of genetic engineering and conditional knockout (CKO) mice, we now understand hematopoiesis is a dynamic stepwise process starting from hematopoietic stem cells (HSCs) which are responsible for replenishing all blood cells.Transcriptional factors play important role in hematopoiesis.Finally, we focused on the key transcriptional factor Bcl11b and its function in regulating T cell specification and commitment.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Regenerative Biology, Guangzchou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China ; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou, China.

ABSTRACT
Laboratory mice have widely been used as tools for basic biological research and models for studying human diseases. With the advances of genetic engineering and conditional knockout (CKO) mice, we now understand hematopoiesis is a dynamic stepwise process starting from hematopoietic stem cells (HSCs) which are responsible for replenishing all blood cells. Transcriptional factors play important role in hematopoiesis. In this review we compile several studies on using genetic modified mice and humanized mice to study function of transcriptional factors in lymphopoiesis, including T lymphocyte and Natural killer (NK) cell development. Finally, we focused on the key transcriptional factor Bcl11b and its function in regulating T cell specification and commitment.

No MeSH data available.


Related in: MedlinePlus