Drivers shaping the diversity and biogeography of total and active bacterial communities in the South China Sea.
Bottom Line: Although the composition of both the total and active bacterial community was strongly correlated with environmental factors and weakly correlated with geographic distance, the active bacterial community displayed higher environmental sensitivity than the total community and particularly a greater distance effect largely caused by the active assemblage from deep waters.This might be due to a high competition between active bacterial taxa as indicated by our community network models.Based on these analyses, we speculate that high competition could cause some dispersal limitation of the active bacterial community resulting in a distinct distance-decay relationship.
Affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiang'an, Xiamen, 361101, China; Institute of Marine Microbes and Ecospheres, Xiamen University, Xiang'an, Xiamen, 361101, China.Show MeSH
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Mentions: A 462-km transect (the P-A transect) from the inner Pearl River estuary to the open waters and a 476-km transect (the S transect) from the Luzon Strait to the SCS basin (Fig.1) were sampled during a winter research cruise (January 2010). In total, 30 samples were collected from the surface and deep waters. The P1 and P2 sites were located in the inner Pearl River estuary with a depth of ∼8.5 m, where the surface water (0.5 m) was sampled. The A3–A8 and S10–S12 sites were sampled from the surface (5 m) and the oxygen minimum layer (OML), which was the near-bottom layer at sites A3–A7 and at ∼800 m for the A8–S12 sites (Fig.1). S9 is the Southeast Asia Time-Series Study station (SEATS) of the SCS central basin with a depth of 3850 m, at which samples were collected from the vertical profile at ten depths from the surface to 3500 m. Two litre samples for DNA or RNA analyses were filtered through 0.2-μm-pore-size polycarbonate filters (Millipore) at a pressure of <0.03 MPa on board. Samples for RNA extraction were collected within 30 min and stored in 2-mL RNase-free tubes with RNAlater RNA stabilization solution (Ambion). All filters were flash-frozen in liquid nitrogen for 10 min and subsequently stored at −80 °C until DNA or RNA extraction.
Affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiang'an, Xiamen, 361101, China; Institute of Marine Microbes and Ecospheres, Xiamen University, Xiang'an, Xiamen, 361101, China.