Quantitative biocompatibility evaluation of nickel-free high-nitrogen stainless steel in vitro/in vivo.
Bottom Line: Endothelialization was greater with HNS than with commercial stainless steel (SUS316L).These differences may be related to the amounts of nickel ion eluted from the stents, as HNS did not elute nickel ion.These data suggest that HNS may be useful as a material for coronary artery stents.
Affiliation: Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA) National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan.Show MeSH
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Mentions: Surface morphology of biomaterials and protein adsorption on biomaterials affect cell adhesion and proliferation. However, in our experiment, the surface morphology of metals was not supposed to affect cell adhesion and proliferation, because mirror-polished metals were used. To compare the cell adhesion properties, we incubated each metal in human FN-containing PBS and assessed the level of FN adsorbed, as FN is a common cell adhesion molecule that is present in the extracellular matrix and in the serum.30 Furthermore, the adsorption of FN is a key mediator of initial cell adhesion. Figure 2 shows the amount of FN adsorbed on the surface. The amount of FN was 1.09-fold higher for HNS than for SUS316L, but this difference did not reach the level of statistical significance. This suggests that factors other than surface morphology and adsorption of cell adhesion protein resulted in the decreased number of HUVECs on SUS316L when compared with HNS.
Affiliation: Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA) National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan.