Identification of a transcriptional signature for the wound healing continuum.
Bottom Line: Central to these outcomes is the role of the fibroblast.Genes whose expression increases following serum exposure in the order OMF < NF < CWF are candidates for a negative/impaired healing phenotype (the dysfunctional healing group), whereas genes with the converse pattern are potentially associated with a positive/preferential healing phenotype (the enhanced healing group).Sixty-six genes in the enhanced healing group and 38 genes in the dysfunctional healing group were identified.
Affiliation: Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Tissue Engineering and Reparative Dentistry, School of Dentistry.Show MeSH
Related in: MedlinePlus
Mentions: To illustrate the concept of a continuum of cellular responses between OMF, NF, and CWF, the growth kinetics of these cells were investigated through senescence. OMF had the greatest proliferative potential, proliferating for much longer in culture and senescing later than patient-matched NF1 (mean of 101.5 PD vs. 56.8 PD, respectively; p < 0.02; Figure 3). Conversely, CWF had the least proliferative potential, growing for a much shorter period of time in culture and senescing earlier than patient-matched NF2 (mean of 18.04 PD vs. 48.03 PD, respectively; p < 0.02). A comparison of NF1 (head/neck) with NF2 (thigh) showed a similar growth potential (p > 0.2). Therefore, for further analysis, three “sets” will be considered: OMF (four patient samples), NF (seven patient samples; 3× head/neck [one of the four samples failed quality control, see above] and 4× thigh), and CWF (four patient samples).
Affiliation: Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Tissue Engineering and Reparative Dentistry, School of Dentistry.