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Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

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Related in: MedlinePlus

Time spent in the respective compartments of the triple test. The behavior of control mice (control), mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and automatically (ABS) were studied in the triple test. The overall time spent in the open field (OF), elevated plus maze (EPM) and light–dark box (LDB) as well as the time spent further subdivided into open-field inner (OF IZ) and outer (OF OZ) zones, elevated plus maze open (EPM OA) and closed (EPM CA) arms, as well as in the light (LDB-L) and dark compartments (LDB-D) of the light–dark box. Data are presented as percentages of the total time spent (15 min). Mice sampled by cheek blood spent significantly less time than mice sampled by tail blood in the OF OZ (P = 0.047), and mice sampled by ABS spent significantly less time in the EPM CA than the control mice (P = 0.014).
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fig5-0023677214541438: Time spent in the respective compartments of the triple test. The behavior of control mice (control), mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and automatically (ABS) were studied in the triple test. The overall time spent in the open field (OF), elevated plus maze (EPM) and light–dark box (LDB) as well as the time spent further subdivided into open-field inner (OF IZ) and outer (OF OZ) zones, elevated plus maze open (EPM OA) and closed (EPM CA) arms, as well as in the light (LDB-L) and dark compartments (LDB-D) of the light–dark box. Data are presented as percentages of the total time spent (15 min). Mice sampled by cheek blood spent significantly less time than mice sampled by tail blood in the OF OZ (P = 0.047), and mice sampled by ABS spent significantly less time in the EPM CA than the control mice (P = 0.014).

Mentions: The was a significant difference between groups regarding the total time spent (Figure 5) in the OF, (F(3) = 3.544, P = 0.023), EPM (F(3) = 3.537, P = 0.023) and LDB (F(3) = 4.134, P = 0.012), where mice sampled by facial vein phlebotomy spent significantly less time in the OF than mice sampled by caudal vein phlebotomy (P = 0.026). Mice sampled by ABS spent significantly less time in the EPM compared with control mice (P = 0.017), and mice sampled by caudal vein phlebotomy spent significantly less time in the LDB than mice sampled by facial vein phlebotomy (P = 0.032) and ABS (P = 0.039).Figure 5.


Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Time spent in the respective compartments of the triple test. The behavior of control mice (control), mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and automatically (ABS) were studied in the triple test. The overall time spent in the open field (OF), elevated plus maze (EPM) and light–dark box (LDB) as well as the time spent further subdivided into open-field inner (OF IZ) and outer (OF OZ) zones, elevated plus maze open (EPM OA) and closed (EPM CA) arms, as well as in the light (LDB-L) and dark compartments (LDB-D) of the light–dark box. Data are presented as percentages of the total time spent (15 min). Mice sampled by cheek blood spent significantly less time than mice sampled by tail blood in the OF OZ (P = 0.047), and mice sampled by ABS spent significantly less time in the EPM CA than the control mice (P = 0.014).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4230456&req=5

fig5-0023677214541438: Time spent in the respective compartments of the triple test. The behavior of control mice (control), mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and automatically (ABS) were studied in the triple test. The overall time spent in the open field (OF), elevated plus maze (EPM) and light–dark box (LDB) as well as the time spent further subdivided into open-field inner (OF IZ) and outer (OF OZ) zones, elevated plus maze open (EPM OA) and closed (EPM CA) arms, as well as in the light (LDB-L) and dark compartments (LDB-D) of the light–dark box. Data are presented as percentages of the total time spent (15 min). Mice sampled by cheek blood spent significantly less time than mice sampled by tail blood in the OF OZ (P = 0.047), and mice sampled by ABS spent significantly less time in the EPM CA than the control mice (P = 0.014).
Mentions: The was a significant difference between groups regarding the total time spent (Figure 5) in the OF, (F(3) = 3.544, P = 0.023), EPM (F(3) = 3.537, P = 0.023) and LDB (F(3) = 4.134, P = 0.012), where mice sampled by facial vein phlebotomy spent significantly less time in the OF than mice sampled by caudal vein phlebotomy (P = 0.026). Mice sampled by ABS spent significantly less time in the EPM compared with control mice (P = 0.017), and mice sampled by caudal vein phlebotomy spent significantly less time in the LDB than mice sampled by facial vein phlebotomy (P = 0.032) and ABS (P = 0.039).Figure 5.

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

Show MeSH
Related in: MedlinePlus