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Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

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Related in: MedlinePlus

Fecal corticosterone metabolite (FCM) levels after 24 h of blood sampling in mice sampled by facial vein phlebotomy (cheek), caudal vein phlebotomy (tail) and automatically (ABS), and in control mice (control) after 24 h of no intervention. Data are presented as mean ± SEM. P values < 0.05 were considered significant (*) and P < 0.01 strongly significant (**).
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fig4-0023677214541438: Fecal corticosterone metabolite (FCM) levels after 24 h of blood sampling in mice sampled by facial vein phlebotomy (cheek), caudal vein phlebotomy (tail) and automatically (ABS), and in control mice (control) after 24 h of no intervention. Data are presented as mean ± SEM. P values < 0.05 were considered significant (*) and P < 0.01 strongly significant (**).

Mentions: The total amount of voided feces (mean ± SD) was 1.24 ± 0.21 g for control mice (Group 1 a), 1.07 ± 0.36 g for ABS mice, 1.08 ± 0.21 g for cheek-bled mice and 1.12 ± 0.21 g for tail-bled mice. FCM levels (Figure 4) were significantly different between groups (F(3) = 6.100, P = 0.001), where mice sampled by caudal vein phlebotomy excreted significantly less FCM compared with mice sampled by facial vein phlebotomy (P = 0.002) and ABS (P = 0.024).Figure 4.


Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Fecal corticosterone metabolite (FCM) levels after 24 h of blood sampling in mice sampled by facial vein phlebotomy (cheek), caudal vein phlebotomy (tail) and automatically (ABS), and in control mice (control) after 24 h of no intervention. Data are presented as mean ± SEM. P values < 0.05 were considered significant (*) and P < 0.01 strongly significant (**).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4230456&req=5

fig4-0023677214541438: Fecal corticosterone metabolite (FCM) levels after 24 h of blood sampling in mice sampled by facial vein phlebotomy (cheek), caudal vein phlebotomy (tail) and automatically (ABS), and in control mice (control) after 24 h of no intervention. Data are presented as mean ± SEM. P values < 0.05 were considered significant (*) and P < 0.01 strongly significant (**).
Mentions: The total amount of voided feces (mean ± SD) was 1.24 ± 0.21 g for control mice (Group 1 a), 1.07 ± 0.36 g for ABS mice, 1.08 ± 0.21 g for cheek-bled mice and 1.12 ± 0.21 g for tail-bled mice. FCM levels (Figure 4) were significantly different between groups (F(3) = 6.100, P = 0.001), where mice sampled by caudal vein phlebotomy excreted significantly less FCM compared with mice sampled by facial vein phlebotomy (P = 0.002) and ABS (P = 0.024).Figure 4.

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

Show MeSH
Related in: MedlinePlus