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Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

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Related in: MedlinePlus

Plasma corticosterone levels during 24 h of blood sampling in mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and automatically (ABS) and from control mice (control). Top: Actual values at 09:00 and 18:00 h, where control mice were sampled. Bottom: data are presented as mean ± SD. The grey areas represent 95% CI of the control mice at 09:00 h (2.89–91.51 ng/mL) and 18:00 h (30.42–118.27 ng/mL), respectively.
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fig3-0023677214541438: Plasma corticosterone levels during 24 h of blood sampling in mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and automatically (ABS) and from control mice (control). Top: Actual values at 09:00 and 18:00 h, where control mice were sampled. Bottom: data are presented as mean ± SD. The grey areas represent 95% CI of the control mice at 09:00 h (2.89–91.51 ng/mL) and 18:00 h (30.42–118.27 ng/mL), respectively.

Mentions: Log-transformed plasma corticosterone levels (Figure 3) were significantly different between groups (F(6,44) = 4.934, P = 0.001) at two time points. At 09:00 h, the plasma corticosterone concentrations of mice sampled by facial vein phlebotomy were significantly higher than those of control mice (P = 0.039). At 18:00 h, the plasma corticosterone concentrations of control mice were significantly lower than those of mice sampled by facial vein phlebotomy (P < 0.001) and tail vein phlebotomy (P = 0.001).Figure 3.


Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Plasma corticosterone levels during 24 h of blood sampling in mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and automatically (ABS) and from control mice (control). Top: Actual values at 09:00 and 18:00 h, where control mice were sampled. Bottom: data are presented as mean ± SD. The grey areas represent 95% CI of the control mice at 09:00 h (2.89–91.51 ng/mL) and 18:00 h (30.42–118.27 ng/mL), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4230456&req=5

fig3-0023677214541438: Plasma corticosterone levels during 24 h of blood sampling in mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and automatically (ABS) and from control mice (control). Top: Actual values at 09:00 and 18:00 h, where control mice were sampled. Bottom: data are presented as mean ± SD. The grey areas represent 95% CI of the control mice at 09:00 h (2.89–91.51 ng/mL) and 18:00 h (30.42–118.27 ng/mL), respectively.
Mentions: Log-transformed plasma corticosterone levels (Figure 3) were significantly different between groups (F(6,44) = 4.934, P = 0.001) at two time points. At 09:00 h, the plasma corticosterone concentrations of mice sampled by facial vein phlebotomy were significantly higher than those of control mice (P = 0.039). At 18:00 h, the plasma corticosterone concentrations of control mice were significantly lower than those of mice sampled by facial vein phlebotomy (P < 0.001) and tail vein phlebotomy (P = 0.001).Figure 3.

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

Show MeSH
Related in: MedlinePlus