Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.
Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.
Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark firstname.lastname@example.org.Show MeSH
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Mentions: Log-transformed plasma corticosterone levels (Figure 3) were significantly different between groups (F(6,44) = 4.934, P = 0.001) at two time points. At 09:00 h, the plasma corticosterone concentrations of mice sampled by facial vein phlebotomy were significantly higher than those of control mice (P = 0.039). At 18:00 h, the plasma corticosterone concentrations of control mice were significantly lower than those of mice sampled by facial vein phlebotomy (P < 0.001) and tail vein phlebotomy (P = 0.001).Figure 3.
Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark email@example.com.