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Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

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Related in: MedlinePlus

Daily food intake (DFI) and daily water intake (DWI), as shown from two days prior to surgery for mice sampled automatically (ABS), or two days prior to blood sampling for mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy. Surgery at day 0 and blood sampling at day 3 are indicated by grey lines. Data are presented as mean ± SEM. The grey areas represent 95% CI of DFI (2.53–7.41 g/day) and DWI (4.05–8.37 mL/day) of the control mice.
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fig2-0023677214541438: Daily food intake (DFI) and daily water intake (DWI), as shown from two days prior to surgery for mice sampled automatically (ABS), or two days prior to blood sampling for mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy. Surgery at day 0 and blood sampling at day 3 are indicated by grey lines. Data are presented as mean ± SEM. The grey areas represent 95% CI of DFI (2.53–7.41 g/day) and DWI (4.05–8.37 mL/day) of the control mice.

Mentions: Overall, the DFI (Figure 2) differed significantly between groups (F(3,47) = 6.815, P = 0.001), where mice sampled by caudal vein phlebotomy on average consumed more feed than did mice sampled by facial vein phlebotomy (P = 0.001) and ABS (P = 0.026), although this did not differ significantly from the DFI of control mice. The DFI within the ABS group increased significantly from day 1 to day 3 (P = 0.046).Figure 2.


Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Daily food intake (DFI) and daily water intake (DWI), as shown from two days prior to surgery for mice sampled automatically (ABS), or two days prior to blood sampling for mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy. Surgery at day 0 and blood sampling at day 3 are indicated by grey lines. Data are presented as mean ± SEM. The grey areas represent 95% CI of DFI (2.53–7.41 g/day) and DWI (4.05–8.37 mL/day) of the control mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4230456&req=5

fig2-0023677214541438: Daily food intake (DFI) and daily water intake (DWI), as shown from two days prior to surgery for mice sampled automatically (ABS), or two days prior to blood sampling for mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy. Surgery at day 0 and blood sampling at day 3 are indicated by grey lines. Data are presented as mean ± SEM. The grey areas represent 95% CI of DFI (2.53–7.41 g/day) and DWI (4.05–8.37 mL/day) of the control mice.
Mentions: Overall, the DFI (Figure 2) differed significantly between groups (F(3,47) = 6.815, P = 0.001), where mice sampled by caudal vein phlebotomy on average consumed more feed than did mice sampled by facial vein phlebotomy (P = 0.001) and ABS (P = 0.026), although this did not differ significantly from the DFI of control mice. The DFI within the ABS group increased significantly from day 1 to day 3 (P = 0.046).Figure 2.

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

Show MeSH
Related in: MedlinePlus