Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.
Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.
Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark email@example.com.Show MeSH
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Mentions: The BW (Figure 1) changed significantly between groups (F(3,46) = 21.742, P < 0.001), where mice sampled by ABS lost weight compared with control mice (P < 0.001), mice sampled by facial vein phlebotomy (P < 0.001), and mice sampled by caudal vein phlebotomy (P < 0.001). Furthermore, a significant decrease in BW from days 3 to 4 was found for mice sampled by facial vein phlebotomy (F(1,17) = 17.378, P = 0.001).Figure 1.
Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark firstname.lastname@example.org.