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Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

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Related in: MedlinePlus

Body weight (BW) in relation to baseline values. Data are presented as percentages of the average weight recorded for three days prior to intervention. Sample points represent mean ± SEM for control mice (control), mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and mice sampled automatically (ABS). The mice in the ABS group was catheterized at day 0 and allowed to recover until day 3, where blood sampling was initiated (vertical grey line).
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fig1-0023677214541438: Body weight (BW) in relation to baseline values. Data are presented as percentages of the average weight recorded for three days prior to intervention. Sample points represent mean ± SEM for control mice (control), mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and mice sampled automatically (ABS). The mice in the ABS group was catheterized at day 0 and allowed to recover until day 3, where blood sampling was initiated (vertical grey line).

Mentions: The BW (Figure 1) changed significantly between groups (F(3,46) = 21.742, P < 0.001), where mice sampled by ABS lost weight compared with control mice (P < 0.001), mice sampled by facial vein phlebotomy (P < 0.001), and mice sampled by caudal vein phlebotomy (P < 0.001). Furthermore, a significant decrease in BW from days 3 to 4 was found for mice sampled by facial vein phlebotomy (F(1,17) = 17.378, P = 0.001).Figure 1.


Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.

Teilmann AC, Kalliokoski O, Sørensen DB, Hau J, Abelson KS - Lab. Anim. (2014)

Body weight (BW) in relation to baseline values. Data are presented as percentages of the average weight recorded for three days prior to intervention. Sample points represent mean ± SEM for control mice (control), mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and mice sampled automatically (ABS). The mice in the ABS group was catheterized at day 0 and allowed to recover until day 3, where blood sampling was initiated (vertical grey line).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4230456&req=5

fig1-0023677214541438: Body weight (BW) in relation to baseline values. Data are presented as percentages of the average weight recorded for three days prior to intervention. Sample points represent mean ± SEM for control mice (control), mice sampled by facial vein (cheek) and caudal vein (tail) phlebotomy and mice sampled automatically (ABS). The mice in the ABS group was catheterized at day 0 and allowed to recover until day 3, where blood sampling was initiated (vertical grey line).
Mentions: The BW (Figure 1) changed significantly between groups (F(3,46) = 21.742, P < 0.001), where mice sampled by ABS lost weight compared with control mice (P < 0.001), mice sampled by facial vein phlebotomy (P < 0.001), and mice sampled by caudal vein phlebotomy (P < 0.001). Furthermore, a significant decrease in BW from days 3 to 4 was found for mice sampled by facial vein phlebotomy (F(1,17) = 17.378, P = 0.001).Figure 1.

Bottom Line: The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery.We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling.The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark annete@sund.ku.dk.

Show MeSH
Related in: MedlinePlus