Analysis of flexural rigidity of actin filaments propelled by surface adsorbed myosin motors.
Bottom Line: Actin filaments are central components of the cytoskeleton and the contractile machinery of muscle.The filaments are known to exist in a range of conformational states presumably with different flexural rigidity and thereby different persistence lengths.Our results analyze the approaches proposed previously to measure the persistence length from the statistics of the winding paths of actin filaments that are propelled by surface-adsorbed myosin motor fragments in the in vitro motility assay.
Affiliation: Faculty of Health and Life Sciences, Linnaeus University, Kalmar, Sweden.Show MeSH
Related in: MedlinePlus
Mentions: Monte-Carlo simulations were performed to analyze critical factors in path persistence length measurements. The simulations have appreciable advantages over experimental data for this purpose as an unambiguous theoretical persistence length (LPtheor) can be defined. The similarity between simulated and experimental data based on 500 and 300 independent paths, respectively, are illustrated in Fig. 1. These data were simulated with LPtheor = 10 µm, compared to experimental data with LP ≈ 11.52 ± 0.46 µm (from fit of Eq. 1).
Affiliation: Faculty of Health and Life Sciences, Linnaeus University, Kalmar, Sweden.