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Electrically-driven modulation of surface-grafted RGD peptides for manipulation of cell adhesion.

Lashkor M, Rawson FJ, Stephenson-Brown A, Preece JA, Mendes PM - Chem. Commun. (Camb.) (2014)

Bottom Line: Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine-glycine-aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. p.m.mendes@bham.ac.uk.

ABSTRACT
Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine-glycine-aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

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Related in: MedlinePlus

Microscopic images and density of adhered cells on C3K-GRGDS:C11TEG mixed SAMs that were incubated with cells for 1 h while applying –0.4 V and subsequently in OC conditions for 1 h. The density was normalized against the density of cells adherent onto C3K-GRGDS:C11TEG mixed SAMs that were incubated with cells in OC conditions for 1 h.
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fig4: Microscopic images and density of adhered cells on C3K-GRGDS:C11TEG mixed SAMs that were incubated with cells for 1 h while applying –0.4 V and subsequently in OC conditions for 1 h. The density was normalized against the density of cells adherent onto C3K-GRGDS:C11TEG mixed SAMs that were incubated with cells in OC conditions for 1 h.

Mentions: In contrast, a reversal of the switching sequence demonstrated that our surfaces can be dynamically switched from a non-adhesive to cell-adhesive state. Cells were incubated in the C3K-GRGDS:C11TEG mixed SAMs for 1 h while holding the potential at –0.4 V for 1 h making the RGD peptide inaccessible for recognition by the corresponding integrin. As above, the number of adherent cells when a negative potential of –0.4 V was applied was 70% of the number that adhered to the C3K-GRGDS:C11TEG mixed SAMs under OC conditions, Fig. 4. The potential was then shifted to open circuit conditions for 1 h on those exposed to a potential of –0.4 V, which resulted in a significant increase in the number of cells as a result of the exposure of the RGD moiety to the cells (Fig. 4). These values were similar to those obtained for the samples that were only incubated for 1 hour under OC conditions (Fig. 4), indicating that the surfaces were highly effective at switching from a non-adhesive to cell-adhesive state.


Electrically-driven modulation of surface-grafted RGD peptides for manipulation of cell adhesion.

Lashkor M, Rawson FJ, Stephenson-Brown A, Preece JA, Mendes PM - Chem. Commun. (Camb.) (2014)

Microscopic images and density of adhered cells on C3K-GRGDS:C11TEG mixed SAMs that were incubated with cells for 1 h while applying –0.4 V and subsequently in OC conditions for 1 h. The density was normalized against the density of cells adherent onto C3K-GRGDS:C11TEG mixed SAMs that were incubated with cells in OC conditions for 1 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230383&req=5

fig4: Microscopic images and density of adhered cells on C3K-GRGDS:C11TEG mixed SAMs that were incubated with cells for 1 h while applying –0.4 V and subsequently in OC conditions for 1 h. The density was normalized against the density of cells adherent onto C3K-GRGDS:C11TEG mixed SAMs that were incubated with cells in OC conditions for 1 h.
Mentions: In contrast, a reversal of the switching sequence demonstrated that our surfaces can be dynamically switched from a non-adhesive to cell-adhesive state. Cells were incubated in the C3K-GRGDS:C11TEG mixed SAMs for 1 h while holding the potential at –0.4 V for 1 h making the RGD peptide inaccessible for recognition by the corresponding integrin. As above, the number of adherent cells when a negative potential of –0.4 V was applied was 70% of the number that adhered to the C3K-GRGDS:C11TEG mixed SAMs under OC conditions, Fig. 4. The potential was then shifted to open circuit conditions for 1 h on those exposed to a potential of –0.4 V, which resulted in a significant increase in the number of cells as a result of the exposure of the RGD moiety to the cells (Fig. 4). These values were similar to those obtained for the samples that were only incubated for 1 hour under OC conditions (Fig. 4), indicating that the surfaces were highly effective at switching from a non-adhesive to cell-adhesive state.

Bottom Line: Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine-glycine-aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. p.m.mendes@bham.ac.uk.

ABSTRACT
Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine-glycine-aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

Show MeSH
Related in: MedlinePlus