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Electrically-driven modulation of surface-grafted RGD peptides for manipulation of cell adhesion.

Lashkor M, Rawson FJ, Stephenson-Brown A, Preece JA, Mendes PM - Chem. Commun. (Camb.) (2014)

Bottom Line: Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine-glycine-aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. p.m.mendes@bham.ac.uk.

ABSTRACT
Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine-glycine-aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

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Related in: MedlinePlus

Microscopic images and density of adhered cells on C3K-GRGDS:C11TEG mixed SAM, pure C11TEG SAM and bare gold surfaces that were normalized against the density of cells adherent onto the C3K-GRGDS:C11TEG mixed SAM. The surfaces were cultured in RAW 264.7 mouse macrophage cells under OC conditions for 24 hours.
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fig2: Microscopic images and density of adhered cells on C3K-GRGDS:C11TEG mixed SAM, pure C11TEG SAM and bare gold surfaces that were normalized against the density of cells adherent onto the C3K-GRGDS:C11TEG mixed SAM. The surfaces were cultured in RAW 264.7 mouse macrophage cells under OC conditions for 24 hours.

Mentions: The C3K-GRGDS:C11TEG mixed SAMs were shown to support adhesion of immune macrophage cells as determined by cell counting42,43 (Fig. 2). When RAW 264.7 mouse macrophages were cultured on the C3K-GRGDS:C11TEG mixed SAM in supplemented Dulbecco's Modified Eagle Medium (DMEM), the number of cells adhered to the surface increased with incubation time, reaching 1792 ± 157 cells per mm2 after 24 hours. This is in contrast with the weak cell adhesion observed in two control surfaces, pure C11TEG SAMs and clean gold, in which the number of cells that adhere was 60% and 50% lower, respectively, after 24 hours (Fig. 2).


Electrically-driven modulation of surface-grafted RGD peptides for manipulation of cell adhesion.

Lashkor M, Rawson FJ, Stephenson-Brown A, Preece JA, Mendes PM - Chem. Commun. (Camb.) (2014)

Microscopic images and density of adhered cells on C3K-GRGDS:C11TEG mixed SAM, pure C11TEG SAM and bare gold surfaces that were normalized against the density of cells adherent onto the C3K-GRGDS:C11TEG mixed SAM. The surfaces were cultured in RAW 264.7 mouse macrophage cells under OC conditions for 24 hours.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230383&req=5

fig2: Microscopic images and density of adhered cells on C3K-GRGDS:C11TEG mixed SAM, pure C11TEG SAM and bare gold surfaces that were normalized against the density of cells adherent onto the C3K-GRGDS:C11TEG mixed SAM. The surfaces were cultured in RAW 264.7 mouse macrophage cells under OC conditions for 24 hours.
Mentions: The C3K-GRGDS:C11TEG mixed SAMs were shown to support adhesion of immune macrophage cells as determined by cell counting42,43 (Fig. 2). When RAW 264.7 mouse macrophages were cultured on the C3K-GRGDS:C11TEG mixed SAM in supplemented Dulbecco's Modified Eagle Medium (DMEM), the number of cells adhered to the surface increased with incubation time, reaching 1792 ± 157 cells per mm2 after 24 hours. This is in contrast with the weak cell adhesion observed in two control surfaces, pure C11TEG SAMs and clean gold, in which the number of cells that adhere was 60% and 50% lower, respectively, after 24 hours (Fig. 2).

Bottom Line: Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine-glycine-aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. p.m.mendes@bham.ac.uk.

ABSTRACT
Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine-glycine-aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

Show MeSH
Related in: MedlinePlus