Limits...
Fine-tuned characterization of Staphylococcus aureus Newbould 305, a strain associated with mild and chronic mastitis in bovines.

Peton V, Bouchard DS, Almeida S, Rault L, Falentin H, Jardin J, Jan G, Hernandez D, François P, Schrenzel J, Azevedo V, Miyoshi A, Berkova N, Even S, Le Loir Y - Vet. Res. (2014)

Bottom Line: The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide.In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells.N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. vincent.peton@rennes.inra.fr.

ABSTRACT
S. aureus is a major aetiological agent of ruminant mastitis worldwide. The chronic nature of S. aureus mastitis makes it difficult to cure and prone to resurgence. In order to identify the bacterial factors involved in this chronicity, Newbould 305 (N305), a strain that can reproducibly induce mild and chronic mastitis in an experimental setting, was characterized in depth. We employed genomic and proteomic techniques combined with phenotype characterization, in order to comprehensively analyse N305. The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide. Five mobile genetic elements were identified in the N305 genome as carrying virulence factors which correlated with phenotypic features such as cytotoxicity, mammary epithelial cell invasion or host-adaptation. In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells. N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential. Our data are consistent with the invasiveness and host-adaptation features which contribute to the chronicity of S. aureus mastitis. Mobile genetic elements, exoproteins and surface exposed proteins constitute good targets for further research to explore the underlying mechanisms related to mastitis chronicity.

No MeSH data available.


Related in: MedlinePlus

Comparison of the FnbA found in RF122 and N305 with that ofS. aureus8325.4. The FnbA in S. aureus 8325.4 (SWISS-Prot P14738) contains 11 Fn-binding sites. FnbA from S. aureus Newbould 305 (A) and RF122 (B) showing the approximate positions of the predicted fibronectin binding regions (FnbRs). High-affinity FnbRs are shaded. Signal peptide (S) in red, proline-rich repeats (PRR) in green; cell wall-spanning sequence (W) in white; membrane-spanning region (M) in white.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4230361&req=5

Fig2: Comparison of the FnbA found in RF122 and N305 with that ofS. aureus8325.4. The FnbA in S. aureus 8325.4 (SWISS-Prot P14738) contains 11 Fn-binding sites. FnbA from S. aureus Newbould 305 (A) and RF122 (B) showing the approximate positions of the predicted fibronectin binding regions (FnbRs). High-affinity FnbRs are shaded. Signal peptide (S) in red, proline-rich repeats (PRR) in green; cell wall-spanning sequence (W) in white; membrane-spanning region (M) in white.

Mentions: Particular focus on microbial surface components recognizing adhesive matrix molecule (MSCRAMMs) genes revealed that their number was higher in N305 than in RF122. Although both strains carry the gene coding for fibronectin-binding protein A (fnbA), we found that the fnbA sequence in N305 had two additional fibronectin-binding domains when compared to that of RF122 (Figure 2).Figure 2


Fine-tuned characterization of Staphylococcus aureus Newbould 305, a strain associated with mild and chronic mastitis in bovines.

Peton V, Bouchard DS, Almeida S, Rault L, Falentin H, Jardin J, Jan G, Hernandez D, François P, Schrenzel J, Azevedo V, Miyoshi A, Berkova N, Even S, Le Loir Y - Vet. Res. (2014)

Comparison of the FnbA found in RF122 and N305 with that ofS. aureus8325.4. The FnbA in S. aureus 8325.4 (SWISS-Prot P14738) contains 11 Fn-binding sites. FnbA from S. aureus Newbould 305 (A) and RF122 (B) showing the approximate positions of the predicted fibronectin binding regions (FnbRs). High-affinity FnbRs are shaded. Signal peptide (S) in red, proline-rich repeats (PRR) in green; cell wall-spanning sequence (W) in white; membrane-spanning region (M) in white.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4230361&req=5

Fig2: Comparison of the FnbA found in RF122 and N305 with that ofS. aureus8325.4. The FnbA in S. aureus 8325.4 (SWISS-Prot P14738) contains 11 Fn-binding sites. FnbA from S. aureus Newbould 305 (A) and RF122 (B) showing the approximate positions of the predicted fibronectin binding regions (FnbRs). High-affinity FnbRs are shaded. Signal peptide (S) in red, proline-rich repeats (PRR) in green; cell wall-spanning sequence (W) in white; membrane-spanning region (M) in white.
Mentions: Particular focus on microbial surface components recognizing adhesive matrix molecule (MSCRAMMs) genes revealed that their number was higher in N305 than in RF122. Although both strains carry the gene coding for fibronectin-binding protein A (fnbA), we found that the fnbA sequence in N305 had two additional fibronectin-binding domains when compared to that of RF122 (Figure 2).Figure 2

Bottom Line: The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide.In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells.N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. vincent.peton@rennes.inra.fr.

ABSTRACT
S. aureus is a major aetiological agent of ruminant mastitis worldwide. The chronic nature of S. aureus mastitis makes it difficult to cure and prone to resurgence. In order to identify the bacterial factors involved in this chronicity, Newbould 305 (N305), a strain that can reproducibly induce mild and chronic mastitis in an experimental setting, was characterized in depth. We employed genomic and proteomic techniques combined with phenotype characterization, in order to comprehensively analyse N305. The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide. Five mobile genetic elements were identified in the N305 genome as carrying virulence factors which correlated with phenotypic features such as cytotoxicity, mammary epithelial cell invasion or host-adaptation. In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells. N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential. Our data are consistent with the invasiveness and host-adaptation features which contribute to the chronicity of S. aureus mastitis. Mobile genetic elements, exoproteins and surface exposed proteins constitute good targets for further research to explore the underlying mechanisms related to mastitis chronicity.

No MeSH data available.


Related in: MedlinePlus