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Fine-tuned characterization of Staphylococcus aureus Newbould 305, a strain associated with mild and chronic mastitis in bovines.

Peton V, Bouchard DS, Almeida S, Rault L, Falentin H, Jardin J, Jan G, Hernandez D, François P, Schrenzel J, Azevedo V, Miyoshi A, Berkova N, Even S, Le Loir Y - Vet. Res. (2014)

Bottom Line: The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide.In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells.N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. vincent.peton@rennes.inra.fr.

ABSTRACT
S. aureus is a major aetiological agent of ruminant mastitis worldwide. The chronic nature of S. aureus mastitis makes it difficult to cure and prone to resurgence. In order to identify the bacterial factors involved in this chronicity, Newbould 305 (N305), a strain that can reproducibly induce mild and chronic mastitis in an experimental setting, was characterized in depth. We employed genomic and proteomic techniques combined with phenotype characterization, in order to comprehensively analyse N305. The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide. Five mobile genetic elements were identified in the N305 genome as carrying virulence factors which correlated with phenotypic features such as cytotoxicity, mammary epithelial cell invasion or host-adaptation. In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells. N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential. Our data are consistent with the invasiveness and host-adaptation features which contribute to the chronicity of S. aureus mastitis. Mobile genetic elements, exoproteins and surface exposed proteins constitute good targets for further research to explore the underlying mechanisms related to mastitis chronicity.

No MeSH data available.


Related in: MedlinePlus

Putative SaPIs found in the Newbould 305 genome. Putative SaPIs of N305 are presented in the upper lines, and compared with the most closely related SaPI found in public databases. A. Comparison of the pathogenicity island SaPIN305_1 with νSaβ in the RF122 and MW2 strains. B. Comparison of the pathogenicity island SaPIN305_2 with SaPIbov found in the S. aureus D30 strain [39]. C. Comparison of the pathogenicity island SaPIN305_3 with SaPIbov5 and SaPIbov4. D. Comparison of the pathogenicity island SaPIN305_4 with SaPIn2 found in the S. aureus strain N315 [40]. E. Comparison of the pathogenicity island SaPIN305_5 with SaPI2 found in S. aureus RN3984 [41]. Arrows represent open reading frames and their orientations. Orange: common genes shared with the most closely related SaPI represented on the Figure; blue: additional or different genes.
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Fig1: Putative SaPIs found in the Newbould 305 genome. Putative SaPIs of N305 are presented in the upper lines, and compared with the most closely related SaPI found in public databases. A. Comparison of the pathogenicity island SaPIN305_1 with νSaβ in the RF122 and MW2 strains. B. Comparison of the pathogenicity island SaPIN305_2 with SaPIbov found in the S. aureus D30 strain [39]. C. Comparison of the pathogenicity island SaPIN305_3 with SaPIbov5 and SaPIbov4. D. Comparison of the pathogenicity island SaPIN305_4 with SaPIn2 found in the S. aureus strain N315 [40]. E. Comparison of the pathogenicity island SaPIN305_5 with SaPI2 found in S. aureus RN3984 [41]. Arrows represent open reading frames and their orientations. Orange: common genes shared with the most closely related SaPI represented on the Figure; blue: additional or different genes.

Mentions: Analysis of the N305 genome using the PIPS pipeline [28] predicted the presence of five putative S. aureus Pathogenicity Islands (SaPIs). SaPI-N305_1 (contig 1) contained 35 predicted CDSs markedly similar to the CDSs of νSaβ already described in RF122 and S. aureus MW2 (Figure 1A). SaPI-N305_2 (contig 1) contained 15 CDSs markedly similar to a SaPIbov found in strain D30 (isolated in the context of human nasal carriage) [39] (Figure 1B). SaPI-N305_3 and SaPI-N305_4 were two adjacent putative SaPIs (contig 2) containing 19 and 28 CDSs, respectively. SaPI-N305_3 shared marked similarity with SaPIbov4 and SaPIbov5, which both carry a bovine variant of von Willebrand factor binding protein (vwbSbo5) (Figure 1C). SaPI-N305_4 shared similarity with SaPIn2, which was first described in the human S. aureus strain, N315 [40] (Figure 1D). SaPI-N305_5 (contig 6) contained 35 CDSs, 17 of which were found to be very similar to SaPI2. PIPS analysis also revealed the presence of two putative bacteriophages, on contig 5, containing 34 CDSs and 19 CDSs with homology to Bacteriophage 80alpha and bacteriophage 187, respectively.Figure 1


Fine-tuned characterization of Staphylococcus aureus Newbould 305, a strain associated with mild and chronic mastitis in bovines.

Peton V, Bouchard DS, Almeida S, Rault L, Falentin H, Jardin J, Jan G, Hernandez D, François P, Schrenzel J, Azevedo V, Miyoshi A, Berkova N, Even S, Le Loir Y - Vet. Res. (2014)

Putative SaPIs found in the Newbould 305 genome. Putative SaPIs of N305 are presented in the upper lines, and compared with the most closely related SaPI found in public databases. A. Comparison of the pathogenicity island SaPIN305_1 with νSaβ in the RF122 and MW2 strains. B. Comparison of the pathogenicity island SaPIN305_2 with SaPIbov found in the S. aureus D30 strain [39]. C. Comparison of the pathogenicity island SaPIN305_3 with SaPIbov5 and SaPIbov4. D. Comparison of the pathogenicity island SaPIN305_4 with SaPIn2 found in the S. aureus strain N315 [40]. E. Comparison of the pathogenicity island SaPIN305_5 with SaPI2 found in S. aureus RN3984 [41]. Arrows represent open reading frames and their orientations. Orange: common genes shared with the most closely related SaPI represented on the Figure; blue: additional or different genes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4230361&req=5

Fig1: Putative SaPIs found in the Newbould 305 genome. Putative SaPIs of N305 are presented in the upper lines, and compared with the most closely related SaPI found in public databases. A. Comparison of the pathogenicity island SaPIN305_1 with νSaβ in the RF122 and MW2 strains. B. Comparison of the pathogenicity island SaPIN305_2 with SaPIbov found in the S. aureus D30 strain [39]. C. Comparison of the pathogenicity island SaPIN305_3 with SaPIbov5 and SaPIbov4. D. Comparison of the pathogenicity island SaPIN305_4 with SaPIn2 found in the S. aureus strain N315 [40]. E. Comparison of the pathogenicity island SaPIN305_5 with SaPI2 found in S. aureus RN3984 [41]. Arrows represent open reading frames and their orientations. Orange: common genes shared with the most closely related SaPI represented on the Figure; blue: additional or different genes.
Mentions: Analysis of the N305 genome using the PIPS pipeline [28] predicted the presence of five putative S. aureus Pathogenicity Islands (SaPIs). SaPI-N305_1 (contig 1) contained 35 predicted CDSs markedly similar to the CDSs of νSaβ already described in RF122 and S. aureus MW2 (Figure 1A). SaPI-N305_2 (contig 1) contained 15 CDSs markedly similar to a SaPIbov found in strain D30 (isolated in the context of human nasal carriage) [39] (Figure 1B). SaPI-N305_3 and SaPI-N305_4 were two adjacent putative SaPIs (contig 2) containing 19 and 28 CDSs, respectively. SaPI-N305_3 shared marked similarity with SaPIbov4 and SaPIbov5, which both carry a bovine variant of von Willebrand factor binding protein (vwbSbo5) (Figure 1C). SaPI-N305_4 shared similarity with SaPIn2, which was first described in the human S. aureus strain, N315 [40] (Figure 1D). SaPI-N305_5 (contig 6) contained 35 CDSs, 17 of which were found to be very similar to SaPI2. PIPS analysis also revealed the presence of two putative bacteriophages, on contig 5, containing 34 CDSs and 19 CDSs with homology to Bacteriophage 80alpha and bacteriophage 187, respectively.Figure 1

Bottom Line: The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide.In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells.N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. vincent.peton@rennes.inra.fr.

ABSTRACT
S. aureus is a major aetiological agent of ruminant mastitis worldwide. The chronic nature of S. aureus mastitis makes it difficult to cure and prone to resurgence. In order to identify the bacterial factors involved in this chronicity, Newbould 305 (N305), a strain that can reproducibly induce mild and chronic mastitis in an experimental setting, was characterized in depth. We employed genomic and proteomic techniques combined with phenotype characterization, in order to comprehensively analyse N305. The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide. Five mobile genetic elements were identified in the N305 genome as carrying virulence factors which correlated with phenotypic features such as cytotoxicity, mammary epithelial cell invasion or host-adaptation. In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells. N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential. Our data are consistent with the invasiveness and host-adaptation features which contribute to the chronicity of S. aureus mastitis. Mobile genetic elements, exoproteins and surface exposed proteins constitute good targets for further research to explore the underlying mechanisms related to mastitis chronicity.

No MeSH data available.


Related in: MedlinePlus