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Glycosylinositol phosphorylceramides from Rosa cell cultures are boron-bridged in the plasma membrane and form complexes with rhamnogalacturonan II.

Voxeur A, Fry SC - Plant J. (2014)

Bottom Line: Using high-voltage paper electrophoresis, we showed that addition of GIPCs decreased the electrophoretic mobility of radiolabelled RG-II, suggesting formation of a GIPC-B-RG-II complex.We conclude that B plays a structural role in the plasma membrane.Finally, our results suggest a role for GIPCs in the RG-II dimerization process.

View Article: PubMed Central - PubMed

Affiliation: The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Edinburgh, EH9 3JH, UK.

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Co-expression network between genes involved in RG-II and glycosylinositol phosphorylceramide (GIPC) biosynthesis in various species using an edge-weighted force-directed approach, based on data retrieved from ATTED-II and visualised in Cytoscape 2.8 (http://www.cytoscape.org). Circles: white circle, LOH (encoding a ceramide synthase); pale grey, encoding enzymes involved in synthesis of RG-II side-chains; dark grey, encoding enzymes involved in synthesis of the homogalacturonan backbone; black, C4H (encoding cinnamate-4-hydroxylase, involved in lignin synthesis; included as a control). Lines: green lines, Arabidopsis thaliana; blue, Populus trichocarpa; red, Oryza sativa; black, Glycine max; yellow, Zea mays. Solid line, strong co-expression [mutual rank (MR) < 1000]; dotted line, weak co-expression (5000 > MR > 1000); no line, no transcriptomic data available or no co-expression (MR > 5000).
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fig06: Co-expression network between genes involved in RG-II and glycosylinositol phosphorylceramide (GIPC) biosynthesis in various species using an edge-weighted force-directed approach, based on data retrieved from ATTED-II and visualised in Cytoscape 2.8 (http://www.cytoscape.org). Circles: white circle, LOH (encoding a ceramide synthase); pale grey, encoding enzymes involved in synthesis of RG-II side-chains; dark grey, encoding enzymes involved in synthesis of the homogalacturonan backbone; black, C4H (encoding cinnamate-4-hydroxylase, involved in lignin synthesis; included as a control). Lines: green lines, Arabidopsis thaliana; blue, Populus trichocarpa; red, Oryza sativa; black, Glycine max; yellow, Zea mays. Solid line, strong co-expression [mutual rank (MR) < 1000]; dotted line, weak co-expression (5000 > MR > 1000); no line, no transcriptomic data available or no co-expression (MR > 5000).

Mentions: Results retrieved (Figure6) from Atted-II database (Obayashi et al., 2014) revealed that genes involved in GIPC biosynthesis, such as LAG ONE HOMOLOGUE (LOH) encoding a ceramide synthase (Ternes et al., 2011), were co-expressed in several species with genes involved in the biosynthesis of RG-II such as ketodeoxyoctulosonic acid [involved in biosynthesis of 3-deoxy-d-manno-2-octulosonic acid (KDO)] and RGXT (RG-II xylosyltransferase; involved in side-chain A biosynthesis). Likewise, LOH genes are co-expressed with genes involved in the biosynthesis of RG-II’s homogalacturonan backbone (GAUT1, GAUT7 and GAUT8, encoding three galacturonosyltransferases). Moreover, both RG-II and GIPC biosynthesis occur in the Golgi apparatus (Mohnen, 2008); therefore, if the B-dependent dimerization of RG-II does require the intermediacy of a GIPC–B–RG-II complex, all necessary participants in the process would be present together in the endo-membrane/exocytosis system – which is the major location of RG-II dimerization in vivo (Chormova et al., 2013).


Glycosylinositol phosphorylceramides from Rosa cell cultures are boron-bridged in the plasma membrane and form complexes with rhamnogalacturonan II.

Voxeur A, Fry SC - Plant J. (2014)

Co-expression network between genes involved in RG-II and glycosylinositol phosphorylceramide (GIPC) biosynthesis in various species using an edge-weighted force-directed approach, based on data retrieved from ATTED-II and visualised in Cytoscape 2.8 (http://www.cytoscape.org). Circles: white circle, LOH (encoding a ceramide synthase); pale grey, encoding enzymes involved in synthesis of RG-II side-chains; dark grey, encoding enzymes involved in synthesis of the homogalacturonan backbone; black, C4H (encoding cinnamate-4-hydroxylase, involved in lignin synthesis; included as a control). Lines: green lines, Arabidopsis thaliana; blue, Populus trichocarpa; red, Oryza sativa; black, Glycine max; yellow, Zea mays. Solid line, strong co-expression [mutual rank (MR) < 1000]; dotted line, weak co-expression (5000 > MR > 1000); no line, no transcriptomic data available or no co-expression (MR > 5000).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230332&req=5

fig06: Co-expression network between genes involved in RG-II and glycosylinositol phosphorylceramide (GIPC) biosynthesis in various species using an edge-weighted force-directed approach, based on data retrieved from ATTED-II and visualised in Cytoscape 2.8 (http://www.cytoscape.org). Circles: white circle, LOH (encoding a ceramide synthase); pale grey, encoding enzymes involved in synthesis of RG-II side-chains; dark grey, encoding enzymes involved in synthesis of the homogalacturonan backbone; black, C4H (encoding cinnamate-4-hydroxylase, involved in lignin synthesis; included as a control). Lines: green lines, Arabidopsis thaliana; blue, Populus trichocarpa; red, Oryza sativa; black, Glycine max; yellow, Zea mays. Solid line, strong co-expression [mutual rank (MR) < 1000]; dotted line, weak co-expression (5000 > MR > 1000); no line, no transcriptomic data available or no co-expression (MR > 5000).
Mentions: Results retrieved (Figure6) from Atted-II database (Obayashi et al., 2014) revealed that genes involved in GIPC biosynthesis, such as LAG ONE HOMOLOGUE (LOH) encoding a ceramide synthase (Ternes et al., 2011), were co-expressed in several species with genes involved in the biosynthesis of RG-II such as ketodeoxyoctulosonic acid [involved in biosynthesis of 3-deoxy-d-manno-2-octulosonic acid (KDO)] and RGXT (RG-II xylosyltransferase; involved in side-chain A biosynthesis). Likewise, LOH genes are co-expressed with genes involved in the biosynthesis of RG-II’s homogalacturonan backbone (GAUT1, GAUT7 and GAUT8, encoding three galacturonosyltransferases). Moreover, both RG-II and GIPC biosynthesis occur in the Golgi apparatus (Mohnen, 2008); therefore, if the B-dependent dimerization of RG-II does require the intermediacy of a GIPC–B–RG-II complex, all necessary participants in the process would be present together in the endo-membrane/exocytosis system – which is the major location of RG-II dimerization in vivo (Chormova et al., 2013).

Bottom Line: Using high-voltage paper electrophoresis, we showed that addition of GIPCs decreased the electrophoretic mobility of radiolabelled RG-II, suggesting formation of a GIPC-B-RG-II complex.We conclude that B plays a structural role in the plasma membrane.Finally, our results suggest a role for GIPCs in the RG-II dimerization process.

View Article: PubMed Central - PubMed

Affiliation: The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Edinburgh, EH9 3JH, UK.

Show MeSH
Related in: MedlinePlus