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Glycosylinositol phosphorylceramides from Rosa cell cultures are boron-bridged in the plasma membrane and form complexes with rhamnogalacturonan II.

Voxeur A, Fry SC - Plant J. (2014)

Bottom Line: Using high-voltage paper electrophoresis, we showed that addition of GIPCs decreased the electrophoretic mobility of radiolabelled RG-II, suggesting formation of a GIPC-B-RG-II complex.We conclude that B plays a structural role in the plasma membrane.Finally, our results suggest a role for GIPCs in the RG-II dimerization process.

View Article: PubMed Central - PubMed

Affiliation: The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Edinburgh, EH9 3JH, UK.

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Glycosylinositol phosphorylceramide (GIPC)-induced dimerization of monomeric RG-II. After 4 h of pre-treatment of monomeric RG-II without or with 1.2 mm H3BO3 ± GIPC or with 1.2 mm H3BO3 and 0.5 mm Pb(NO3)2, samples were analysed by polyacrylamide gel electrophoresis and silver stained. Abbreviations as in Figure4. Parts (a) and (b) show the results of two independent experiments.
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fig05: Glycosylinositol phosphorylceramide (GIPC)-induced dimerization of monomeric RG-II. After 4 h of pre-treatment of monomeric RG-II without or with 1.2 mm H3BO3 ± GIPC or with 1.2 mm H3BO3 and 0.5 mm Pb(NO3)2, samples were analysed by polyacrylamide gel electrophoresis and silver stained. Abbreviations as in Figure4. Parts (a) and (b) show the results of two independent experiments.

Mentions: In order to test whether GIPC plays a role in RG-II dimerization, we incubated GIPC with purified RG-II plus H3BO3 under conditions devised for demonstrating Pb2+-induced dimerization. After 4 h, the reaction mixture was analysed by polyacrylamide gel electrophoresis (PAGE) (Chormova et al., 2013). Some experiments showed that GIPC was able to enhance dimer formation as effectively as Pb2+in vitro (Figure5a). Other repeat experiments demonstrated a smaller effect of GIPC (Figure5b), although the dimerization rate was always higher with GIPC + H3BO3 than with H3BO3 alone.


Glycosylinositol phosphorylceramides from Rosa cell cultures are boron-bridged in the plasma membrane and form complexes with rhamnogalacturonan II.

Voxeur A, Fry SC - Plant J. (2014)

Glycosylinositol phosphorylceramide (GIPC)-induced dimerization of monomeric RG-II. After 4 h of pre-treatment of monomeric RG-II without or with 1.2 mm H3BO3 ± GIPC or with 1.2 mm H3BO3 and 0.5 mm Pb(NO3)2, samples were analysed by polyacrylamide gel electrophoresis and silver stained. Abbreviations as in Figure4. Parts (a) and (b) show the results of two independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230332&req=5

fig05: Glycosylinositol phosphorylceramide (GIPC)-induced dimerization of monomeric RG-II. After 4 h of pre-treatment of monomeric RG-II without or with 1.2 mm H3BO3 ± GIPC or with 1.2 mm H3BO3 and 0.5 mm Pb(NO3)2, samples were analysed by polyacrylamide gel electrophoresis and silver stained. Abbreviations as in Figure4. Parts (a) and (b) show the results of two independent experiments.
Mentions: In order to test whether GIPC plays a role in RG-II dimerization, we incubated GIPC with purified RG-II plus H3BO3 under conditions devised for demonstrating Pb2+-induced dimerization. After 4 h, the reaction mixture was analysed by polyacrylamide gel electrophoresis (PAGE) (Chormova et al., 2013). Some experiments showed that GIPC was able to enhance dimer formation as effectively as Pb2+in vitro (Figure5a). Other repeat experiments demonstrated a smaller effect of GIPC (Figure5b), although the dimerization rate was always higher with GIPC + H3BO3 than with H3BO3 alone.

Bottom Line: Using high-voltage paper electrophoresis, we showed that addition of GIPCs decreased the electrophoretic mobility of radiolabelled RG-II, suggesting formation of a GIPC-B-RG-II complex.We conclude that B plays a structural role in the plasma membrane.Finally, our results suggest a role for GIPCs in the RG-II dimerization process.

View Article: PubMed Central - PubMed

Affiliation: The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Edinburgh, EH9 3JH, UK.

Show MeSH
Related in: MedlinePlus