Glycosylinositol phosphorylceramides from Rosa cell cultures are boron-bridged in the plasma membrane and form complexes with rhamnogalacturonan II.
Bottom Line: Using high-voltage paper electrophoresis, we showed that addition of GIPCs decreased the electrophoretic mobility of radiolabelled RG-II, suggesting formation of a GIPC-B-RG-II complex.We conclude that B plays a structural role in the plasma membrane.Finally, our results suggest a role for GIPCs in the RG-II dimerization process.
Affiliation: The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Edinburgh, EH9 3JH, UK.Show MeSH
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Mentions: In order to test for possible RG-II–GIPC interactions, we tried to obtain GIPC as pure as possible by adapting the method of Buré et al. (2011). The main difference from the original protocol was the addition of a phase-partition step with methanol/chloroform/formic acid/water (Figure3a). As judged by TLC of the two phases obtained, the less polar lipids were found in the organic phase, the contaminating sugars in the AP and the GIPC at the interface (Figure3b). This interface material was then submitted to an acidic butanol/water phase partition, which removed polymers. The purified GIPC-containing BP was collected.
Affiliation: The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Edinburgh, EH9 3JH, UK.