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Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe₂O₃ nanoparticles.

Yan SY, Chen MM, Fan JG, Wang YQ, Du YQ, Hu Y, Xu LM - Braz. J. Med. Biol. Res. (2014)

Bottom Line: Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2.RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment.It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

ABSTRACT
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe₂O₃ nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe₂O₃ nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe₂O₃ MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe₂O₃ MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which appeared to be dependent on the concentration of the Fe₂O₃nanoparticles. Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2. RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment. It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase. Fe₂O₃ MFH can induce high Hsp70 expression at an early stage, enhance the expression of Bax, and decrease the expression of mutant p53, which promotes the apoptosis of tumor cells.

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RT-PCR detection of Hsp70 expression for SMMC-7721 cells after treatmentfor 12 h (A) and 24 h (B). NC: control groupA (distilled water only); UHG: spiking group (8 g/LFe2O3, no radiation); MFH4: MHF group with 8 g/LFe2O3; MFH3: MHF group with 6 g/LFe2O3; PC: control group B (MCF-7 cell group only);MFH2: MHF group with 4 g/L Fe2O3; MFH1: MHF group with 2g/L Fe2O3.
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f14: RT-PCR detection of Hsp70 expression for SMMC-7721 cells after treatmentfor 12 h (A) and 24 h (B). NC: control groupA (distilled water only); UHG: spiking group (8 g/LFe2O3, no radiation); MFH4: MHF group with 8 g/LFe2O3; MFH3: MHF group with 6 g/LFe2O3; PC: control group B (MCF-7 cell group only);MFH2: MHF group with 4 g/L Fe2O3; MFH1: MHF group with 2g/L Fe2O3.

Mentions: After hyperthermia treatment for 8-12 h, electrophoresis results showed that Hsp70and β-actin strips were not observed in control group A, the cells of the spikinggroup did not express Hsp70 but did express β-actin, and the target fragment was 240bp in size. For control group B, target fragments appeared at 240 and 348 bp,corresponding to β-actin and Hsp70, respectively. The electrophoresis images of theMFH group showed both β-actin and Hsp70 strips at 240 and 348 bp. The Hsp70 strip ofthe MFH group with 6 g/L Fe2O3 nanoparticle ferrofluid was thebrightest (Figure 14A).


Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe₂O₃ nanoparticles.

Yan SY, Chen MM, Fan JG, Wang YQ, Du YQ, Hu Y, Xu LM - Braz. J. Med. Biol. Res. (2014)

RT-PCR detection of Hsp70 expression for SMMC-7721 cells after treatmentfor 12 h (A) and 24 h (B). NC: control groupA (distilled water only); UHG: spiking group (8 g/LFe2O3, no radiation); MFH4: MHF group with 8 g/LFe2O3; MFH3: MHF group with 6 g/LFe2O3; PC: control group B (MCF-7 cell group only);MFH2: MHF group with 4 g/L Fe2O3; MFH1: MHF group with 2g/L Fe2O3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230284&req=5

f14: RT-PCR detection of Hsp70 expression for SMMC-7721 cells after treatmentfor 12 h (A) and 24 h (B). NC: control groupA (distilled water only); UHG: spiking group (8 g/LFe2O3, no radiation); MFH4: MHF group with 8 g/LFe2O3; MFH3: MHF group with 6 g/LFe2O3; PC: control group B (MCF-7 cell group only);MFH2: MHF group with 4 g/L Fe2O3; MFH1: MHF group with 2g/L Fe2O3.
Mentions: After hyperthermia treatment for 8-12 h, electrophoresis results showed that Hsp70and β-actin strips were not observed in control group A, the cells of the spikinggroup did not express Hsp70 but did express β-actin, and the target fragment was 240bp in size. For control group B, target fragments appeared at 240 and 348 bp,corresponding to β-actin and Hsp70, respectively. The electrophoresis images of theMFH group showed both β-actin and Hsp70 strips at 240 and 348 bp. The Hsp70 strip ofthe MFH group with 6 g/L Fe2O3 nanoparticle ferrofluid was thebrightest (Figure 14A).

Bottom Line: Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2.RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment.It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

ABSTRACT
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe₂O₃ nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe₂O₃ nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe₂O₃ MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe₂O₃ MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which appeared to be dependent on the concentration of the Fe₂O₃nanoparticles. Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2. RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment. It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase. Fe₂O₃ MFH can induce high Hsp70 expression at an early stage, enhance the expression of Bax, and decrease the expression of mutant p53, which promotes the apoptosis of tumor cells.

Show MeSH
Related in: MedlinePlus