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Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe₂O₃ nanoparticles.

Yan SY, Chen MM, Fan JG, Wang YQ, Du YQ, Hu Y, Xu LM - Braz. J. Med. Biol. Res. (2014)

Bottom Line: Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2.RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment.It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

ABSTRACT
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe₂O₃ nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe₂O₃ nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe₂O₃ MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe₂O₃ MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which appeared to be dependent on the concentration of the Fe₂O₃nanoparticles. Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2. RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment. It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase. Fe₂O₃ MFH can induce high Hsp70 expression at an early stage, enhance the expression of Bax, and decrease the expression of mutant p53, which promotes the apoptosis of tumor cells.

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Results of RNA electrophoresis of SMMC-7721 cells in 3 groups. CG:control group A (RPMI 1640 culture medium only); SMUHG: spiking group (8 g/LFe2O3, no radiation); MFHG: magnetic fluidhyperthermia group (lane 3: 2 g/LFe2O3; lane 4: 8 g/LFe2O3).
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f13: Results of RNA electrophoresis of SMMC-7721 cells in 3 groups. CG:control group A (RPMI 1640 culture medium only); SMUHG: spiking group (8 g/LFe2O3, no radiation); MFHG: magnetic fluidhyperthermia group (lane 3: 2 g/LFe2O3; lane 4: 8 g/LFe2O3).

Mentions: The A260 and A280 values of the RNA samples were measuredand the ratios for all samples were found to be greater than 1.8, which met therequirement for RNA extraction. The presence of three complete strips (5S, 18S,28S) following electrophoresis confirmed the RNA integrity (Figure 13).


Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe₂O₃ nanoparticles.

Yan SY, Chen MM, Fan JG, Wang YQ, Du YQ, Hu Y, Xu LM - Braz. J. Med. Biol. Res. (2014)

Results of RNA electrophoresis of SMMC-7721 cells in 3 groups. CG:control group A (RPMI 1640 culture medium only); SMUHG: spiking group (8 g/LFe2O3, no radiation); MFHG: magnetic fluidhyperthermia group (lane 3: 2 g/LFe2O3; lane 4: 8 g/LFe2O3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230284&req=5

f13: Results of RNA electrophoresis of SMMC-7721 cells in 3 groups. CG:control group A (RPMI 1640 culture medium only); SMUHG: spiking group (8 g/LFe2O3, no radiation); MFHG: magnetic fluidhyperthermia group (lane 3: 2 g/LFe2O3; lane 4: 8 g/LFe2O3).
Mentions: The A260 and A280 values of the RNA samples were measuredand the ratios for all samples were found to be greater than 1.8, which met therequirement for RNA extraction. The presence of three complete strips (5S, 18S,28S) following electrophoresis confirmed the RNA integrity (Figure 13).

Bottom Line: Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2.RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment.It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

ABSTRACT
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe₂O₃ nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe₂O₃ nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe₂O₃ MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe₂O₃ MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which appeared to be dependent on the concentration of the Fe₂O₃nanoparticles. Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2. RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment. It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase. Fe₂O₃ MFH can induce high Hsp70 expression at an early stage, enhance the expression of Bax, and decrease the expression of mutant p53, which promotes the apoptosis of tumor cells.

Show MeSH
Related in: MedlinePlus