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Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe₂O₃ nanoparticles.

Yan SY, Chen MM, Fan JG, Wang YQ, Du YQ, Hu Y, Xu LM - Braz. J. Med. Biol. Res. (2014)

Bottom Line: Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2.RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment.It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

ABSTRACT
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe₂O₃ nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe₂O₃ nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe₂O₃ MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe₂O₃ MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which appeared to be dependent on the concentration of the Fe₂O₃nanoparticles. Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2. RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment. It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase. Fe₂O₃ MFH can induce high Hsp70 expression at an early stage, enhance the expression of Bax, and decrease the expression of mutant p53, which promotes the apoptosis of tumor cells.

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Immunocytochemical images (400×) of Bax expression in SMMC-7721 hepatomacells. A, Control group A (RPMI 1640 culture medium only);B, spiking group (8 g/L Fe2O3, noradiation); C, 2 g/L Fe2O3 MFH group;D, 4 g/L Fe2O3 MFH group;E, 6 g/L Fe2O3 MFH group;F, 8 g/L Fe2O3 MFH group. MFH:magnetic fluid hyperthermia.
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f11: Immunocytochemical images (400×) of Bax expression in SMMC-7721 hepatomacells. A, Control group A (RPMI 1640 culture medium only);B, spiking group (8 g/L Fe2O3, noradiation); C, 2 g/L Fe2O3 MFH group;D, 4 g/L Fe2O3 MFH group;E, 6 g/L Fe2O3 MFH group;F, 8 g/L Fe2O3 MFH group. MFH:magnetic fluid hyperthermia.

Mentions: In the control group A, Bax and Bcl-2 had different levels of expression.Specifically, particulate red-stained areas were visible in the cytoplasm whereas noobvious staining reaction was observed in the nucleus. The spiking group was largelyidentical to the control group A. In the MFH group, increasing the ferrofluidconcentration significantly enhanced Bax expression while Bcl-2 expression changedlittle, and the staining was relatively light. Deposition of brownish magneticnanoparticles could be noted both inside and outside the tumor cells (Figures 11 and 12). The ratio of Bax/Bcl-2 appeared to increase with rising ferrofluidconcentration.


Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe₂O₃ nanoparticles.

Yan SY, Chen MM, Fan JG, Wang YQ, Du YQ, Hu Y, Xu LM - Braz. J. Med. Biol. Res. (2014)

Immunocytochemical images (400×) of Bax expression in SMMC-7721 hepatomacells. A, Control group A (RPMI 1640 culture medium only);B, spiking group (8 g/L Fe2O3, noradiation); C, 2 g/L Fe2O3 MFH group;D, 4 g/L Fe2O3 MFH group;E, 6 g/L Fe2O3 MFH group;F, 8 g/L Fe2O3 MFH group. MFH:magnetic fluid hyperthermia.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230284&req=5

f11: Immunocytochemical images (400×) of Bax expression in SMMC-7721 hepatomacells. A, Control group A (RPMI 1640 culture medium only);B, spiking group (8 g/L Fe2O3, noradiation); C, 2 g/L Fe2O3 MFH group;D, 4 g/L Fe2O3 MFH group;E, 6 g/L Fe2O3 MFH group;F, 8 g/L Fe2O3 MFH group. MFH:magnetic fluid hyperthermia.
Mentions: In the control group A, Bax and Bcl-2 had different levels of expression.Specifically, particulate red-stained areas were visible in the cytoplasm whereas noobvious staining reaction was observed in the nucleus. The spiking group was largelyidentical to the control group A. In the MFH group, increasing the ferrofluidconcentration significantly enhanced Bax expression while Bcl-2 expression changedlittle, and the staining was relatively light. Deposition of brownish magneticnanoparticles could be noted both inside and outside the tumor cells (Figures 11 and 12). The ratio of Bax/Bcl-2 appeared to increase with rising ferrofluidconcentration.

Bottom Line: Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2.RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment.It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

ABSTRACT
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe₂O₃ nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe₂O₃ nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe₂O₃ MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe₂O₃ MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which appeared to be dependent on the concentration of the Fe₂O₃nanoparticles. Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2. RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment. It can be concluded that Fe₂O₃MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G₂/M phase. Fe₂O₃ MFH can induce high Hsp70 expression at an early stage, enhance the expression of Bax, and decrease the expression of mutant p53, which promotes the apoptosis of tumor cells.

Show MeSH
Related in: MedlinePlus