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Prevalence of human enteroviruses among apparently healthy nursery school children in Accra.

Attoh J, Obodai E, Adiku T, Odoom JK - Pan Afr Med J (2014)

Bottom Line: No growth was seen on L20B even after blind passage.More than half of the isolates could not be typed by the antisera pools.BD school was found to have the highest prevalence of NPEV.

View Article: PubMed Central - PubMed

Affiliation: Department Of Microbiology, University of Ghana Medical School, Legon, Ghana ; Ghana Health Service, Ministry of Health, Ghana.

ABSTRACT

Introduction: Human enteroviruses are common in children causing asymptomatic infections ranging from mild to severe illnesses. In Ghana, information on the prevalence of non-polio enterovirus causing acute flaccid paralysis is available but data on surveillance of these viruses in school children is scanty. Here, the prevalence of human enteroviruses among apparently healthy children in selected school in Accra was studied.

Methods: Stool samples from 273 apparently healthy children less than eight years of age in 9 selected nursery schools were collected between December 2010 and March 2011 and processed for human enteroviruses on L20B, RD and Hep-2 cell lines. Positive Isolates were characterized by microneutralisation assay with antisera pools from RIVM, the Netherlands according to standard methods recommended by WHO.

Results: Of the 273 samples processed, 66 (24.2%) non-polio enteroviruses were isolated. More growth was seen on Hep-2C (46%) only than RD (18%) only and on both cell lines (34%). No growth was seen on L20B even after blind passage. Excretion of non-polio enteroviruses was found in all the schools with majority in BD school. Serotyping of the isolates yielded predominantly Coxsackie B viruses followed by echoviruses 13 and 7. More than half of the isolates could not be typed by the antisera pools.

Conclusion: The study detected 13 different serotypes of non-polio enteroviruses in circulation but no poliovirus was found. BD school was found to have the highest prevalence of NPEV. Complete identification through molecular methods is essential to establish the full range of NPEVs in circulation in these schools.

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Number of isolates positive on different RD and Hep-2C cell lines by school
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Figure 0002: Number of isolates positive on different RD and Hep-2C cell lines by school

Mentions: Stool suspensions from 273 apparently healthy children from 9 kindergarten schools were inoculated on L20B, RD and Hep-2C cell lines. A total of 66 (24.2%) isolates were obtained with 28 (42%) from males. There was a higher isolation rates (56%) among younger children aged two and below than in older children between the ages of 3 years and above. Of these, 31 (11.4%) and 12 (4.4%) respectively showed growth on Hep-2C and RD cells only while 23 (8.4%) showed growth on both cell lines. There was no isolation on L20B cell line. Most (25.8%) of the isolates were obtained from BD school with the least (1.5%) from KM school as shown in Figure 1. Substantial amount of isolates were also obtained from MS, MU and TS schools. As shown in Table 1, majority 47% of the isolates were harvested on Hep-2C only compared with RD cell line while 34.8% were collected on both RD and Hep-2C. The results also indicated that isolates were made on Hep-2C from all the 9 schools while isolates on RD cells were harvested from 6 schools (Figure 2).


Prevalence of human enteroviruses among apparently healthy nursery school children in Accra.

Attoh J, Obodai E, Adiku T, Odoom JK - Pan Afr Med J (2014)

Number of isolates positive on different RD and Hep-2C cell lines by school
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230227&req=5

Figure 0002: Number of isolates positive on different RD and Hep-2C cell lines by school
Mentions: Stool suspensions from 273 apparently healthy children from 9 kindergarten schools were inoculated on L20B, RD and Hep-2C cell lines. A total of 66 (24.2%) isolates were obtained with 28 (42%) from males. There was a higher isolation rates (56%) among younger children aged two and below than in older children between the ages of 3 years and above. Of these, 31 (11.4%) and 12 (4.4%) respectively showed growth on Hep-2C and RD cells only while 23 (8.4%) showed growth on both cell lines. There was no isolation on L20B cell line. Most (25.8%) of the isolates were obtained from BD school with the least (1.5%) from KM school as shown in Figure 1. Substantial amount of isolates were also obtained from MS, MU and TS schools. As shown in Table 1, majority 47% of the isolates were harvested on Hep-2C only compared with RD cell line while 34.8% were collected on both RD and Hep-2C. The results also indicated that isolates were made on Hep-2C from all the 9 schools while isolates on RD cells were harvested from 6 schools (Figure 2).

Bottom Line: No growth was seen on L20B even after blind passage.More than half of the isolates could not be typed by the antisera pools.BD school was found to have the highest prevalence of NPEV.

View Article: PubMed Central - PubMed

Affiliation: Department Of Microbiology, University of Ghana Medical School, Legon, Ghana ; Ghana Health Service, Ministry of Health, Ghana.

ABSTRACT

Introduction: Human enteroviruses are common in children causing asymptomatic infections ranging from mild to severe illnesses. In Ghana, information on the prevalence of non-polio enterovirus causing acute flaccid paralysis is available but data on surveillance of these viruses in school children is scanty. Here, the prevalence of human enteroviruses among apparently healthy children in selected school in Accra was studied.

Methods: Stool samples from 273 apparently healthy children less than eight years of age in 9 selected nursery schools were collected between December 2010 and March 2011 and processed for human enteroviruses on L20B, RD and Hep-2 cell lines. Positive Isolates were characterized by microneutralisation assay with antisera pools from RIVM, the Netherlands according to standard methods recommended by WHO.

Results: Of the 273 samples processed, 66 (24.2%) non-polio enteroviruses were isolated. More growth was seen on Hep-2C (46%) only than RD (18%) only and on both cell lines (34%). No growth was seen on L20B even after blind passage. Excretion of non-polio enteroviruses was found in all the schools with majority in BD school. Serotyping of the isolates yielded predominantly Coxsackie B viruses followed by echoviruses 13 and 7. More than half of the isolates could not be typed by the antisera pools.

Conclusion: The study detected 13 different serotypes of non-polio enteroviruses in circulation but no poliovirus was found. BD school was found to have the highest prevalence of NPEV. Complete identification through molecular methods is essential to establish the full range of NPEVs in circulation in these schools.

Show MeSH
Related in: MedlinePlus