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Collyricloides massanae (Digenea, Collyriclidae): spermatozoon ultrastructure and phylogenetic importance.

Bakhoum AJ, Quilichini Y, Miquel J, Feliu C, Bâ CT, Marchand B - Parasite (2014)

Bottom Line: The spermatological characteristics of Collyricloides massanae (Digenea: Collyriclidae), a parasite of Apodemus sylvaticus caught in France, were studied by means of transmission electron microscopy.The ultrastructural criteria described in C. massanae such as the morphology of both anterior and posterior spermatozoon extremities, the association "external ornamentation+cortical microtubules", the type 2 of external ornamentation and the spine-like bodies would allow us to bring closer the Collyriclidae to Microphalloidea.However, further ultrastructural and molecular studies are needed particularly in the unexplored taxa in order to fully resolve the phylogenetic position of the Collyriclidae.

View Article: PubMed Central - PubMed

Affiliation: CNRS - University of Corsica, UMR SPE 6134, SERME "Service d'Étude et de Recherche en Microscopie Électronique", 20250 Corte, France - Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, BP 5055, Dakar, Senegal.

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Mature spermatozoon of Collyricloides massanae. (a) Middle part of Region IV characterised by the disorganisation of the first axoneme into doublets (D). M, mitochondrion; N, nucleus. (b) Cross-section showing one axoneme, mitochondrion (M), nucleus (N) and cortical microtubules (CM). (c) Cross-section after mitochondrion disappearance exhibiting only nucleus (N), axoneme and few cortical microtubules (CM). (d, e) Consecutive cross-sections in the distal part of Region IV showing progressive decrease of nucleus diameter and appearance of “cytoplasmic stalk” (CS) between the nucleus (N) and the axoneme (Ax). Note also the disappearance of cortical microtubules. (f) Posterior spermatozoon tip with doublet (D) of microtubules. (g) Cross-sections showing the granules of glycogen (G) evidenced by Thiéry’s cytochemical test [51]. Scale in μm: 0.3.
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Figure 3: Mature spermatozoon of Collyricloides massanae. (a) Middle part of Region IV characterised by the disorganisation of the first axoneme into doublets (D). M, mitochondrion; N, nucleus. (b) Cross-section showing one axoneme, mitochondrion (M), nucleus (N) and cortical microtubules (CM). (c) Cross-section after mitochondrion disappearance exhibiting only nucleus (N), axoneme and few cortical microtubules (CM). (d, e) Consecutive cross-sections in the distal part of Region IV showing progressive decrease of nucleus diameter and appearance of “cytoplasmic stalk” (CS) between the nucleus (N) and the axoneme (Ax). Note also the disappearance of cortical microtubules. (f) Posterior spermatozoon tip with doublet (D) of microtubules. (g) Cross-sections showing the granules of glycogen (G) evidenced by Thiéry’s cytochemical test [51]. Scale in μm: 0.3.

Mentions: Region III corresponds to the transitional area before the nuclear region. It shows only two axonemes, mitochondrion, cortical microtubules and granules of glycogen. Moreover, a decrease of maximum number of cortical microtubules from 10 (Figs. 2c and 4III) to 6 (Figs. 2d and 4III) can be seen.


Collyricloides massanae (Digenea, Collyriclidae): spermatozoon ultrastructure and phylogenetic importance.

Bakhoum AJ, Quilichini Y, Miquel J, Feliu C, Bâ CT, Marchand B - Parasite (2014)

Mature spermatozoon of Collyricloides massanae. (a) Middle part of Region IV characterised by the disorganisation of the first axoneme into doublets (D). M, mitochondrion; N, nucleus. (b) Cross-section showing one axoneme, mitochondrion (M), nucleus (N) and cortical microtubules (CM). (c) Cross-section after mitochondrion disappearance exhibiting only nucleus (N), axoneme and few cortical microtubules (CM). (d, e) Consecutive cross-sections in the distal part of Region IV showing progressive decrease of nucleus diameter and appearance of “cytoplasmic stalk” (CS) between the nucleus (N) and the axoneme (Ax). Note also the disappearance of cortical microtubules. (f) Posterior spermatozoon tip with doublet (D) of microtubules. (g) Cross-sections showing the granules of glycogen (G) evidenced by Thiéry’s cytochemical test [51]. Scale in μm: 0.3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230190&req=5

Figure 3: Mature spermatozoon of Collyricloides massanae. (a) Middle part of Region IV characterised by the disorganisation of the first axoneme into doublets (D). M, mitochondrion; N, nucleus. (b) Cross-section showing one axoneme, mitochondrion (M), nucleus (N) and cortical microtubules (CM). (c) Cross-section after mitochondrion disappearance exhibiting only nucleus (N), axoneme and few cortical microtubules (CM). (d, e) Consecutive cross-sections in the distal part of Region IV showing progressive decrease of nucleus diameter and appearance of “cytoplasmic stalk” (CS) between the nucleus (N) and the axoneme (Ax). Note also the disappearance of cortical microtubules. (f) Posterior spermatozoon tip with doublet (D) of microtubules. (g) Cross-sections showing the granules of glycogen (G) evidenced by Thiéry’s cytochemical test [51]. Scale in μm: 0.3.
Mentions: Region III corresponds to the transitional area before the nuclear region. It shows only two axonemes, mitochondrion, cortical microtubules and granules of glycogen. Moreover, a decrease of maximum number of cortical microtubules from 10 (Figs. 2c and 4III) to 6 (Figs. 2d and 4III) can be seen.

Bottom Line: The spermatological characteristics of Collyricloides massanae (Digenea: Collyriclidae), a parasite of Apodemus sylvaticus caught in France, were studied by means of transmission electron microscopy.The ultrastructural criteria described in C. massanae such as the morphology of both anterior and posterior spermatozoon extremities, the association "external ornamentation+cortical microtubules", the type 2 of external ornamentation and the spine-like bodies would allow us to bring closer the Collyriclidae to Microphalloidea.However, further ultrastructural and molecular studies are needed particularly in the unexplored taxa in order to fully resolve the phylogenetic position of the Collyriclidae.

View Article: PubMed Central - PubMed

Affiliation: CNRS - University of Corsica, UMR SPE 6134, SERME "Service d'Étude et de Recherche en Microscopie Électronique", 20250 Corte, France - Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, BP 5055, Dakar, Senegal.

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