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Collyricloides massanae (Digenea, Collyriclidae): spermatozoon ultrastructure and phylogenetic importance.

Bakhoum AJ, Quilichini Y, Miquel J, Feliu C, Bâ CT, Marchand B - Parasite (2014)

Bottom Line: The spermatological characteristics of Collyricloides massanae (Digenea: Collyriclidae), a parasite of Apodemus sylvaticus caught in France, were studied by means of transmission electron microscopy.The ultrastructural criteria described in C. massanae such as the morphology of both anterior and posterior spermatozoon extremities, the association "external ornamentation+cortical microtubules", the type 2 of external ornamentation and the spine-like bodies would allow us to bring closer the Collyriclidae to Microphalloidea.However, further ultrastructural and molecular studies are needed particularly in the unexplored taxa in order to fully resolve the phylogenetic position of the Collyriclidae.

View Article: PubMed Central - PubMed

Affiliation: CNRS - University of Corsica, UMR SPE 6134, SERME "Service d'Étude et de Recherche en Microscopie Électronique", 20250 Corte, France - Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, BP 5055, Dakar, Senegal.

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Mature spermatozoon of Collyricloides massanae. (a, b) Cross-sections in Region II or the ornamented zone of the mature spermatozoon exhibiting the mitochondrion (M), external ornamentation of the plasma membrane (EO) and spine-like body (SB). Note the association “external ornamentation + cortical microtubules”. (c, d) Region III or transitional area before nuclear appearance, exhibiting in cross-sections, only both axonemes, mitochondrion (M) and few cortical microtubules (CM). (e, f) Cross-sections in the proximal part of Region IV, showing the nucleus (N) accompanied by mitochondrion and cortical microtubules. Note the increase in size of the nucleus and its eccentric position. Scale in μm: 0.3.
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Figure 2: Mature spermatozoon of Collyricloides massanae. (a, b) Cross-sections in Region II or the ornamented zone of the mature spermatozoon exhibiting the mitochondrion (M), external ornamentation of the plasma membrane (EO) and spine-like body (SB). Note the association “external ornamentation + cortical microtubules”. (c, d) Region III or transitional area before nuclear appearance, exhibiting in cross-sections, only both axonemes, mitochondrion (M) and few cortical microtubules (CM). (e, f) Cross-sections in the proximal part of Region IV, showing the nucleus (N) accompanied by mitochondrion and cortical microtubules. Note the increase in size of the nucleus and its eccentric position. Scale in μm: 0.3.

Mentions: Mature spermatozoon of Collyricloides massanae. (a) Sharp morphology of the anterior spermatozoon extremity (ASE). (b, c) Cross-sections in Region I showing two centrioles (C1 and C2) corresponding to both axonemes and continuous layer of submembranous cortical microtubules (CM). Ax1, first axoneme. (d) Cross-section in which both axonemes are already formed and surrounded by a continuous layer of parallel cortical microtubules. (e, f) Consecutive cross-sections in the middle part of Region I exhibiting two and four attachment zones (arrows), interrupting the continuous layer of cortical microtubules. (g–i) Posterior part of Region I showing in cross-sections both axonemes and cortical microtubules organised into two fields separated by the four attachment zones. The appearance of the mitochondrion is also noticeable (M). Scale in μm: (a), 0.5; (b–i), 0.3.


Collyricloides massanae (Digenea, Collyriclidae): spermatozoon ultrastructure and phylogenetic importance.

Bakhoum AJ, Quilichini Y, Miquel J, Feliu C, Bâ CT, Marchand B - Parasite (2014)

Mature spermatozoon of Collyricloides massanae. (a, b) Cross-sections in Region II or the ornamented zone of the mature spermatozoon exhibiting the mitochondrion (M), external ornamentation of the plasma membrane (EO) and spine-like body (SB). Note the association “external ornamentation + cortical microtubules”. (c, d) Region III or transitional area before nuclear appearance, exhibiting in cross-sections, only both axonemes, mitochondrion (M) and few cortical microtubules (CM). (e, f) Cross-sections in the proximal part of Region IV, showing the nucleus (N) accompanied by mitochondrion and cortical microtubules. Note the increase in size of the nucleus and its eccentric position. Scale in μm: 0.3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230190&req=5

Figure 2: Mature spermatozoon of Collyricloides massanae. (a, b) Cross-sections in Region II or the ornamented zone of the mature spermatozoon exhibiting the mitochondrion (M), external ornamentation of the plasma membrane (EO) and spine-like body (SB). Note the association “external ornamentation + cortical microtubules”. (c, d) Region III or transitional area before nuclear appearance, exhibiting in cross-sections, only both axonemes, mitochondrion (M) and few cortical microtubules (CM). (e, f) Cross-sections in the proximal part of Region IV, showing the nucleus (N) accompanied by mitochondrion and cortical microtubules. Note the increase in size of the nucleus and its eccentric position. Scale in μm: 0.3.
Mentions: Mature spermatozoon of Collyricloides massanae. (a) Sharp morphology of the anterior spermatozoon extremity (ASE). (b, c) Cross-sections in Region I showing two centrioles (C1 and C2) corresponding to both axonemes and continuous layer of submembranous cortical microtubules (CM). Ax1, first axoneme. (d) Cross-section in which both axonemes are already formed and surrounded by a continuous layer of parallel cortical microtubules. (e, f) Consecutive cross-sections in the middle part of Region I exhibiting two and four attachment zones (arrows), interrupting the continuous layer of cortical microtubules. (g–i) Posterior part of Region I showing in cross-sections both axonemes and cortical microtubules organised into two fields separated by the four attachment zones. The appearance of the mitochondrion is also noticeable (M). Scale in μm: (a), 0.5; (b–i), 0.3.

Bottom Line: The spermatological characteristics of Collyricloides massanae (Digenea: Collyriclidae), a parasite of Apodemus sylvaticus caught in France, were studied by means of transmission electron microscopy.The ultrastructural criteria described in C. massanae such as the morphology of both anterior and posterior spermatozoon extremities, the association "external ornamentation+cortical microtubules", the type 2 of external ornamentation and the spine-like bodies would allow us to bring closer the Collyriclidae to Microphalloidea.However, further ultrastructural and molecular studies are needed particularly in the unexplored taxa in order to fully resolve the phylogenetic position of the Collyriclidae.

View Article: PubMed Central - PubMed

Affiliation: CNRS - University of Corsica, UMR SPE 6134, SERME "Service d'Étude et de Recherche en Microscopie Électronique", 20250 Corte, France - Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, BP 5055, Dakar, Senegal.

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