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Chromatin associations in Arabidopsis interphase nuclei.

Schubert V, Rudnik R, Schubert I - Front Genet (2014)

Bottom Line: We found that chromatin fiber movement and variable associations, although in general relatively seldom, may occur between euchromatin segments along chromosomes, sometimes even over large distances.The combination of euchromatin segments bearing high or low co-expressing genes did not reveal different association frequencies probably due to adjacent genes of deviating expression patterns.Based on previous data and on FISH analyses presented here, we conclude that the global interphase chromatin organization in A. thaliana is relatively stable, due to the location of its 10 centromeres at the nuclear periphery and of the telomeres mainly at the centrally localized nucleolus.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben Stadt Seeland, Germany.

ABSTRACT
The arrangement of chromatin within interphase nuclei seems to be caused by topological constraints and related to gene expression depending on tissue and developmental stage. In yeast and animals it was found that homologous and heterologous chromatin association are required to realize faithful expression and DNA repair. To test whether such associations are present in plants we analyzed Arabidopsis thaliana interphase nuclei by FISH using probes from different chromosomes. We found that chromatin fiber movement and variable associations, although in general relatively seldom, may occur between euchromatin segments along chromosomes, sometimes even over large distances. The combination of euchromatin segments bearing high or low co-expressing genes did not reveal different association frequencies probably due to adjacent genes of deviating expression patterns. Based on previous data and on FISH analyses presented here, we conclude that the global interphase chromatin organization in A. thaliana is relatively stable, due to the location of its 10 centromeres at the nuclear periphery and of the telomeres mainly at the centrally localized nucleolus. Nevertheless, chromatin movement enables a flexible spatial genome arrangement in plant nuclei.

No MeSH data available.


Chromatin dynamics in living Tradescantia paludosa nuclei. (A) The position of nuclei in stamen hair cells (A1) is kept by attached plasma fiber strands (pfs) (A2). (B) Compared to the starting point of time (B1) the chromatin fibers mainly keep their position (arrows) after 70 min (B2) and 140 min (B3). But in addition, other vesicular structures (asterisks) appear.
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Figure 6: Chromatin dynamics in living Tradescantia paludosa nuclei. (A) The position of nuclei in stamen hair cells (A1) is kept by attached plasma fiber strands (pfs) (A2). (B) Compared to the starting point of time (B1) the chromatin fibers mainly keep their position (arrows) after 70 min (B2) and 140 min (B3). But in addition, other vesicular structures (asterisks) appear.

Mentions: We used DIC microscopy to analyse the dynamics of chromatin in interphase nuclei of Tradescantia stamen hairs. During 120 min we observed that the nuclei attached to plasma fibers move and change their shape only slightly. Also the chromatin fibers inside keep mainly their position. But in addition, other vesicular structures (possibly nuclear bodies containing components involved in splicing, transcription or gene silencing; Del Prete et al., 2014) appear (Figure 6; Suppl. movie 1). Thus, we confirm also in living tissue that plant interphase nuclei maintain their global chromatin organization for longer times in spite of showing some flexibility.


Chromatin associations in Arabidopsis interphase nuclei.

Schubert V, Rudnik R, Schubert I - Front Genet (2014)

Chromatin dynamics in living Tradescantia paludosa nuclei. (A) The position of nuclei in stamen hair cells (A1) is kept by attached plasma fiber strands (pfs) (A2). (B) Compared to the starting point of time (B1) the chromatin fibers mainly keep their position (arrows) after 70 min (B2) and 140 min (B3). But in addition, other vesicular structures (asterisks) appear.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230181&req=5

Figure 6: Chromatin dynamics in living Tradescantia paludosa nuclei. (A) The position of nuclei in stamen hair cells (A1) is kept by attached plasma fiber strands (pfs) (A2). (B) Compared to the starting point of time (B1) the chromatin fibers mainly keep their position (arrows) after 70 min (B2) and 140 min (B3). But in addition, other vesicular structures (asterisks) appear.
Mentions: We used DIC microscopy to analyse the dynamics of chromatin in interphase nuclei of Tradescantia stamen hairs. During 120 min we observed that the nuclei attached to plasma fibers move and change their shape only slightly. Also the chromatin fibers inside keep mainly their position. But in addition, other vesicular structures (possibly nuclear bodies containing components involved in splicing, transcription or gene silencing; Del Prete et al., 2014) appear (Figure 6; Suppl. movie 1). Thus, we confirm also in living tissue that plant interphase nuclei maintain their global chromatin organization for longer times in spite of showing some flexibility.

Bottom Line: We found that chromatin fiber movement and variable associations, although in general relatively seldom, may occur between euchromatin segments along chromosomes, sometimes even over large distances.The combination of euchromatin segments bearing high or low co-expressing genes did not reveal different association frequencies probably due to adjacent genes of deviating expression patterns.Based on previous data and on FISH analyses presented here, we conclude that the global interphase chromatin organization in A. thaliana is relatively stable, due to the location of its 10 centromeres at the nuclear periphery and of the telomeres mainly at the centrally localized nucleolus.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben Stadt Seeland, Germany.

ABSTRACT
The arrangement of chromatin within interphase nuclei seems to be caused by topological constraints and related to gene expression depending on tissue and developmental stage. In yeast and animals it was found that homologous and heterologous chromatin association are required to realize faithful expression and DNA repair. To test whether such associations are present in plants we analyzed Arabidopsis thaliana interphase nuclei by FISH using probes from different chromosomes. We found that chromatin fiber movement and variable associations, although in general relatively seldom, may occur between euchromatin segments along chromosomes, sometimes even over large distances. The combination of euchromatin segments bearing high or low co-expressing genes did not reveal different association frequencies probably due to adjacent genes of deviating expression patterns. Based on previous data and on FISH analyses presented here, we conclude that the global interphase chromatin organization in A. thaliana is relatively stable, due to the location of its 10 centromeres at the nuclear periphery and of the telomeres mainly at the centrally localized nucleolus. Nevertheless, chromatin movement enables a flexible spatial genome arrangement in plant nuclei.

No MeSH data available.