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Immunosuppressive Effects of A-Type Procyanidin Oligomers from Cinnamomum tamala.

Chen L, Yang Y, Yuan P, Yang Y, Chen K, Jia Q, Li Y - Evid Based Complement Alternat Med (2014)

Bottom Line: Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear.Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1.CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine (SHUTCM), Shanghai 201203, China.

ABSTRACT
Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear. The aim of this study is to find out the possible component(s) that can be used as therapeutic agents for immune-related diseases from cinnamon bark. In this study, the immunosuppressive effects of fraction (named CT-F) and five procyanidin oligomers compounds, cinnamtannin B1, cinnamtannin D1 (CTD-1), parameritannin A1, procyanidin B2, and procyanidin C1, from Cinnamomum tamala or Cinnamomum cassia bark were examined on splenocytes proliferation model induced by ConA or LPS. Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1. It was found that CT-F and CTD-1 significantly inhibited the splenocyte proliferation induced by ConA or LPS. CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses. These findings suggest that the immunosuppressive activities of cinnamon bark are in part due to procyanidin oligomers. CTD-1 may be a potential therapeutic agent for immune-related diseases.

No MeSH data available.


Related in: MedlinePlus

Effects of CTD-1 on DTH responses in BALB/c mice. BALB/c mice were initially sensitized with DNFB on days 0 and 1 and then challenged with DNFB on day 9. Vehicle and CTD-1 were administered after the first challenge. Ear swelling was calculated as the difference between the weights (a) or thickness (b) of left (DNFB treated) and right (untreated) ear punches 24 h after challenge. Treatment by CSA (1 mg/Kg) was set as positive group. Data are expressed as mean ± S.D. *P < 0.05, **P < 0.01, n = 5 mice/group.
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fig7: Effects of CTD-1 on DTH responses in BALB/c mice. BALB/c mice were initially sensitized with DNFB on days 0 and 1 and then challenged with DNFB on day 9. Vehicle and CTD-1 were administered after the first challenge. Ear swelling was calculated as the difference between the weights (a) or thickness (b) of left (DNFB treated) and right (untreated) ear punches 24 h after challenge. Treatment by CSA (1 mg/Kg) was set as positive group. Data are expressed as mean ± S.D. *P < 0.05, **P < 0.01, n = 5 mice/group.

Mentions: The effects of CTD-1 on the production of Th1-type cytokines IL-2 and IFN-γ and effect on DTH responses in BALB/c mice were examined. As shown in Figure 6, IL-2 and IFN-γ productions were significantly decreased when the splenocyte cultures were exposed to CTD-1. In in vivo experiment, in DNFB-induced DTH, CTD-1 significantly suppressed ear weight (thickness) by 8.9% (7.6%), 29.4% (10.7%), and 36.2% (29.2%) at the dosage of 3.3, 10, and 20 μM, respectively. These results suggested that CTD-1 might protect hosts from inflammatory reactions via strong inhibition of T cell functions (Figure 7).


Immunosuppressive Effects of A-Type Procyanidin Oligomers from Cinnamomum tamala.

Chen L, Yang Y, Yuan P, Yang Y, Chen K, Jia Q, Li Y - Evid Based Complement Alternat Med (2014)

Effects of CTD-1 on DTH responses in BALB/c mice. BALB/c mice were initially sensitized with DNFB on days 0 and 1 and then challenged with DNFB on day 9. Vehicle and CTD-1 were administered after the first challenge. Ear swelling was calculated as the difference between the weights (a) or thickness (b) of left (DNFB treated) and right (untreated) ear punches 24 h after challenge. Treatment by CSA (1 mg/Kg) was set as positive group. Data are expressed as mean ± S.D. *P < 0.05, **P < 0.01, n = 5 mice/group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230008&req=5

fig7: Effects of CTD-1 on DTH responses in BALB/c mice. BALB/c mice were initially sensitized with DNFB on days 0 and 1 and then challenged with DNFB on day 9. Vehicle and CTD-1 were administered after the first challenge. Ear swelling was calculated as the difference between the weights (a) or thickness (b) of left (DNFB treated) and right (untreated) ear punches 24 h after challenge. Treatment by CSA (1 mg/Kg) was set as positive group. Data are expressed as mean ± S.D. *P < 0.05, **P < 0.01, n = 5 mice/group.
Mentions: The effects of CTD-1 on the production of Th1-type cytokines IL-2 and IFN-γ and effect on DTH responses in BALB/c mice were examined. As shown in Figure 6, IL-2 and IFN-γ productions were significantly decreased when the splenocyte cultures were exposed to CTD-1. In in vivo experiment, in DNFB-induced DTH, CTD-1 significantly suppressed ear weight (thickness) by 8.9% (7.6%), 29.4% (10.7%), and 36.2% (29.2%) at the dosage of 3.3, 10, and 20 μM, respectively. These results suggested that CTD-1 might protect hosts from inflammatory reactions via strong inhibition of T cell functions (Figure 7).

Bottom Line: Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear.Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1.CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine (SHUTCM), Shanghai 201203, China.

ABSTRACT
Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear. The aim of this study is to find out the possible component(s) that can be used as therapeutic agents for immune-related diseases from cinnamon bark. In this study, the immunosuppressive effects of fraction (named CT-F) and five procyanidin oligomers compounds, cinnamtannin B1, cinnamtannin D1 (CTD-1), parameritannin A1, procyanidin B2, and procyanidin C1, from Cinnamomum tamala or Cinnamomum cassia bark were examined on splenocytes proliferation model induced by ConA or LPS. Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1. It was found that CT-F and CTD-1 significantly inhibited the splenocyte proliferation induced by ConA or LPS. CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses. These findings suggest that the immunosuppressive activities of cinnamon bark are in part due to procyanidin oligomers. CTD-1 may be a potential therapeutic agent for immune-related diseases.

No MeSH data available.


Related in: MedlinePlus