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Immunosuppressive Effects of A-Type Procyanidin Oligomers from Cinnamomum tamala.

Chen L, Yang Y, Yuan P, Yang Y, Chen K, Jia Q, Li Y - Evid Based Complement Alternat Med (2014)

Bottom Line: Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear.Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1.CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine (SHUTCM), Shanghai 201203, China.

ABSTRACT
Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear. The aim of this study is to find out the possible component(s) that can be used as therapeutic agents for immune-related diseases from cinnamon bark. In this study, the immunosuppressive effects of fraction (named CT-F) and five procyanidin oligomers compounds, cinnamtannin B1, cinnamtannin D1 (CTD-1), parameritannin A1, procyanidin B2, and procyanidin C1, from Cinnamomum tamala or Cinnamomum cassia bark were examined on splenocytes proliferation model induced by ConA or LPS. Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1. It was found that CT-F and CTD-1 significantly inhibited the splenocyte proliferation induced by ConA or LPS. CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses. These findings suggest that the immunosuppressive activities of cinnamon bark are in part due to procyanidin oligomers. CTD-1 may be a potential therapeutic agent for immune-related diseases.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity on splenocytes and inhibition on ConA or LPS induced splenocyte proliferation of CTD-1. (a) Cytotoxicity of CTD-1 on BALB/c mice splenocytes. The cells were incubated with different concentration of CTD-1 for 48 h. And the cell viability was measured by MTT assay. ((b) and (c)) Inhibition of CTD-1 on splenocyte proliferation induced by ConA (b) or LPS (c). BALB/c mice splenocytes (4 × 105 cells/well) were stimulated by ConA (2 μg/mL) or LPS (10 μg/mL) for 48 h in the presence of CTD-1. Cells were then pulsed with 0.25 μCi [3H]thymidine 8 h before the end of the experiment and were assessed for [3H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. *P < 0.05, **P < 0.01, and ***P < 0.001, treatment group versus control.
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fig5: Cytotoxicity on splenocytes and inhibition on ConA or LPS induced splenocyte proliferation of CTD-1. (a) Cytotoxicity of CTD-1 on BALB/c mice splenocytes. The cells were incubated with different concentration of CTD-1 for 48 h. And the cell viability was measured by MTT assay. ((b) and (c)) Inhibition of CTD-1 on splenocyte proliferation induced by ConA (b) or LPS (c). BALB/c mice splenocytes (4 × 105 cells/well) were stimulated by ConA (2 μg/mL) or LPS (10 μg/mL) for 48 h in the presence of CTD-1. Cells were then pulsed with 0.25 μCi [3H]thymidine 8 h before the end of the experiment and were assessed for [3H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. *P < 0.05, **P < 0.01, and ***P < 0.001, treatment group versus control.

Mentions: In order to further evaluate the immunosuppressive effect of CTD-1, two in vitro models, ConA and LPS induced splenocyte proliferation, were used in this experiment. The proliferation was suppressed in the CTD-1 treated splenocyte under ConA or LPS induction in a concentration-dependent manner (Figures 5(b) and 5(c)). The values of 4.38 ± 0.12 μM and 6.62 ± 1.04 μM correspond to two models induced by ConA and LPS. So the different of two values is reasonable.


Immunosuppressive Effects of A-Type Procyanidin Oligomers from Cinnamomum tamala.

Chen L, Yang Y, Yuan P, Yang Y, Chen K, Jia Q, Li Y - Evid Based Complement Alternat Med (2014)

Cytotoxicity on splenocytes and inhibition on ConA or LPS induced splenocyte proliferation of CTD-1. (a) Cytotoxicity of CTD-1 on BALB/c mice splenocytes. The cells were incubated with different concentration of CTD-1 for 48 h. And the cell viability was measured by MTT assay. ((b) and (c)) Inhibition of CTD-1 on splenocyte proliferation induced by ConA (b) or LPS (c). BALB/c mice splenocytes (4 × 105 cells/well) were stimulated by ConA (2 μg/mL) or LPS (10 μg/mL) for 48 h in the presence of CTD-1. Cells were then pulsed with 0.25 μCi [3H]thymidine 8 h before the end of the experiment and were assessed for [3H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. *P < 0.05, **P < 0.01, and ***P < 0.001, treatment group versus control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230008&req=5

fig5: Cytotoxicity on splenocytes and inhibition on ConA or LPS induced splenocyte proliferation of CTD-1. (a) Cytotoxicity of CTD-1 on BALB/c mice splenocytes. The cells were incubated with different concentration of CTD-1 for 48 h. And the cell viability was measured by MTT assay. ((b) and (c)) Inhibition of CTD-1 on splenocyte proliferation induced by ConA (b) or LPS (c). BALB/c mice splenocytes (4 × 105 cells/well) were stimulated by ConA (2 μg/mL) or LPS (10 μg/mL) for 48 h in the presence of CTD-1. Cells were then pulsed with 0.25 μCi [3H]thymidine 8 h before the end of the experiment and were assessed for [3H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. *P < 0.05, **P < 0.01, and ***P < 0.001, treatment group versus control.
Mentions: In order to further evaluate the immunosuppressive effect of CTD-1, two in vitro models, ConA and LPS induced splenocyte proliferation, were used in this experiment. The proliferation was suppressed in the CTD-1 treated splenocyte under ConA or LPS induction in a concentration-dependent manner (Figures 5(b) and 5(c)). The values of 4.38 ± 0.12 μM and 6.62 ± 1.04 μM correspond to two models induced by ConA and LPS. So the different of two values is reasonable.

Bottom Line: Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear.Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1.CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine (SHUTCM), Shanghai 201203, China.

ABSTRACT
Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear. The aim of this study is to find out the possible component(s) that can be used as therapeutic agents for immune-related diseases from cinnamon bark. In this study, the immunosuppressive effects of fraction (named CT-F) and five procyanidin oligomers compounds, cinnamtannin B1, cinnamtannin D1 (CTD-1), parameritannin A1, procyanidin B2, and procyanidin C1, from Cinnamomum tamala or Cinnamomum cassia bark were examined on splenocytes proliferation model induced by ConA or LPS. Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1. It was found that CT-F and CTD-1 significantly inhibited the splenocyte proliferation induced by ConA or LPS. CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses. These findings suggest that the immunosuppressive activities of cinnamon bark are in part due to procyanidin oligomers. CTD-1 may be a potential therapeutic agent for immune-related diseases.

No MeSH data available.


Related in: MedlinePlus