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Immunosuppressive Effects of A-Type Procyanidin Oligomers from Cinnamomum tamala.

Chen L, Yang Y, Yuan P, Yang Y, Chen K, Jia Q, Li Y - Evid Based Complement Alternat Med (2014)

Bottom Line: Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear.Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1.CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine (SHUTCM), Shanghai 201203, China.

ABSTRACT
Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear. The aim of this study is to find out the possible component(s) that can be used as therapeutic agents for immune-related diseases from cinnamon bark. In this study, the immunosuppressive effects of fraction (named CT-F) and five procyanidin oligomers compounds, cinnamtannin B1, cinnamtannin D1 (CTD-1), parameritannin A1, procyanidin B2, and procyanidin C1, from Cinnamomum tamala or Cinnamomum cassia bark were examined on splenocytes proliferation model induced by ConA or LPS. Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1. It was found that CT-F and CTD-1 significantly inhibited the splenocyte proliferation induced by ConA or LPS. CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses. These findings suggest that the immunosuppressive activities of cinnamon bark are in part due to procyanidin oligomers. CTD-1 may be a potential therapeutic agent for immune-related diseases.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity on splenocytes and inhibition on ConA-induced splenocyte proliferation of five procyanidin compounds 1–5. (a) Cytotoxicity of procyanidin compounds on BALB/c mice splenocytes. The cells were incubated with different concentration of procyanidin compounds for 48 h. And the cell viability was measured by MTT assay. (b) Inhibition of procyanidin compounds on ConA-induced splenocyte proliferation. BALB/c mice splenocytes (4 × 105 cells/well) were stimulated by ConA (2 μg/mL) for 48 h in the presence of different procyanidin compounds. Cells were then pulsed with 0.25 μCi [3H]thymidine 8 h before the end of the experiment and were assessed for [3H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. *P < 0.05, **P < 0.01, treatment group versus control.
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fig4: Cytotoxicity on splenocytes and inhibition on ConA-induced splenocyte proliferation of five procyanidin compounds 1–5. (a) Cytotoxicity of procyanidin compounds on BALB/c mice splenocytes. The cells were incubated with different concentration of procyanidin compounds for 48 h. And the cell viability was measured by MTT assay. (b) Inhibition of procyanidin compounds on ConA-induced splenocyte proliferation. BALB/c mice splenocytes (4 × 105 cells/well) were stimulated by ConA (2 μg/mL) for 48 h in the presence of different procyanidin compounds. Cells were then pulsed with 0.25 μCi [3H]thymidine 8 h before the end of the experiment and were assessed for [3H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. *P < 0.05, **P < 0.01, treatment group versus control.

Mentions: The immunosuppressive effects of the five procyanidins were determined using the same method (Figure 4). It was found that all five test compounds have no cytotoxic effect at concentrations from 0.16 μM to 20 μM (Figure 4(a)). Figure 4(b) showed that, even at a low concentration of 0.16 μM, CTD-1 can inhibit ConA-induced splenocyte proliferation by 40%, suggesting that CTD-1 produced the best effects of all compounds. Other A-type procyanidin oligomers CTB-1 and PA-1 significantly suppressed ConA-induced splenocyte proliferation at 20 μM. However, B-type procyanidin dimer PB-2 had no inhibitory effect even at 20 μM. Meanwhile, B-type procyanidin trimer PC-1 had weakly effect on ConA-induced splenocyte proliferation, only reducing it by 37% at a high concentration of 20 μM.


Immunosuppressive Effects of A-Type Procyanidin Oligomers from Cinnamomum tamala.

Chen L, Yang Y, Yuan P, Yang Y, Chen K, Jia Q, Li Y - Evid Based Complement Alternat Med (2014)

Cytotoxicity on splenocytes and inhibition on ConA-induced splenocyte proliferation of five procyanidin compounds 1–5. (a) Cytotoxicity of procyanidin compounds on BALB/c mice splenocytes. The cells were incubated with different concentration of procyanidin compounds for 48 h. And the cell viability was measured by MTT assay. (b) Inhibition of procyanidin compounds on ConA-induced splenocyte proliferation. BALB/c mice splenocytes (4 × 105 cells/well) were stimulated by ConA (2 μg/mL) for 48 h in the presence of different procyanidin compounds. Cells were then pulsed with 0.25 μCi [3H]thymidine 8 h before the end of the experiment and were assessed for [3H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. *P < 0.05, **P < 0.01, treatment group versus control.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: Cytotoxicity on splenocytes and inhibition on ConA-induced splenocyte proliferation of five procyanidin compounds 1–5. (a) Cytotoxicity of procyanidin compounds on BALB/c mice splenocytes. The cells were incubated with different concentration of procyanidin compounds for 48 h. And the cell viability was measured by MTT assay. (b) Inhibition of procyanidin compounds on ConA-induced splenocyte proliferation. BALB/c mice splenocytes (4 × 105 cells/well) were stimulated by ConA (2 μg/mL) for 48 h in the presence of different procyanidin compounds. Cells were then pulsed with 0.25 μCi [3H]thymidine 8 h before the end of the experiment and were assessed for [3H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. *P < 0.05, **P < 0.01, treatment group versus control.
Mentions: The immunosuppressive effects of the five procyanidins were determined using the same method (Figure 4). It was found that all five test compounds have no cytotoxic effect at concentrations from 0.16 μM to 20 μM (Figure 4(a)). Figure 4(b) showed that, even at a low concentration of 0.16 μM, CTD-1 can inhibit ConA-induced splenocyte proliferation by 40%, suggesting that CTD-1 produced the best effects of all compounds. Other A-type procyanidin oligomers CTB-1 and PA-1 significantly suppressed ConA-induced splenocyte proliferation at 20 μM. However, B-type procyanidin dimer PB-2 had no inhibitory effect even at 20 μM. Meanwhile, B-type procyanidin trimer PC-1 had weakly effect on ConA-induced splenocyte proliferation, only reducing it by 37% at a high concentration of 20 μM.

Bottom Line: Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear.Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1.CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine (SHUTCM), Shanghai 201203, China.

ABSTRACT
Cinnamon barks extracts have been reported to regulate immune function; however, the component(s) in cinnamon barks responsible for this effect is/are not yet clear. The aim of this study is to find out the possible component(s) that can be used as therapeutic agents for immune-related diseases from cinnamon bark. In this study, the immunosuppressive effects of fraction (named CT-F) and five procyanidin oligomers compounds, cinnamtannin B1, cinnamtannin D1 (CTD-1), parameritannin A1, procyanidin B2, and procyanidin C1, from Cinnamomum tamala or Cinnamomum cassia bark were examined on splenocytes proliferation model induced by ConA or LPS. Then, the effects of activated compound CTD-1 on cytokine production and 2,4-dinitrofluorobenzene (DNFB) induced delayed-type hypersensitivity (DTH) response were detected to evaluate the immunosuppressive activity of CTD-1. It was found that CT-F and CTD-1 significantly inhibited the splenocyte proliferation induced by ConA or LPS. CTD-1 dose-dependently reduced the level of IFN-γ and IL-2 and intensively suppressed DNFB-induced DTH responses. These findings suggest that the immunosuppressive activities of cinnamon bark are in part due to procyanidin oligomers. CTD-1 may be a potential therapeutic agent for immune-related diseases.

No MeSH data available.


Related in: MedlinePlus