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Upk3b is dispensable for development and integrity of urothelium and mesothelium.

Rudat C, Grieskamp T, Röhr C, Airik R, Wrede C, Hegermann J, Herrmann BG, Schuster-Gossler K, Kispert A - PLoS ONE (2014)

Bottom Line: To analyze Upk3b function, we generated a creERT2 knock-in allele by homologous recombination in embryonic stem cells.We show that Upk3bcreERT2 represents a allele despite the lack of creERT2 expression from the mutated locus.Upk3b is coexpressed with the closely related Upk3a gene in the urothelium but not in the mesothelium, leaving the possibility of a functional redundancy between the two genes in the urothelium only.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology, Medizinische Hochschule Hannover, Hannover, Germany.

ABSTRACT
The mesothelium, the lining of the coelomic cavities, and the urothelium, the inner lining of the urinary drainage system, are highly specialized epithelia that protect the underlying tissues from mechanical stress and seal them from the overlying fluid space. The development of these epithelia from simple precursors and the molecular characteristics of the mature tissues are poorly analyzed. Here, we show that uroplakin 3B (Upk3b), which encodes an integral membrane protein of the tetraspanin superfamily, is specifically expressed both in development as well as under homeostatic conditions in adult mice in the mesothelia of the body cavities, i.e., the epicardium and pericardium, the pleura and the peritoneum, and in the urothelium of the urinary tract. To analyze Upk3b function, we generated a creERT2 knock-in allele by homologous recombination in embryonic stem cells. We show that Upk3bcreERT2 represents a allele despite the lack of creERT2 expression from the mutated locus. Morphological, histological and molecular analyses of Upk3b-deficient mice did not detect changes in differentiation or integrity of the urothelium and the mesothelia that cover internal organs. Upk3b is coexpressed with the closely related Upk3a gene in the urothelium but not in the mesothelium, leaving the possibility of a functional redundancy between the two genes in the urothelium only.

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Related in: MedlinePlus

Upk3b expression in embryonic development.In situ hybridization analysis of Upk3b expression in whole wildtype embryos (A, B), on sagittal embryo sections (C–J and M–O) and on transverse embryo sections (K, L). (A–E) Overview images of embryos; anterior is up, dorsal is to the right. (F–O) Higher magnification images of the regions marked by open rectangles (in A–E). Stages are as indicated. Arrows point to the epicardium. a, atrium; at, epicardial covering of the dorsal wall of the great arterial trunks; bl, urinary bladder; bw, body wall; da, dorsal aorta; fl, fore limb bud; g, gut; h, heart; ki, kidney; li, liver; lu, lung; nd, nephric duct; nt, neural tube; ur, urogenital ridge; pe, pericardium of the dorsal wall of the pericardial coelom; peo, proepicardium; ppm, pleuropericardial membrane; v, ventricle.
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pone-0112112-g001: Upk3b expression in embryonic development.In situ hybridization analysis of Upk3b expression in whole wildtype embryos (A, B), on sagittal embryo sections (C–J and M–O) and on transverse embryo sections (K, L). (A–E) Overview images of embryos; anterior is up, dorsal is to the right. (F–O) Higher magnification images of the regions marked by open rectangles (in A–E). Stages are as indicated. Arrows point to the epicardium. a, atrium; at, epicardial covering of the dorsal wall of the great arterial trunks; bl, urinary bladder; bw, body wall; da, dorsal aorta; fl, fore limb bud; g, gut; h, heart; ki, kidney; li, liver; lu, lung; nd, nephric duct; nt, neural tube; ur, urogenital ridge; pe, pericardium of the dorsal wall of the pericardial coelom; peo, proepicardium; ppm, pleuropericardial membrane; v, ventricle.

Mentions: To determine the expression pattern of Upk3b during embryonic development, we performed mRNA in situ hybridization analysis of whole embryos at E9.5 and E10.5 and of sections of E9.5 to E16.5 embryos (Figure 1). In E9.5 embryos expression of Upk3b was detected in the epithelial lining of the peritoneal cavity both in the parietal layer of the body wall and the visceral layer covering the urogenital ridge and the gut tube, in the proepicardium, in the pericardium of the dorsal wall of the pericardial coelom, and in single epicardial cells that were attached to the ventricular myocardium at this stage (Figure 1A, F and K). At E10.5, Upk3b was expressed in the contiguous epicardium in addition to all mesothelia of the peritoneal cavity (Figure 1B, G and L). At E12.5 and subsequent embryonic stages, all mesothelia (i.e. epicardium and pericardium, pleura, and peritoneum) expressed Upk3b. From E14.5 on, the epithelial lining of the developing urinary tract including the renal pelvis, the lumen of the bladder and the ureter was positive for Upk3b expression as well (Figure 1D–E, I–J and N–O).


Upk3b is dispensable for development and integrity of urothelium and mesothelium.

Rudat C, Grieskamp T, Röhr C, Airik R, Wrede C, Hegermann J, Herrmann BG, Schuster-Gossler K, Kispert A - PLoS ONE (2014)

Upk3b expression in embryonic development.In situ hybridization analysis of Upk3b expression in whole wildtype embryos (A, B), on sagittal embryo sections (C–J and M–O) and on transverse embryo sections (K, L). (A–E) Overview images of embryos; anterior is up, dorsal is to the right. (F–O) Higher magnification images of the regions marked by open rectangles (in A–E). Stages are as indicated. Arrows point to the epicardium. a, atrium; at, epicardial covering of the dorsal wall of the great arterial trunks; bl, urinary bladder; bw, body wall; da, dorsal aorta; fl, fore limb bud; g, gut; h, heart; ki, kidney; li, liver; lu, lung; nd, nephric duct; nt, neural tube; ur, urogenital ridge; pe, pericardium of the dorsal wall of the pericardial coelom; peo, proepicardium; ppm, pleuropericardial membrane; v, ventricle.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4229118&req=5

pone-0112112-g001: Upk3b expression in embryonic development.In situ hybridization analysis of Upk3b expression in whole wildtype embryos (A, B), on sagittal embryo sections (C–J and M–O) and on transverse embryo sections (K, L). (A–E) Overview images of embryos; anterior is up, dorsal is to the right. (F–O) Higher magnification images of the regions marked by open rectangles (in A–E). Stages are as indicated. Arrows point to the epicardium. a, atrium; at, epicardial covering of the dorsal wall of the great arterial trunks; bl, urinary bladder; bw, body wall; da, dorsal aorta; fl, fore limb bud; g, gut; h, heart; ki, kidney; li, liver; lu, lung; nd, nephric duct; nt, neural tube; ur, urogenital ridge; pe, pericardium of the dorsal wall of the pericardial coelom; peo, proepicardium; ppm, pleuropericardial membrane; v, ventricle.
Mentions: To determine the expression pattern of Upk3b during embryonic development, we performed mRNA in situ hybridization analysis of whole embryos at E9.5 and E10.5 and of sections of E9.5 to E16.5 embryos (Figure 1). In E9.5 embryos expression of Upk3b was detected in the epithelial lining of the peritoneal cavity both in the parietal layer of the body wall and the visceral layer covering the urogenital ridge and the gut tube, in the proepicardium, in the pericardium of the dorsal wall of the pericardial coelom, and in single epicardial cells that were attached to the ventricular myocardium at this stage (Figure 1A, F and K). At E10.5, Upk3b was expressed in the contiguous epicardium in addition to all mesothelia of the peritoneal cavity (Figure 1B, G and L). At E12.5 and subsequent embryonic stages, all mesothelia (i.e. epicardium and pericardium, pleura, and peritoneum) expressed Upk3b. From E14.5 on, the epithelial lining of the developing urinary tract including the renal pelvis, the lumen of the bladder and the ureter was positive for Upk3b expression as well (Figure 1D–E, I–J and N–O).

Bottom Line: To analyze Upk3b function, we generated a creERT2 knock-in allele by homologous recombination in embryonic stem cells.We show that Upk3bcreERT2 represents a allele despite the lack of creERT2 expression from the mutated locus.Upk3b is coexpressed with the closely related Upk3a gene in the urothelium but not in the mesothelium, leaving the possibility of a functional redundancy between the two genes in the urothelium only.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology, Medizinische Hochschule Hannover, Hannover, Germany.

ABSTRACT
The mesothelium, the lining of the coelomic cavities, and the urothelium, the inner lining of the urinary drainage system, are highly specialized epithelia that protect the underlying tissues from mechanical stress and seal them from the overlying fluid space. The development of these epithelia from simple precursors and the molecular characteristics of the mature tissues are poorly analyzed. Here, we show that uroplakin 3B (Upk3b), which encodes an integral membrane protein of the tetraspanin superfamily, is specifically expressed both in development as well as under homeostatic conditions in adult mice in the mesothelia of the body cavities, i.e., the epicardium and pericardium, the pleura and the peritoneum, and in the urothelium of the urinary tract. To analyze Upk3b function, we generated a creERT2 knock-in allele by homologous recombination in embryonic stem cells. We show that Upk3bcreERT2 represents a allele despite the lack of creERT2 expression from the mutated locus. Morphological, histological and molecular analyses of Upk3b-deficient mice did not detect changes in differentiation or integrity of the urothelium and the mesothelia that cover internal organs. Upk3b is coexpressed with the closely related Upk3a gene in the urothelium but not in the mesothelium, leaving the possibility of a functional redundancy between the two genes in the urothelium only.

Show MeSH
Related in: MedlinePlus