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Chemopreventive efficacy of Andrographis paniculata on azoxymethane-induced aberrant colon crypt foci in vivo.

Al-Henhena N, Ying RP, Ismail S, Najm W, Najm W, Khalifa SA, El-Seedi H, Ameen Abdulla M, Abdulla MA - PLoS ONE (2014)

Bottom Line: Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups.Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and β-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group.When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
Andrographis paniculata is a grass-shaped medicinal herb, traditionally used in Southeast Asia. The aim of this study was to evaluate the chemoprotective effects of A. paniculata on colorectal cancer. A. paniculata ethanol extract was tested on azoxymethane (AOM)-induced aberrant crypt foci (ACF) in vivo and in vitro. A. paniculata treated groups showed a significant reduction in the number of ACF of the treated rats. Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups. Histologically, staining showed slightly elevated masses above the surrounding mucosa with oval or slit-like orifices. Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and β-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group. When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group. A. paniculata ethanol extract showed antioxidant and free radical scavenging activity, as elucidated by the measure of oxidative stress markers. Further, the active fractions were assessed against cell lines of CCD841 and HT29 colon cancer cells.

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(a): Regulation of PCNA in the colon tissue of rats (A) Normal group (B) AOM control group (C) FU treated group, (D) 250 mg/kg A. paniculata treated group, and (E) 500 mg/kg A. paniculata treated group (100X magnification). (b): Regulation of PCNA protein expression in the colon tissue of normal and AOM induced rats. All values are in mean ± SEM. FU: Fluorouracil; AOM: azoxymethane.
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pone-0111118-g005: (a): Regulation of PCNA in the colon tissue of rats (A) Normal group (B) AOM control group (C) FU treated group, (D) 250 mg/kg A. paniculata treated group, and (E) 500 mg/kg A. paniculata treated group (100X magnification). (b): Regulation of PCNA protein expression in the colon tissue of normal and AOM induced rats. All values are in mean ± SEM. FU: Fluorouracil; AOM: azoxymethane.

Mentions: AOM group had high expressions of PCNA and β-catenin proteins (Figures 5, 6) compared to the other three groups based on the immunohistochemical appearance. The sections of the AOM group showed the brownish positive colour revealing the up-regulation of PCNA protein, in contrast to the treated groups where the bluish appearance indicated PCNA protein down-regulation. Overexpression of beta catenin protein in AOM control group was observed with respect to treated groups.


Chemopreventive efficacy of Andrographis paniculata on azoxymethane-induced aberrant colon crypt foci in vivo.

Al-Henhena N, Ying RP, Ismail S, Najm W, Najm W, Khalifa SA, El-Seedi H, Ameen Abdulla M, Abdulla MA - PLoS ONE (2014)

(a): Regulation of PCNA in the colon tissue of rats (A) Normal group (B) AOM control group (C) FU treated group, (D) 250 mg/kg A. paniculata treated group, and (E) 500 mg/kg A. paniculata treated group (100X magnification). (b): Regulation of PCNA protein expression in the colon tissue of normal and AOM induced rats. All values are in mean ± SEM. FU: Fluorouracil; AOM: azoxymethane.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4229078&req=5

pone-0111118-g005: (a): Regulation of PCNA in the colon tissue of rats (A) Normal group (B) AOM control group (C) FU treated group, (D) 250 mg/kg A. paniculata treated group, and (E) 500 mg/kg A. paniculata treated group (100X magnification). (b): Regulation of PCNA protein expression in the colon tissue of normal and AOM induced rats. All values are in mean ± SEM. FU: Fluorouracil; AOM: azoxymethane.
Mentions: AOM group had high expressions of PCNA and β-catenin proteins (Figures 5, 6) compared to the other three groups based on the immunohistochemical appearance. The sections of the AOM group showed the brownish positive colour revealing the up-regulation of PCNA protein, in contrast to the treated groups where the bluish appearance indicated PCNA protein down-regulation. Overexpression of beta catenin protein in AOM control group was observed with respect to treated groups.

Bottom Line: Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups.Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and β-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group.When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
Andrographis paniculata is a grass-shaped medicinal herb, traditionally used in Southeast Asia. The aim of this study was to evaluate the chemoprotective effects of A. paniculata on colorectal cancer. A. paniculata ethanol extract was tested on azoxymethane (AOM)-induced aberrant crypt foci (ACF) in vivo and in vitro. A. paniculata treated groups showed a significant reduction in the number of ACF of the treated rats. Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups. Histologically, staining showed slightly elevated masses above the surrounding mucosa with oval or slit-like orifices. Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and β-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group. When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group. A. paniculata ethanol extract showed antioxidant and free radical scavenging activity, as elucidated by the measure of oxidative stress markers. Further, the active fractions were assessed against cell lines of CCD841 and HT29 colon cancer cells.

Show MeSH
Related in: MedlinePlus