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Chemopreventive efficacy of Andrographis paniculata on azoxymethane-induced aberrant colon crypt foci in vivo.

Al-Henhena N, Ying RP, Ismail S, Najm W, Najm W, Khalifa SA, El-Seedi H, Ameen Abdulla M, Abdulla MA - PLoS ONE (2014)

Bottom Line: Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups.Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and β-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group.When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
Andrographis paniculata is a grass-shaped medicinal herb, traditionally used in Southeast Asia. The aim of this study was to evaluate the chemoprotective effects of A. paniculata on colorectal cancer. A. paniculata ethanol extract was tested on azoxymethane (AOM)-induced aberrant crypt foci (ACF) in vivo and in vitro. A. paniculata treated groups showed a significant reduction in the number of ACF of the treated rats. Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups. Histologically, staining showed slightly elevated masses above the surrounding mucosa with oval or slit-like orifices. Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and β-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group. When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group. A. paniculata ethanol extract showed antioxidant and free radical scavenging activity, as elucidated by the measure of oxidative stress markers. Further, the active fractions were assessed against cell lines of CCD841 and HT29 colon cancer cells.

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Effect of A. paniculata on superoxide dismutase (SOD) activity, malonaldehyde (MDA) level and nitric oxide (NO) level in AOM induced tissue.All values are represented as mean ± SEM. Significant difference at *P<0.01, ** P<0.001(ANOVA, Tukey's). FU: Fluorouracil; AOM: azoxymethane.
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pone-0111118-g002: Effect of A. paniculata on superoxide dismutase (SOD) activity, malonaldehyde (MDA) level and nitric oxide (NO) level in AOM induced tissue.All values are represented as mean ± SEM. Significant difference at *P<0.01, ** P<0.001(ANOVA, Tukey's). FU: Fluorouracil; AOM: azoxymethane.

Mentions: Malondialdehyde (MDA), nitric oxide (NO) levels and superoxide dismutase (SOD) activity of the colon homogenate differed among groups. Malondialdehyde levels were significantly lower in A. paniculata (4.7±0.33, 4.1±0.46 at 250 mg/kg, 500 mg/kg, respectively) and FU treated rats (3.6±0.12 µm) (Figure 2). Likewise, nitric oxide levels were significantly lower in A. paniculata treated rats with a value of 10.9±0.32 µm at 250 mg/kg and 8.2±1.7 µm at 500 mg/kg (Figure 2). Moreover, SOD activity showed values of 11.5±0.9 and 11.1±0.2 U/ml at dosages of 250 and 500 mg/kg (Figure 2).


Chemopreventive efficacy of Andrographis paniculata on azoxymethane-induced aberrant colon crypt foci in vivo.

Al-Henhena N, Ying RP, Ismail S, Najm W, Najm W, Khalifa SA, El-Seedi H, Ameen Abdulla M, Abdulla MA - PLoS ONE (2014)

Effect of A. paniculata on superoxide dismutase (SOD) activity, malonaldehyde (MDA) level and nitric oxide (NO) level in AOM induced tissue.All values are represented as mean ± SEM. Significant difference at *P<0.01, ** P<0.001(ANOVA, Tukey's). FU: Fluorouracil; AOM: azoxymethane.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4229078&req=5

pone-0111118-g002: Effect of A. paniculata on superoxide dismutase (SOD) activity, malonaldehyde (MDA) level and nitric oxide (NO) level in AOM induced tissue.All values are represented as mean ± SEM. Significant difference at *P<0.01, ** P<0.001(ANOVA, Tukey's). FU: Fluorouracil; AOM: azoxymethane.
Mentions: Malondialdehyde (MDA), nitric oxide (NO) levels and superoxide dismutase (SOD) activity of the colon homogenate differed among groups. Malondialdehyde levels were significantly lower in A. paniculata (4.7±0.33, 4.1±0.46 at 250 mg/kg, 500 mg/kg, respectively) and FU treated rats (3.6±0.12 µm) (Figure 2). Likewise, nitric oxide levels were significantly lower in A. paniculata treated rats with a value of 10.9±0.32 µm at 250 mg/kg and 8.2±1.7 µm at 500 mg/kg (Figure 2). Moreover, SOD activity showed values of 11.5±0.9 and 11.1±0.2 U/ml at dosages of 250 and 500 mg/kg (Figure 2).

Bottom Line: Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups.Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and β-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group.When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
Andrographis paniculata is a grass-shaped medicinal herb, traditionally used in Southeast Asia. The aim of this study was to evaluate the chemoprotective effects of A. paniculata on colorectal cancer. A. paniculata ethanol extract was tested on azoxymethane (AOM)-induced aberrant crypt foci (ACF) in vivo and in vitro. A. paniculata treated groups showed a significant reduction in the number of ACF of the treated rats. Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups. Histologically, staining showed slightly elevated masses above the surrounding mucosa with oval or slit-like orifices. Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and β-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group. When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group. A. paniculata ethanol extract showed antioxidant and free radical scavenging activity, as elucidated by the measure of oxidative stress markers. Further, the active fractions were assessed against cell lines of CCD841 and HT29 colon cancer cells.

Show MeSH
Related in: MedlinePlus