Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance.
Bottom Line: Bemisia tabaci has developed high levels of resistance to many insecticides including the neonicotinoids and there is strong evidence that for some compounds resistance is stage-specific.Gene ontology and bioinformatic analyses revealed that in all life stages many of the differentially expressed transcripts encoded enzymes involved in metabolic processes and/or metabolism of xenobiotics.Here, we evaluated both common and stage-specific gene expression signatures and identified several candidate resistance genes that may underlie B. tabaci resistance to thiamethoxam.
Affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.Show MeSH
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Mentions: Changes in global gene expression in different development stages of the thiamethoxam selected strain (TH-R) were compared with the equivalent life stage of an unselected culture of the same strain (TH-S) using a custom Agilent microarray. In total, 1043 ESTs were identified as significantly differentially expressed in at least one of the three life stages examined with each development stage displaying a distinct gene expression signature (Figs 1A, 1A, Table S3). Amongst these, only ∼4% (37 ESTs) were differentially expressed in all three stages (Table 2) with just 10 ESTs over-expressed. Approximately 22% (230 ESTs) of the differentially expressed genes were common to two stages and ∼74% (776 genes) were unique to just one stage (Fig. 2B). The expression of 434 ESTs was altered in the egg stage, amongst which ∼59% were up-regulated. The nymph stage had 553 differentially expressed ESTs with ∼42% of them up-regulated. Finally in the female adult stage, 360 ESTs were differentially expressed with ∼61% over-expressed.
Affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.