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Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance.

Yang N, Xie W, Jones CM, Bass C, Jiao X, Yang X, Liu B, Li R, Zhang Y - Insect Mol. Biol. (2013)

Bottom Line: Gene ontology and bioinformatic analyses revealed that in all life stages many of the differentially expressed transcripts encoded enzymes involved in metabolic processes and/or metabolism of xenobiotics.Several of these are candidate resistance genes and include the cytochrome P450 CYP6CM1, which has been shown to confer resistance to several neonicotinoids previously, a P450 belonging to the Cytochrome P450s 4 family and a glutathione S-transferase (GST) belonging to the sigma class.Here, we evaluated both common and stage-specific gene expression signatures and identified several candidate resistance genes that may underlie B. tabaci resistance to thiamethoxam.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.

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Stage-specific gene expression signatures in Bemisia tabaci. (A) Hierarchical cluster analysis of altered expressed sequence tags (ESTs) (/log2 ratio/ ≥ 1, P < 0.05) at the three stages in B. tabaci between thiamethoxam resistance and susceptible were characterized as the following: nymph stage in thiamethoxam resistant (TH-R; S1) and TH susceptible (TH-S; S2), respectively; egg stage in TH-R (S3) and TH-S (S4), respectively; female adult stage in TH-R (S5) and TH-S (S6), respectively. Green, red and black areas indicate, respectively, decreased, increased and no significant change in thiamethoxam resistance compared with susceptible. (B) Venn diagram depicting the number of ESTs with altered expression (/log2 ratio/ ≥ 1, P < 0.05) at three stages between thiamethoxam resistance and susceptible, respectively. The size of each region is proportional to the number of altered ESTs.
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fig02: Stage-specific gene expression signatures in Bemisia tabaci. (A) Hierarchical cluster analysis of altered expressed sequence tags (ESTs) (/log2 ratio/ ≥ 1, P < 0.05) at the three stages in B. tabaci between thiamethoxam resistance and susceptible were characterized as the following: nymph stage in thiamethoxam resistant (TH-R; S1) and TH susceptible (TH-S; S2), respectively; egg stage in TH-R (S3) and TH-S (S4), respectively; female adult stage in TH-R (S5) and TH-S (S6), respectively. Green, red and black areas indicate, respectively, decreased, increased and no significant change in thiamethoxam resistance compared with susceptible. (B) Venn diagram depicting the number of ESTs with altered expression (/log2 ratio/ ≥ 1, P < 0.05) at three stages between thiamethoxam resistance and susceptible, respectively. The size of each region is proportional to the number of altered ESTs.

Mentions: Changes in global gene expression in different development stages of the thiamethoxam selected strain (TH-R) were compared with the equivalent life stage of an unselected culture of the same strain (TH-S) using a custom Agilent microarray. In total, 1043 ESTs were identified as significantly differentially expressed in at least one of the three life stages examined with each development stage displaying a distinct gene expression signature (Figs 1A, 1A, Table S3). Amongst these, only ∼4% (37 ESTs) were differentially expressed in all three stages (Table 2) with just 10 ESTs over-expressed. Approximately 22% (230 ESTs) of the differentially expressed genes were common to two stages and ∼74% (776 genes) were unique to just one stage (Fig. 2B). The expression of 434 ESTs was altered in the egg stage, amongst which ∼59% were up-regulated. The nymph stage had 553 differentially expressed ESTs with ∼42% of them up-regulated. Finally in the female adult stage, 360 ESTs were differentially expressed with ∼61% over-expressed.


Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance.

Yang N, Xie W, Jones CM, Bass C, Jiao X, Yang X, Liu B, Li R, Zhang Y - Insect Mol. Biol. (2013)

Stage-specific gene expression signatures in Bemisia tabaci. (A) Hierarchical cluster analysis of altered expressed sequence tags (ESTs) (/log2 ratio/ ≥ 1, P < 0.05) at the three stages in B. tabaci between thiamethoxam resistance and susceptible were characterized as the following: nymph stage in thiamethoxam resistant (TH-R; S1) and TH susceptible (TH-S; S2), respectively; egg stage in TH-R (S3) and TH-S (S4), respectively; female adult stage in TH-R (S5) and TH-S (S6), respectively. Green, red and black areas indicate, respectively, decreased, increased and no significant change in thiamethoxam resistance compared with susceptible. (B) Venn diagram depicting the number of ESTs with altered expression (/log2 ratio/ ≥ 1, P < 0.05) at three stages between thiamethoxam resistance and susceptible, respectively. The size of each region is proportional to the number of altered ESTs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4229068&req=5

fig02: Stage-specific gene expression signatures in Bemisia tabaci. (A) Hierarchical cluster analysis of altered expressed sequence tags (ESTs) (/log2 ratio/ ≥ 1, P < 0.05) at the three stages in B. tabaci between thiamethoxam resistance and susceptible were characterized as the following: nymph stage in thiamethoxam resistant (TH-R; S1) and TH susceptible (TH-S; S2), respectively; egg stage in TH-R (S3) and TH-S (S4), respectively; female adult stage in TH-R (S5) and TH-S (S6), respectively. Green, red and black areas indicate, respectively, decreased, increased and no significant change in thiamethoxam resistance compared with susceptible. (B) Venn diagram depicting the number of ESTs with altered expression (/log2 ratio/ ≥ 1, P < 0.05) at three stages between thiamethoxam resistance and susceptible, respectively. The size of each region is proportional to the number of altered ESTs.
Mentions: Changes in global gene expression in different development stages of the thiamethoxam selected strain (TH-R) were compared with the equivalent life stage of an unselected culture of the same strain (TH-S) using a custom Agilent microarray. In total, 1043 ESTs were identified as significantly differentially expressed in at least one of the three life stages examined with each development stage displaying a distinct gene expression signature (Figs 1A, 1A, Table S3). Amongst these, only ∼4% (37 ESTs) were differentially expressed in all three stages (Table 2) with just 10 ESTs over-expressed. Approximately 22% (230 ESTs) of the differentially expressed genes were common to two stages and ∼74% (776 genes) were unique to just one stage (Fig. 2B). The expression of 434 ESTs was altered in the egg stage, amongst which ∼59% were up-regulated. The nymph stage had 553 differentially expressed ESTs with ∼42% of them up-regulated. Finally in the female adult stage, 360 ESTs were differentially expressed with ∼61% over-expressed.

Bottom Line: Gene ontology and bioinformatic analyses revealed that in all life stages many of the differentially expressed transcripts encoded enzymes involved in metabolic processes and/or metabolism of xenobiotics.Several of these are candidate resistance genes and include the cytochrome P450 CYP6CM1, which has been shown to confer resistance to several neonicotinoids previously, a P450 belonging to the Cytochrome P450s 4 family and a glutathione S-transferase (GST) belonging to the sigma class.Here, we evaluated both common and stage-specific gene expression signatures and identified several candidate resistance genes that may underlie B. tabaci resistance to thiamethoxam.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.

Show MeSH
Related in: MedlinePlus