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MADS-box transcription factor AGL21 regulates lateral root development and responds to multiple external and physiological signals.

Yu LH, Miao ZQ, Qi GF, Wu J, Cai XT, Mao JL, Xiang CB - Mol Plant (2014)

Bottom Line: AGL21 was induced by many plant hormones and environmental stresses, suggesting a function of this gene in root system plasticity in response to various signals.Furthermore, AGL21 was found positively regulating auxin accumulation in lateral root primordia and lateral roots by enhancing local auxin biosynthesis, thus stimulating lateral root initiation and growth.We propose that AGL21 may be involved in various environmental and physiological signals-mediated lateral root development and growth.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui Province 230027, China.

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AGL21 Regulates Auxin Accumulation in the LRPs and LRs.(A) Quantification of free IAA content in the root of 9-day-old plants. Values are mean ± SD of three replica experiments and asterisks denote Student’s t-test significance compared with the wild-type plants: * P < 0.05; ** P < 0.01.(B–F) Expression of DR5:GUS (N = 20 plants) in three different stages of LRP (B–D), LR, and PR tips (E, F) of Col-0 plants. Nine-day-old plants grown on MS medium were used for GUS staining for 6h.(G–K) Expression of DR5:GUS in three different stages of LRP (G–I), LR, and PR tips (J, K) of agl21 knockout plants.(L–P) Expression of DR5:GUS in three different stages of LRP (L–N), LR, and PR tips (O, P) of AGL21-overexpressing plants.
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Figure 8: AGL21 Regulates Auxin Accumulation in the LRPs and LRs.(A) Quantification of free IAA content in the root of 9-day-old plants. Values are mean ± SD of three replica experiments and asterisks denote Student’s t-test significance compared with the wild-type plants: * P < 0.05; ** P < 0.01.(B–F) Expression of DR5:GUS (N = 20 plants) in three different stages of LRP (B–D), LR, and PR tips (E, F) of Col-0 plants. Nine-day-old plants grown on MS medium were used for GUS staining for 6h.(G–K) Expression of DR5:GUS in three different stages of LRP (G–I), LR, and PR tips (J, K) of agl21 knockout plants.(L–P) Expression of DR5:GUS in three different stages of LRP (L–N), LR, and PR tips (O, P) of AGL21-overexpressing plants.

Mentions: The LR phenotypes of agl21 mutant and the overexpression lines implicate that root auxin level might be affected. To verify whether endogenous auxin content was changed, we measured endogenous IAA in the root. The results in Figure 8A show that IAA content was significantly increased in the overexpression line and reduced in the knockout mutant compared with that in the wild-type.


MADS-box transcription factor AGL21 regulates lateral root development and responds to multiple external and physiological signals.

Yu LH, Miao ZQ, Qi GF, Wu J, Cai XT, Mao JL, Xiang CB - Mol Plant (2014)

AGL21 Regulates Auxin Accumulation in the LRPs and LRs.(A) Quantification of free IAA content in the root of 9-day-old plants. Values are mean ± SD of three replica experiments and asterisks denote Student’s t-test significance compared with the wild-type plants: * P < 0.05; ** P < 0.01.(B–F) Expression of DR5:GUS (N = 20 plants) in three different stages of LRP (B–D), LR, and PR tips (E, F) of Col-0 plants. Nine-day-old plants grown on MS medium were used for GUS staining for 6h.(G–K) Expression of DR5:GUS in three different stages of LRP (G–I), LR, and PR tips (J, K) of agl21 knockout plants.(L–P) Expression of DR5:GUS in three different stages of LRP (L–N), LR, and PR tips (O, P) of AGL21-overexpressing plants.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4228986&req=5

Figure 8: AGL21 Regulates Auxin Accumulation in the LRPs and LRs.(A) Quantification of free IAA content in the root of 9-day-old plants. Values are mean ± SD of three replica experiments and asterisks denote Student’s t-test significance compared with the wild-type plants: * P < 0.05; ** P < 0.01.(B–F) Expression of DR5:GUS (N = 20 plants) in three different stages of LRP (B–D), LR, and PR tips (E, F) of Col-0 plants. Nine-day-old plants grown on MS medium were used for GUS staining for 6h.(G–K) Expression of DR5:GUS in three different stages of LRP (G–I), LR, and PR tips (J, K) of agl21 knockout plants.(L–P) Expression of DR5:GUS in three different stages of LRP (L–N), LR, and PR tips (O, P) of AGL21-overexpressing plants.
Mentions: The LR phenotypes of agl21 mutant and the overexpression lines implicate that root auxin level might be affected. To verify whether endogenous auxin content was changed, we measured endogenous IAA in the root. The results in Figure 8A show that IAA content was significantly increased in the overexpression line and reduced in the knockout mutant compared with that in the wild-type.

Bottom Line: AGL21 was induced by many plant hormones and environmental stresses, suggesting a function of this gene in root system plasticity in response to various signals.Furthermore, AGL21 was found positively regulating auxin accumulation in lateral root primordia and lateral roots by enhancing local auxin biosynthesis, thus stimulating lateral root initiation and growth.We propose that AGL21 may be involved in various environmental and physiological signals-mediated lateral root development and growth.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui Province 230027, China.

Show MeSH
Related in: MedlinePlus