Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis.
Bottom Line: Moreover, cotyledons of mpk10 and mkk2 mutants have reduced vein complexity, which can be reversed by inhibiting polar auxin transport (PAT).Auxin does not affect AtMPK10 expression while treatment with the PAT inhibitor HFCA extends the expression in leaves and reverses the mpk10 mutant phenotype.These results suggest that the AtMKK2-AtMPK10 MAPK module regulates venation complexity by altering PAT efficiency.
Affiliation: Department of Plant Molecular Biology, Max. F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9/4, Vienna, A-1030, Austria Present address: Felix-Klein-Gymnasium, Böttingerstraße 17, D-37073 Göttingen, Germany.Show MeSH
Related in: MedlinePlus
License 1 - License 2
Mentions: These results pointed to a possible role of the AtMKK2/AtMPK10 kinase cascade in PAT controlling vascular development (Figure 6). As treatment of the mpk10 mutant with PAT inhibitors had the same effect as expression of AtMPK10 or AtMKK2 in this mutant background, it seems that the AtMKK2/AtMPK10 module plays a role in the action of PAT inhibitors. PAT inhibitors such as HFCA, NPA, or TIBA are synthetic substances but it is widely accepted that they mimic the action of natural PAT inhibitors (Murphy et al., 2000).
Affiliation: Department of Plant Molecular Biology, Max. F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9/4, Vienna, A-1030, Austria Present address: Felix-Klein-Gymnasium, Böttingerstraße 17, D-37073 Göttingen, Germany.