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Preliminary study of quercetin affecting the hypothalamic-pituitary-gonadal axis on rat endometriosis model.

Cao Y, Zhuang MF, Yang Y, Xie SW, Cui JG, Cao L, Zhang TT, Zhu Y - Evid Based Complement Alternat Med (2014)

Bottom Line: The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot.The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy.Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
In this study, the endometriosis rats model was randomly divided into 6 groups: model control group, ovariectomized group, Gestrinone group, and quercetin high/medium/low dose group. Rats were killed after 3 weeks of administration. The expression levels of serum FSH and LH were detected by ELISA. The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot. The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy. Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

No MeSH data available.


Related in: MedlinePlus

The expression of ERα in eutopic endometrium (DAB stain, ×400) ((a) model control group, (b) ovariectomized group, (c) gestrinone group, (d) quercetin low dose group, (e) quercetin medium dose group, and (f) quercetin high dose group).
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fig6: The expression of ERα in eutopic endometrium (DAB stain, ×400) ((a) model control group, (b) ovariectomized group, (c) gestrinone group, (d) quercetin low dose group, (e) quercetin medium dose group, and (f) quercetin high dose group).

Mentions: Results of immunohistochemistry showed that ERα, ERβ, and PR expressed in uterine gland epithelial cells, luminal epithelial cells, and mesenchymal cells, with glandular epithelial cells expressed as strong. Due to the decline of ovarian hormone level, atrophy of the endometrium, and reduction of estrogen and progesterone receptor content, the IOD of ovariectomized group was significantly decreased in comparison with model control group (P < 0.01). Gestrinone can obviously increase the expression of ERα and PR (P < 0.05). Quercetin high dose group can downregulate the expression of ERα and PR and, meanwhile, upregulate the expression of ERβ compared to model control group (P < 0.05) (Table 4 and Figures 6, 7, and 8).


Preliminary study of quercetin affecting the hypothalamic-pituitary-gonadal axis on rat endometriosis model.

Cao Y, Zhuang MF, Yang Y, Xie SW, Cui JG, Cao L, Zhang TT, Zhu Y - Evid Based Complement Alternat Med (2014)

The expression of ERα in eutopic endometrium (DAB stain, ×400) ((a) model control group, (b) ovariectomized group, (c) gestrinone group, (d) quercetin low dose group, (e) quercetin medium dose group, and (f) quercetin high dose group).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4228827&req=5

fig6: The expression of ERα in eutopic endometrium (DAB stain, ×400) ((a) model control group, (b) ovariectomized group, (c) gestrinone group, (d) quercetin low dose group, (e) quercetin medium dose group, and (f) quercetin high dose group).
Mentions: Results of immunohistochemistry showed that ERα, ERβ, and PR expressed in uterine gland epithelial cells, luminal epithelial cells, and mesenchymal cells, with glandular epithelial cells expressed as strong. Due to the decline of ovarian hormone level, atrophy of the endometrium, and reduction of estrogen and progesterone receptor content, the IOD of ovariectomized group was significantly decreased in comparison with model control group (P < 0.01). Gestrinone can obviously increase the expression of ERα and PR (P < 0.05). Quercetin high dose group can downregulate the expression of ERα and PR and, meanwhile, upregulate the expression of ERβ compared to model control group (P < 0.05) (Table 4 and Figures 6, 7, and 8).

Bottom Line: The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot.The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy.Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
In this study, the endometriosis rats model was randomly divided into 6 groups: model control group, ovariectomized group, Gestrinone group, and quercetin high/medium/low dose group. Rats were killed after 3 weeks of administration. The expression levels of serum FSH and LH were detected by ELISA. The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot. The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy. Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

No MeSH data available.


Related in: MedlinePlus