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Preliminary study of quercetin affecting the hypothalamic-pituitary-gonadal axis on rat endometriosis model.

Cao Y, Zhuang MF, Yang Y, Xie SW, Cui JG, Cao L, Zhang TT, Zhu Y - Evid Based Complement Alternat Med (2014)

Bottom Line: The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot.The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy.Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
In this study, the endometriosis rats model was randomly divided into 6 groups: model control group, ovariectomized group, Gestrinone group, and quercetin high/medium/low dose group. Rats were killed after 3 weeks of administration. The expression levels of serum FSH and LH were detected by ELISA. The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot. The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy. Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

No MeSH data available.


Related in: MedlinePlus

The expression of ERα in hypothalamus (DAB stain, ×400) ((a) model control group, (b) ovariectomized group, (c) gestrinone group, (d) quercetin low dose group, (e) quercetin medium dose group, and (f) quercetin high dose group).
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fig3: The expression of ERα in hypothalamus (DAB stain, ×400) ((a) model control group, (b) ovariectomized group, (c) gestrinone group, (d) quercetin low dose group, (e) quercetin medium dose group, and (f) quercetin high dose group).

Mentions: Results of immunohistochemistry showed that the integrated optical density (IOD) of ERα in model control group was significantly higher than ovariectomized group (P < 0.01). The IOD of ERα in quercetin low and medium dose group displayed no obvious difference in comparison with model control group (P > 0.05), and the IOD of ERα in quercetin high dose group was significantly reduced compared with model control group (P < 0.05). In addition to ovariectomized group, there was no obvious difference on the IOD of ERβ in the other groups compared with model control group (P > 0.05). The IOD of PR was decreased in each group in comparison with model control group except for quercetin low dose group (P < 0.05) (Table 3 and Figures 3, 4, and 5).


Preliminary study of quercetin affecting the hypothalamic-pituitary-gonadal axis on rat endometriosis model.

Cao Y, Zhuang MF, Yang Y, Xie SW, Cui JG, Cao L, Zhang TT, Zhu Y - Evid Based Complement Alternat Med (2014)

The expression of ERα in hypothalamus (DAB stain, ×400) ((a) model control group, (b) ovariectomized group, (c) gestrinone group, (d) quercetin low dose group, (e) quercetin medium dose group, and (f) quercetin high dose group).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4228827&req=5

fig3: The expression of ERα in hypothalamus (DAB stain, ×400) ((a) model control group, (b) ovariectomized group, (c) gestrinone group, (d) quercetin low dose group, (e) quercetin medium dose group, and (f) quercetin high dose group).
Mentions: Results of immunohistochemistry showed that the integrated optical density (IOD) of ERα in model control group was significantly higher than ovariectomized group (P < 0.01). The IOD of ERα in quercetin low and medium dose group displayed no obvious difference in comparison with model control group (P > 0.05), and the IOD of ERα in quercetin high dose group was significantly reduced compared with model control group (P < 0.05). In addition to ovariectomized group, there was no obvious difference on the IOD of ERβ in the other groups compared with model control group (P > 0.05). The IOD of PR was decreased in each group in comparison with model control group except for quercetin low dose group (P < 0.05) (Table 3 and Figures 3, 4, and 5).

Bottom Line: The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot.The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy.Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
In this study, the endometriosis rats model was randomly divided into 6 groups: model control group, ovariectomized group, Gestrinone group, and quercetin high/medium/low dose group. Rats were killed after 3 weeks of administration. The expression levels of serum FSH and LH were detected by ELISA. The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot. The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy. Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

No MeSH data available.


Related in: MedlinePlus