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Preliminary study of quercetin affecting the hypothalamic-pituitary-gonadal axis on rat endometriosis model.

Cao Y, Zhuang MF, Yang Y, Xie SW, Cui JG, Cao L, Zhang TT, Zhu Y - Evid Based Complement Alternat Med (2014)

Bottom Line: The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot.The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy.Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
In this study, the endometriosis rats model was randomly divided into 6 groups: model control group, ovariectomized group, Gestrinone group, and quercetin high/medium/low dose group. Rats were killed after 3 weeks of administration. The expression levels of serum FSH and LH were detected by ELISA. The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot. The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy. Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

No MeSH data available.


Related in: MedlinePlus

Quercetin structure formula.
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Related In: Results  -  Collection


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fig1: Quercetin structure formula.

Mentions: Quercetin(4H-1-benzopyran-4-one,2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-f0lavone) and C15H10O7·2H2O (molecular weight 302.23, purity greater than 98%) were kind gifts from Swiss ALEXIS Biochemical Corporation. The structure of quercetin is shown in Figure 1, and its structure is similar with mammary estrogen 17b-estradiol (Figure 2), including a pair of hydroxy, the similar distance, and a phenol ring the latter one plays the decisive role in adsorbing on estrogen receptor. Gestrinone was purchased from Beijing ZiZhu Pharmaceutical (Beijing, China). Rat FSH and LH elisa Kits were purchased from USA Rapidbio Corporation. The mouse anti-ERa monoclonal antibody (68 kD), rabbit anti-ERβ polyclonal antibody (55 kD), and mouse anti-PR antibody [PR-AT 4.14]—ChIP Grade monoclonal antibody (99 kD) were all purchased from ABCAM company.


Preliminary study of quercetin affecting the hypothalamic-pituitary-gonadal axis on rat endometriosis model.

Cao Y, Zhuang MF, Yang Y, Xie SW, Cui JG, Cao L, Zhang TT, Zhu Y - Evid Based Complement Alternat Med (2014)

Quercetin structure formula.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4228827&req=5

fig1: Quercetin structure formula.
Mentions: Quercetin(4H-1-benzopyran-4-one,2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-f0lavone) and C15H10O7·2H2O (molecular weight 302.23, purity greater than 98%) were kind gifts from Swiss ALEXIS Biochemical Corporation. The structure of quercetin is shown in Figure 1, and its structure is similar with mammary estrogen 17b-estradiol (Figure 2), including a pair of hydroxy, the similar distance, and a phenol ring the latter one plays the decisive role in adsorbing on estrogen receptor. Gestrinone was purchased from Beijing ZiZhu Pharmaceutical (Beijing, China). Rat FSH and LH elisa Kits were purchased from USA Rapidbio Corporation. The mouse anti-ERa monoclonal antibody (68 kD), rabbit anti-ERβ polyclonal antibody (55 kD), and mouse anti-PR antibody [PR-AT 4.14]—ChIP Grade monoclonal antibody (99 kD) were all purchased from ABCAM company.

Bottom Line: The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot.The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy.Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
In this study, the endometriosis rats model was randomly divided into 6 groups: model control group, ovariectomized group, Gestrinone group, and quercetin high/medium/low dose group. Rats were killed after 3 weeks of administration. The expression levels of serum FSH and LH were detected by ELISA. The localizations and quantities of ERα, ERβ, and PR were detected by immunohistochemistry and western blot. The results showed that the mechanism of quercetin inhibiting the growth of ectopic endometrium on rat endometriosis model may be through the decreasing of serum FSH and LH levels and then reducing local estrogen content to make the ectopic endometrium atrophy. Quercetin can decrease the expression of ERα, ERβ, and PR in hypothalamus, pituitary, and endometrium, thereby inhibiting estrogen and progesterone binding to their receptors to play the role of antiestrogen and progesterone.

No MeSH data available.


Related in: MedlinePlus