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Identification of a novel lncRNA in gluteal adipose tissue and evidence for its positive effect on preadipocyte differentiation.

Divoux A, Karastergiou K, Xie H, Guo W, Perera RJ, Fried SK, Smith SR - Obesity (Silver Spring) (2014)

Bottom Line: Physiological differences in gluteal compared with abdominal subcutaneous (sc) adipocyte functions are known but the molecular basis for depot differences in adipocyte function is poorly understood.The HOTAIR gene was stably transfected into primary cultured human abdominal sc preadipocytes using a lentivirus and effects on adipogenic differentiation were analyzed.The role of this lncRNA in determining the metabolic properties of gluteal compared with abdominal adipocytes merits further study.

View Article: PubMed Central - PubMed

Affiliation: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Sanford/Burnham Medical Research Institute, Orlando, Florida, USA; Diabetes and Obesity Research Center, Sanford/Burnham Medical Research Institute at Lake Nona, Orlando, Florida, USA.

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Expression of HOTAIR exclusively in human gluteal sc adipose tissueMicroarray analysis identified HOTAIR as a gene expressed only in gluteal depot (A). HOTAIR expression by qPCR in WAT paired samples (n=35 subjects, paired t-test compared with Abd of same sex *p<0.001 **p<0.0001) (B) and in vitro during differentiation of paired abdominal (ABD) and gluteal (GLUT) human primary preadipocytes (n= 4 independent experiments, paired t-test compared with Abd of same time point *p<0.05) (C).F=female; M=male
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Figure 1: Expression of HOTAIR exclusively in human gluteal sc adipose tissueMicroarray analysis identified HOTAIR as a gene expressed only in gluteal depot (A). HOTAIR expression by qPCR in WAT paired samples (n=35 subjects, paired t-test compared with Abd of same sex *p<0.001 **p<0.0001) (B) and in vitro during differentiation of paired abdominal (ABD) and gluteal (GLUT) human primary preadipocytes (n= 4 independent experiments, paired t-test compared with Abd of same time point *p<0.05) (C).F=female; M=male

Mentions: We identified HOTAIR as a long non-coding RNA expressed in gluteal but not abdominal sc adipose tissue in both sexes (Fig. 1A). These microarray results were verified using qPCR in the same group of subjects: HOTAIR was at the detection limit in abdominal sc adipose tissue samples (CT 37–40) and expressed in all gluteal adipose tissue samples (CT 29–36, Fig. 1B). HOTAIR gene expression was similar in males and females. HOTAIR expression was highly variable and enriched >10-fold in isolated gluteal adipocytes compared to gluteal adipose tissue, but was also expressed in gluteal stromal cells (tissue levels: 4.5±4.1 arbitrary units (AU), adipocytes: 58.8±23.7, stromal-vascular fraction 13.5±10.5 AU). In contrast, HOTAIR was at the limit of detection in both cell fractions of abdominal adipose tissue. To determine whether gluteal adipocytes expressed HOTAIR in a cell autonomous, depot-specific manner, we examined expression in primary cultures of preadipocytes from both depots. The depot difference in HOTAIR gene expression is maintained ex vivo throughout differentiation. Differentiation increased HOTAIR expression by 2-fold (Fig 1C).


Identification of a novel lncRNA in gluteal adipose tissue and evidence for its positive effect on preadipocyte differentiation.

Divoux A, Karastergiou K, Xie H, Guo W, Perera RJ, Fried SK, Smith SR - Obesity (Silver Spring) (2014)

Expression of HOTAIR exclusively in human gluteal sc adipose tissueMicroarray analysis identified HOTAIR as a gene expressed only in gluteal depot (A). HOTAIR expression by qPCR in WAT paired samples (n=35 subjects, paired t-test compared with Abd of same sex *p<0.001 **p<0.0001) (B) and in vitro during differentiation of paired abdominal (ABD) and gluteal (GLUT) human primary preadipocytes (n= 4 independent experiments, paired t-test compared with Abd of same time point *p<0.05) (C).F=female; M=male
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4228784&req=5

Figure 1: Expression of HOTAIR exclusively in human gluteal sc adipose tissueMicroarray analysis identified HOTAIR as a gene expressed only in gluteal depot (A). HOTAIR expression by qPCR in WAT paired samples (n=35 subjects, paired t-test compared with Abd of same sex *p<0.001 **p<0.0001) (B) and in vitro during differentiation of paired abdominal (ABD) and gluteal (GLUT) human primary preadipocytes (n= 4 independent experiments, paired t-test compared with Abd of same time point *p<0.05) (C).F=female; M=male
Mentions: We identified HOTAIR as a long non-coding RNA expressed in gluteal but not abdominal sc adipose tissue in both sexes (Fig. 1A). These microarray results were verified using qPCR in the same group of subjects: HOTAIR was at the detection limit in abdominal sc adipose tissue samples (CT 37–40) and expressed in all gluteal adipose tissue samples (CT 29–36, Fig. 1B). HOTAIR gene expression was similar in males and females. HOTAIR expression was highly variable and enriched >10-fold in isolated gluteal adipocytes compared to gluteal adipose tissue, but was also expressed in gluteal stromal cells (tissue levels: 4.5±4.1 arbitrary units (AU), adipocytes: 58.8±23.7, stromal-vascular fraction 13.5±10.5 AU). In contrast, HOTAIR was at the limit of detection in both cell fractions of abdominal adipose tissue. To determine whether gluteal adipocytes expressed HOTAIR in a cell autonomous, depot-specific manner, we examined expression in primary cultures of preadipocytes from both depots. The depot difference in HOTAIR gene expression is maintained ex vivo throughout differentiation. Differentiation increased HOTAIR expression by 2-fold (Fig 1C).

Bottom Line: Physiological differences in gluteal compared with abdominal subcutaneous (sc) adipocyte functions are known but the molecular basis for depot differences in adipocyte function is poorly understood.The HOTAIR gene was stably transfected into primary cultured human abdominal sc preadipocytes using a lentivirus and effects on adipogenic differentiation were analyzed.The role of this lncRNA in determining the metabolic properties of gluteal compared with abdominal adipocytes merits further study.

View Article: PubMed Central - PubMed

Affiliation: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Sanford/Burnham Medical Research Institute, Orlando, Florida, USA; Diabetes and Obesity Research Center, Sanford/Burnham Medical Research Institute at Lake Nona, Orlando, Florida, USA.

Show MeSH
Related in: MedlinePlus