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Prepubertal exposure to genistein alleviates di-(2-ethylhexyl) phthalate induced testicular oxidative stress in adult rats.

Zhang LD, Li HC, Chong T, Gao M, Yin J, Fu DL, Deng Q, Wang ZM - Biomed Res Int (2014)

Bottom Line: Genistein (GEN) is one of the isoflavones ingredients exhibiting weak estrogenic and potentially antioxidative effects.Our results indicated that DEHP could significantly decrease sex organs weight, organ coefficient, and testicular antioxidative ability, which largely depended on the dose of DEHP.However, coadministration of GEN could partially alleviate DEHP-induced reproductive injuries via enhancement of testicular antioxidative enzymes activities, which indicates that GEN has protective effects on DEHP-induced male reproductive system damage after prepubertal exposure and GEN may have promising future in its curative antioxidative role for reproductive disorders caused by other environmental endocrine disruptors.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.

ABSTRACT
Di-(2-ethylhexyl) phthalate (DEHP) is the most widely used plastizer in the world and can suppress testosterone production via activation of oxidative stress. Genistein (GEN) is one of the isoflavones ingredients exhibiting weak estrogenic and potentially antioxidative effects. However, study on reproductive effects following prepubertal multiple endocrine disrupters exposure has been lacking. In this study, DEHP and GEN were administrated to prepubertal male Sprague-Dawley rats by gavage from postnatal day 22 (PND22) to PND35 with vehicle control, GEN at 50 mg/kg body weight (bw)/day (G), DEHP at 50, 150, 450 mg/kg bw/day (D50, D150, D450) and their mixture (G + D50, G + D150, G + D450). On PND90, general morphometry (body weight, AGD, organ weight, and organ coefficient), testicular redox state, and testicular histology were studied. Our results indicated that DEHP could significantly decrease sex organs weight, organ coefficient, and testicular antioxidative ability, which largely depended on the dose of DEHP. However, coadministration of GEN could partially alleviate DEHP-induced reproductive injuries via enhancement of testicular antioxidative enzymes activities, which indicates that GEN has protective effects on DEHP-induced male reproductive system damage after prepubertal exposure and GEN may have promising future in its curative antioxidative role for reproductive disorders caused by other environmental endocrine disruptors.

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Testicular redox state comparison between groups on PND90. ∗: significantly different from control at P < 0.05; #: significantly different from G at P < 0.05; and ■: significantly different from corresponding D at P < 0.05.
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fig2: Testicular redox state comparison between groups on PND90. ∗: significantly different from control at P < 0.05; #: significantly different from G at P < 0.05; and ■: significantly different from corresponding D at P < 0.05.

Mentions: Testicular redox state in all groups on PND90 was shown in Figure 2. Exposure to D50 and D150 as well as D450 resulted in significant reduction of testicular T-AOC, SOD activity, CAT, and GSH-PX levels as well as the ratio of GSH/GSSG (P < 0.05) while the combination of genistein with DEHP showed significant increase compared with corresponding single-DEHP exposure (P < 0.05), which indicates that genistein could partially enhance testicular antioxidative ability. In contrast, MDA level in D50, 150, and D450 increase significantly compared with control (P < 0.05), while the combination of genistein and DEHP showed significant decrease compared with corresponding single-DEHP exposure (P < 0.05), which may be largely due to the increased antioxidative capacity after genistein treatment.


Prepubertal exposure to genistein alleviates di-(2-ethylhexyl) phthalate induced testicular oxidative stress in adult rats.

Zhang LD, Li HC, Chong T, Gao M, Yin J, Fu DL, Deng Q, Wang ZM - Biomed Res Int (2014)

Testicular redox state comparison between groups on PND90. ∗: significantly different from control at P < 0.05; #: significantly different from G at P < 0.05; and ■: significantly different from corresponding D at P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4228721&req=5

fig2: Testicular redox state comparison between groups on PND90. ∗: significantly different from control at P < 0.05; #: significantly different from G at P < 0.05; and ■: significantly different from corresponding D at P < 0.05.
Mentions: Testicular redox state in all groups on PND90 was shown in Figure 2. Exposure to D50 and D150 as well as D450 resulted in significant reduction of testicular T-AOC, SOD activity, CAT, and GSH-PX levels as well as the ratio of GSH/GSSG (P < 0.05) while the combination of genistein with DEHP showed significant increase compared with corresponding single-DEHP exposure (P < 0.05), which indicates that genistein could partially enhance testicular antioxidative ability. In contrast, MDA level in D50, 150, and D450 increase significantly compared with control (P < 0.05), while the combination of genistein and DEHP showed significant decrease compared with corresponding single-DEHP exposure (P < 0.05), which may be largely due to the increased antioxidative capacity after genistein treatment.

Bottom Line: Genistein (GEN) is one of the isoflavones ingredients exhibiting weak estrogenic and potentially antioxidative effects.Our results indicated that DEHP could significantly decrease sex organs weight, organ coefficient, and testicular antioxidative ability, which largely depended on the dose of DEHP.However, coadministration of GEN could partially alleviate DEHP-induced reproductive injuries via enhancement of testicular antioxidative enzymes activities, which indicates that GEN has protective effects on DEHP-induced male reproductive system damage after prepubertal exposure and GEN may have promising future in its curative antioxidative role for reproductive disorders caused by other environmental endocrine disruptors.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.

ABSTRACT
Di-(2-ethylhexyl) phthalate (DEHP) is the most widely used plastizer in the world and can suppress testosterone production via activation of oxidative stress. Genistein (GEN) is one of the isoflavones ingredients exhibiting weak estrogenic and potentially antioxidative effects. However, study on reproductive effects following prepubertal multiple endocrine disrupters exposure has been lacking. In this study, DEHP and GEN were administrated to prepubertal male Sprague-Dawley rats by gavage from postnatal day 22 (PND22) to PND35 with vehicle control, GEN at 50 mg/kg body weight (bw)/day (G), DEHP at 50, 150, 450 mg/kg bw/day (D50, D150, D450) and their mixture (G + D50, G + D150, G + D450). On PND90, general morphometry (body weight, AGD, organ weight, and organ coefficient), testicular redox state, and testicular histology were studied. Our results indicated that DEHP could significantly decrease sex organs weight, organ coefficient, and testicular antioxidative ability, which largely depended on the dose of DEHP. However, coadministration of GEN could partially alleviate DEHP-induced reproductive injuries via enhancement of testicular antioxidative enzymes activities, which indicates that GEN has protective effects on DEHP-induced male reproductive system damage after prepubertal exposure and GEN may have promising future in its curative antioxidative role for reproductive disorders caused by other environmental endocrine disruptors.

Show MeSH
Related in: MedlinePlus