Limits...
Removal of lipopolysaccharide from protein solution using nanostructured porous supports bearing lipid membranes.

Wakita MA - Nanoscale Res Lett (2013)

Bottom Line: When human serum albumin solution, including 5 mg mL-1 of albumin and 5.6 ng mL-1 of lipopolysaccharide, was passed through a column packed with the resulting porous supports bearing lipid membranes assembled in nanoscale, lipopolysaccharide was removed to as low as a detection limit of 0.020 ng mL-1 with a quantitative recovery of protein.The difference above as well as difference from conventional adsorbents suggested that the selectivity was attributable to an interaction between the cationic lipid membranes of N-octadecylchitosan and lipopolysaccharide as well as protein.The porous supports bearing lipid membranes were stable in 0.5 M NaOH and 0.1 M HCl at ambient temperature.

View Article: PubMed Central - HTML - PubMed

Affiliation: Kurita Global Technology Center, Kurita Water Industries, Ltd,, 1-1 Kawada, Nogi-machi, Shimotsuga-gun, Tochigi 329-0105, Japan. masaaki.wakita@kurita.co.jp.

ABSTRACT
Polymeric lipid membranes of N-octadecylchitosan, which consists of 70 mol% of 2-(octadecylamino)-2-deoxy-d-glucopyranose, 17 mol% of 2-amino-2-deoxy-d-glucopyranose, and 13 mol% of 2-acetamido-2-deoxy-d-glucopyranose, were covalently immobilized to carboxylated porous supports composed of chitosan and used for the adsorption of pyrogenic lipopolysaccharide. When human serum albumin solution, including 5 mg mL-1 of albumin and 5.6 ng mL-1 of lipopolysaccharide, was passed through a column packed with the resulting porous supports bearing lipid membranes assembled in nanoscale, lipopolysaccharide was removed to as low as a detection limit of 0.020 ng mL-1 with a quantitative recovery of protein. On the other hand, in the case of directly N-octadecylated porous supports having cationic and hydrophobic ligands which are not assembled as lipid membranes, lipopolysaccharide could not be removed to the detection limit and protein recovery was lower than the porous supports bearing lipid membranes. The difference above as well as difference from conventional adsorbents suggested that the selectivity was attributable to an interaction between the cationic lipid membranes of N-octadecylchitosan and lipopolysaccharide as well as protein. The porous supports bearing lipid membranes were stable in 0.5 M NaOH and 0.1 M HCl at ambient temperature. Considering the confirmed excellent selectivity and chemical stability, their practical use as separation media in the pharmaceutical manufacturing can be expected.

No MeSH data available.


Related in: MedlinePlus

Preparation schemes of the porous supports bearing lipid membranes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4228324&req=5

Figure 2: Preparation schemes of the porous supports bearing lipid membranes.

Mentions: Preparation of porous supports bearing lipid membranes is described briefly with the conceptual scheme (Figure 2). Chitosan was simply N-alkylated by 1-bromooctadecane in N,N-dimethylacetamide to yield N-octadecylchitosan consisting 70 mol% of GlcNC18, 17 mol% of GlcN, and 13 mol% of GlcNAc. In DSC of N-octadecylchitosan, an endothermic peak was observed (Tc = 46°C) indicating a gel to liquid-crystalline phase transition. Dispersion liquid was prepared by suspending N-octadecylchitosan in water including hydrochloric acid and successive sonication. Electron microscopic observation of the dispersion liquid revealed the existence of unilamellar vesicles having diameters of 10 to 150 nm [12]. Carboxylated porous supports were prepared by N-succinylation of the cross-linked porous chitosan with succinic anhydride. Vesicular dispersion of N-octadecylchitosan was reacted with the carboxylated porous supports in the presence of WSC and HOSu to form amide bonds from primary amino groups of N-octadecylchitosan and carboxyl groups of the porous supports. The resulting materials were further reacted with N-acetylglucosamine to block the remaining carboxyl groups by amidation [10].


Removal of lipopolysaccharide from protein solution using nanostructured porous supports bearing lipid membranes.

Wakita MA - Nanoscale Res Lett (2013)

Preparation schemes of the porous supports bearing lipid membranes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4228324&req=5

Figure 2: Preparation schemes of the porous supports bearing lipid membranes.
Mentions: Preparation of porous supports bearing lipid membranes is described briefly with the conceptual scheme (Figure 2). Chitosan was simply N-alkylated by 1-bromooctadecane in N,N-dimethylacetamide to yield N-octadecylchitosan consisting 70 mol% of GlcNC18, 17 mol% of GlcN, and 13 mol% of GlcNAc. In DSC of N-octadecylchitosan, an endothermic peak was observed (Tc = 46°C) indicating a gel to liquid-crystalline phase transition. Dispersion liquid was prepared by suspending N-octadecylchitosan in water including hydrochloric acid and successive sonication. Electron microscopic observation of the dispersion liquid revealed the existence of unilamellar vesicles having diameters of 10 to 150 nm [12]. Carboxylated porous supports were prepared by N-succinylation of the cross-linked porous chitosan with succinic anhydride. Vesicular dispersion of N-octadecylchitosan was reacted with the carboxylated porous supports in the presence of WSC and HOSu to form amide bonds from primary amino groups of N-octadecylchitosan and carboxyl groups of the porous supports. The resulting materials were further reacted with N-acetylglucosamine to block the remaining carboxyl groups by amidation [10].

Bottom Line: When human serum albumin solution, including 5 mg mL-1 of albumin and 5.6 ng mL-1 of lipopolysaccharide, was passed through a column packed with the resulting porous supports bearing lipid membranes assembled in nanoscale, lipopolysaccharide was removed to as low as a detection limit of 0.020 ng mL-1 with a quantitative recovery of protein.The difference above as well as difference from conventional adsorbents suggested that the selectivity was attributable to an interaction between the cationic lipid membranes of N-octadecylchitosan and lipopolysaccharide as well as protein.The porous supports bearing lipid membranes were stable in 0.5 M NaOH and 0.1 M HCl at ambient temperature.

View Article: PubMed Central - HTML - PubMed

Affiliation: Kurita Global Technology Center, Kurita Water Industries, Ltd,, 1-1 Kawada, Nogi-machi, Shimotsuga-gun, Tochigi 329-0105, Japan. masaaki.wakita@kurita.co.jp.

ABSTRACT
Polymeric lipid membranes of N-octadecylchitosan, which consists of 70 mol% of 2-(octadecylamino)-2-deoxy-d-glucopyranose, 17 mol% of 2-amino-2-deoxy-d-glucopyranose, and 13 mol% of 2-acetamido-2-deoxy-d-glucopyranose, were covalently immobilized to carboxylated porous supports composed of chitosan and used for the adsorption of pyrogenic lipopolysaccharide. When human serum albumin solution, including 5 mg mL-1 of albumin and 5.6 ng mL-1 of lipopolysaccharide, was passed through a column packed with the resulting porous supports bearing lipid membranes assembled in nanoscale, lipopolysaccharide was removed to as low as a detection limit of 0.020 ng mL-1 with a quantitative recovery of protein. On the other hand, in the case of directly N-octadecylated porous supports having cationic and hydrophobic ligands which are not assembled as lipid membranes, lipopolysaccharide could not be removed to the detection limit and protein recovery was lower than the porous supports bearing lipid membranes. The difference above as well as difference from conventional adsorbents suggested that the selectivity was attributable to an interaction between the cationic lipid membranes of N-octadecylchitosan and lipopolysaccharide as well as protein. The porous supports bearing lipid membranes were stable in 0.5 M NaOH and 0.1 M HCl at ambient temperature. Considering the confirmed excellent selectivity and chemical stability, their practical use as separation media in the pharmaceutical manufacturing can be expected.

No MeSH data available.


Related in: MedlinePlus