Limits...
Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case.

Feng L, Xu C, Zeng X, Zhang H, Yang F, Li W, Tu Z, Li C, Hu L - Croat. Med. J. (2014)

Bottom Line: Conclusion.The three Y haplotype units matched three known perpetrators' genotypes.Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.

View Article: PubMed Central - PubMed

Affiliation: Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China, 13911162826@139.com.

ABSTRACT

Aim: To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler(®) kit. Consensus DNA profiles were generated from 5 replicate experiments. Results. Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained. Conclusion. The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.

Show MeSH

Related in: MedlinePlus

Mixed Y-STR profile of the vaginal swab obtained by the routine method.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4228287&req=5

Figure 2: Mixed Y-STR profile of the vaginal swab obtained by the routine method.

Mentions: The vaginal swab contained the victim’s epithelial cells and three perpetrators’ sperm cells. Routine preferential lysis DNA analysis yielded a mixed Y-STR profile generated from multiple contributors (Figure 2). Single sperm assay was performed. Ninety-four independent reactions were performed using Yfiler Kit. Two complete allelic profiles and 20 acceptable profiles (13-15 loci) were obtained. Forty-one reactions (44%) produced valid Y-STR profiles of more than nine loci. Twenty-five, ten, and six valid profiles were obtained respectively for the perpetrator A, B, and C (Table 2). According to Gill’s guidelines, we obtained three distinct Y haplotype units (Supplementary Tables 2-4(web extra material 2), Figure 3), which corresponded to three perpetrators’ genotypes. This result served as supportive evidence for the case.


Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case.

Feng L, Xu C, Zeng X, Zhang H, Yang F, Li W, Tu Z, Li C, Hu L - Croat. Med. J. (2014)

Mixed Y-STR profile of the vaginal swab obtained by the routine method.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4228287&req=5

Figure 2: Mixed Y-STR profile of the vaginal swab obtained by the routine method.
Mentions: The vaginal swab contained the victim’s epithelial cells and three perpetrators’ sperm cells. Routine preferential lysis DNA analysis yielded a mixed Y-STR profile generated from multiple contributors (Figure 2). Single sperm assay was performed. Ninety-four independent reactions were performed using Yfiler Kit. Two complete allelic profiles and 20 acceptable profiles (13-15 loci) were obtained. Forty-one reactions (44%) produced valid Y-STR profiles of more than nine loci. Twenty-five, ten, and six valid profiles were obtained respectively for the perpetrator A, B, and C (Table 2). According to Gill’s guidelines, we obtained three distinct Y haplotype units (Supplementary Tables 2-4(web extra material 2), Figure 3), which corresponded to three perpetrators’ genotypes. This result served as supportive evidence for the case.

Bottom Line: Conclusion.The three Y haplotype units matched three known perpetrators' genotypes.Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.

View Article: PubMed Central - PubMed

Affiliation: Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China, 13911162826@139.com.

ABSTRACT

Aim: To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler(®) kit. Consensus DNA profiles were generated from 5 replicate experiments. Results. Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained. Conclusion. The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.

Show MeSH
Related in: MedlinePlus