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Genetic variations of ND5 gene of mtDNA in populations of Anopheles sinensis (Diptera: Culicidae) malaria vector in China.

Makhawi AM, Liu XB, Yang SR, Liu QY - Parasit Vectors (2013)

Bottom Line: The results of the present study support population growth and expansion in the An. sinensis populations from China.The results indicate that the ND5 gene of mtDNA is highly polymorphic in An. sinensis and has moderate genetic variability in the populations of this mosquito in China.Demographic and spatial results support evidence of expansion in An. sinensis populations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Vector Biology and Control, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. liuqiyong@icdc.cn.

ABSTRACT

Background: Anopheles sinensis is a principal vector for Plasmodium vivax malaria in most parts of China. Understanding of genetic structure and genetic differentiation of the mosquito should contribute to the vector control and malaria elimination in China.

Methods: The present study investigated the genetic structure of An. sinensis populations using a 729 bp fragment of mtDNA ND5 among 10 populations collected from seven provinces in China.

Results: ND5 was polymorphic by single mutations within three groups of An. sinensis that were collected from 10 different geographic populations in China. Out of 140 specimens collected from 10 representative sites, 84 haplotypes and 71 variable positions were determined. The overall level of genetic differentiation of An. sinensis varied from low to moderate across China and with a FST range of 0.00065-0.341. Genealogy analysis clustered the populations of An. sinensis into three main clusters. Each cluster shared one main haplotype. Pairwise variations within populations were higher (68.68%) than among populations (31.32%) and with high fixation index (FST = 0.313). The results of the present study support population growth and expansion in the An. sinensis populations from China. Three clusters of An. sinensis populations were detected in this study with each displaying different proportion patterns over seven Chinese provinces. No correlation between genetic and geographic distance was detected in overall populations of An. sinensis (R2 = 0.058; P = 0.301).

Conclusions: The results indicate that the ND5 gene of mtDNA is highly polymorphic in An. sinensis and has moderate genetic variability in the populations of this mosquito in China. Demographic and spatial results support evidence of expansion in An. sinensis populations.

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Correlation between average FSTand geographic distance between collection sites for pairwise comparisons of An. sinensis populations.P = 0.301.
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Figure 3: Correlation between average FSTand geographic distance between collection sites for pairwise comparisons of An. sinensis populations.P = 0.301.

Mentions: Neutrality tests of Tajima's D revealed non-significant negative values in all individual populations of the three clusters and pooled samples (Table 5). However, negative, large and highly significant Fu's Fs values were observed (P < 0.001) in the three clustered populations and pooled samples (Table 5). Fu's Fs is considered to be more sensitive in detecting deviations from neutrality and thereby suggesting possible population expansion. The expansion may have been restricted to separate local areas that resulted in the non-significant negative Tajima's D values for clusters and all populations. The Mantel test results showed in Figure 3 supported the AMOVA results, that entire pooled populations lack of correlations between genetic (FST) and geographic distance (R2 = 0.058, P = 0.301).


Genetic variations of ND5 gene of mtDNA in populations of Anopheles sinensis (Diptera: Culicidae) malaria vector in China.

Makhawi AM, Liu XB, Yang SR, Liu QY - Parasit Vectors (2013)

Correlation between average FSTand geographic distance between collection sites for pairwise comparisons of An. sinensis populations.P = 0.301.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4228240&req=5

Figure 3: Correlation between average FSTand geographic distance between collection sites for pairwise comparisons of An. sinensis populations.P = 0.301.
Mentions: Neutrality tests of Tajima's D revealed non-significant negative values in all individual populations of the three clusters and pooled samples (Table 5). However, negative, large and highly significant Fu's Fs values were observed (P < 0.001) in the three clustered populations and pooled samples (Table 5). Fu's Fs is considered to be more sensitive in detecting deviations from neutrality and thereby suggesting possible population expansion. The expansion may have been restricted to separate local areas that resulted in the non-significant negative Tajima's D values for clusters and all populations. The Mantel test results showed in Figure 3 supported the AMOVA results, that entire pooled populations lack of correlations between genetic (FST) and geographic distance (R2 = 0.058, P = 0.301).

Bottom Line: The results of the present study support population growth and expansion in the An. sinensis populations from China.The results indicate that the ND5 gene of mtDNA is highly polymorphic in An. sinensis and has moderate genetic variability in the populations of this mosquito in China.Demographic and spatial results support evidence of expansion in An. sinensis populations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Vector Biology and Control, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. liuqiyong@icdc.cn.

ABSTRACT

Background: Anopheles sinensis is a principal vector for Plasmodium vivax malaria in most parts of China. Understanding of genetic structure and genetic differentiation of the mosquito should contribute to the vector control and malaria elimination in China.

Methods: The present study investigated the genetic structure of An. sinensis populations using a 729 bp fragment of mtDNA ND5 among 10 populations collected from seven provinces in China.

Results: ND5 was polymorphic by single mutations within three groups of An. sinensis that were collected from 10 different geographic populations in China. Out of 140 specimens collected from 10 representative sites, 84 haplotypes and 71 variable positions were determined. The overall level of genetic differentiation of An. sinensis varied from low to moderate across China and with a FST range of 0.00065-0.341. Genealogy analysis clustered the populations of An. sinensis into three main clusters. Each cluster shared one main haplotype. Pairwise variations within populations were higher (68.68%) than among populations (31.32%) and with high fixation index (FST = 0.313). The results of the present study support population growth and expansion in the An. sinensis populations from China. Three clusters of An. sinensis populations were detected in this study with each displaying different proportion patterns over seven Chinese provinces. No correlation between genetic and geographic distance was detected in overall populations of An. sinensis (R2 = 0.058; P = 0.301).

Conclusions: The results indicate that the ND5 gene of mtDNA is highly polymorphic in An. sinensis and has moderate genetic variability in the populations of this mosquito in China. Demographic and spatial results support evidence of expansion in An. sinensis populations.

Show MeSH
Related in: MedlinePlus