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Dendropanax morbifera Léveille extract facilitates cadmium excretion and prevents oxidative damage in the hippocampus by increasing antioxidant levels in cadmium-exposed rats.

Kim W, Kim DW, Yoo DY, Jung HY, Nam SM, Kim JW, Hong SM, Kim DW, Choi JH, Moon SM, Yoon YS, Hwang IK - BMC Complement Altern Med (2014)

Bottom Line: In addition, the effects of Cd and/or DMS on oxidative stress were assayed by measuring reactive oxygen species production, protein carbonyl modification, lipid peroxidation levels, and antioxidant levels in hippocampal homogenates.DMS treatment significantly reduced Cd content in the blood and kidneys, but not in the hippocampi.These results indicate that DMS facilitates cadmium excretion from the kidneys, reduces cadmium-induced oxidative stress in the hippocampus, and modulates SOD1, CAT, GPx, and glutathione-S-transferase activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea. vetmed2@snu.ac.kr.

ABSTRACT

Background: Dendropanax morbifera Léveille is used in herbal medicine as a cancer treatment. In this study, we investigated the effects of Dendropanax morbifera stem extract (DMS) on cadmium (Cd) excretion from the blood and kidney and brain tissues of rats exposed to cadmium, as well as the effects of DMS on oxidative stress and antioxidant levels in the hippocampus after Cd exposure.

Methods: Seven-week-old Sprague-Dawley rats were exposed to 2 mg/kg of cadmium by intragastric gavage and were orally administered 100 mg/kg of DMS for 4 weeks. Animals were sacrificed and Cd determination was performed using inductively coupled plasma mass spectrometry. In addition, the effects of Cd and/or DMS on oxidative stress were assayed by measuring reactive oxygen species production, protein carbonyl modification, lipid peroxidation levels, and antioxidant levels in hippocampal homogenates.

Results: Exposure to Cd significantly increased Cd content in the blood, kidneys, and hippocampi. DMS treatment significantly reduced Cd content in the blood and kidneys, but not in the hippocampi. Exposure to Cd significantly increased reactive oxygen species production, protein carbonyl modification, lipid peroxidation, total sulfhydryl content, reduced glutathione content, and glutathione reductase activity. In contrast, Cu, Zn-superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) activity in the hippocampus were significantly decreased after exposure to Cd, and administration of DMS significantly inhibited these Cd-induced changes.

Conclusion: These results indicate that DMS facilitates cadmium excretion from the kidneys, reduces cadmium-induced oxidative stress in the hippocampus, and modulates SOD1, CAT, GPx, and glutathione-S-transferase activities.

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Related in: MedlinePlus

Levels of intracellular reactive oxygen species production as determined by 2′,7′-dichlorofluorescein (DCF) levels (A), protein carbonyl levels (B), and malondialdehyde (MDA) levels (C) in the hippocampi of control, DMS-, Cd-, and Cd-DMS-treated rats. * Indicates a significant difference between the control and Cd groups (P <0.05); # indicates a significant difference between the Cd and Cd/DMS groups (P <0.05; n = 5-7 per group). DCF, protein carbonyl, and MDA levels are significantly higher in the Cd group and the administration of DMS to Cd-exposed rats leads to a decrease in DCF, protein carbonyl, and MDA levels. The data represent means ± standard error of the mean (SEM).
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Fig1: Levels of intracellular reactive oxygen species production as determined by 2′,7′-dichlorofluorescein (DCF) levels (A), protein carbonyl levels (B), and malondialdehyde (MDA) levels (C) in the hippocampi of control, DMS-, Cd-, and Cd-DMS-treated rats. * Indicates a significant difference between the control and Cd groups (P <0.05); # indicates a significant difference between the Cd and Cd/DMS groups (P <0.05; n = 5-7 per group). DCF, protein carbonyl, and MDA levels are significantly higher in the Cd group and the administration of DMS to Cd-exposed rats leads to a decrease in DCF, protein carbonyl, and MDA levels. The data represent means ± standard error of the mean (SEM).

Mentions: DCF fluorescence levels were similar in the control and DMS-treated groups. DCF fluorescence was significantly increased (1.71-fold) in the Cd-treated group in comparison with that of the control group. However, the MDA level of the Cd-DMS-treated group was significantly decreased to 74.4% of that of the Cd-treated group (Figure 1A).Figure 1


Dendropanax morbifera Léveille extract facilitates cadmium excretion and prevents oxidative damage in the hippocampus by increasing antioxidant levels in cadmium-exposed rats.

Kim W, Kim DW, Yoo DY, Jung HY, Nam SM, Kim JW, Hong SM, Kim DW, Choi JH, Moon SM, Yoon YS, Hwang IK - BMC Complement Altern Med (2014)

Levels of intracellular reactive oxygen species production as determined by 2′,7′-dichlorofluorescein (DCF) levels (A), protein carbonyl levels (B), and malondialdehyde (MDA) levels (C) in the hippocampi of control, DMS-, Cd-, and Cd-DMS-treated rats. * Indicates a significant difference between the control and Cd groups (P <0.05); # indicates a significant difference between the Cd and Cd/DMS groups (P <0.05; n = 5-7 per group). DCF, protein carbonyl, and MDA levels are significantly higher in the Cd group and the administration of DMS to Cd-exposed rats leads to a decrease in DCF, protein carbonyl, and MDA levels. The data represent means ± standard error of the mean (SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4228155&req=5

Fig1: Levels of intracellular reactive oxygen species production as determined by 2′,7′-dichlorofluorescein (DCF) levels (A), protein carbonyl levels (B), and malondialdehyde (MDA) levels (C) in the hippocampi of control, DMS-, Cd-, and Cd-DMS-treated rats. * Indicates a significant difference between the control and Cd groups (P <0.05); # indicates a significant difference between the Cd and Cd/DMS groups (P <0.05; n = 5-7 per group). DCF, protein carbonyl, and MDA levels are significantly higher in the Cd group and the administration of DMS to Cd-exposed rats leads to a decrease in DCF, protein carbonyl, and MDA levels. The data represent means ± standard error of the mean (SEM).
Mentions: DCF fluorescence levels were similar in the control and DMS-treated groups. DCF fluorescence was significantly increased (1.71-fold) in the Cd-treated group in comparison with that of the control group. However, the MDA level of the Cd-DMS-treated group was significantly decreased to 74.4% of that of the Cd-treated group (Figure 1A).Figure 1

Bottom Line: In addition, the effects of Cd and/or DMS on oxidative stress were assayed by measuring reactive oxygen species production, protein carbonyl modification, lipid peroxidation levels, and antioxidant levels in hippocampal homogenates.DMS treatment significantly reduced Cd content in the blood and kidneys, but not in the hippocampi.These results indicate that DMS facilitates cadmium excretion from the kidneys, reduces cadmium-induced oxidative stress in the hippocampus, and modulates SOD1, CAT, GPx, and glutathione-S-transferase activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea. vetmed2@snu.ac.kr.

ABSTRACT

Background: Dendropanax morbifera Léveille is used in herbal medicine as a cancer treatment. In this study, we investigated the effects of Dendropanax morbifera stem extract (DMS) on cadmium (Cd) excretion from the blood and kidney and brain tissues of rats exposed to cadmium, as well as the effects of DMS on oxidative stress and antioxidant levels in the hippocampus after Cd exposure.

Methods: Seven-week-old Sprague-Dawley rats were exposed to 2 mg/kg of cadmium by intragastric gavage and were orally administered 100 mg/kg of DMS for 4 weeks. Animals were sacrificed and Cd determination was performed using inductively coupled plasma mass spectrometry. In addition, the effects of Cd and/or DMS on oxidative stress were assayed by measuring reactive oxygen species production, protein carbonyl modification, lipid peroxidation levels, and antioxidant levels in hippocampal homogenates.

Results: Exposure to Cd significantly increased Cd content in the blood, kidneys, and hippocampi. DMS treatment significantly reduced Cd content in the blood and kidneys, but not in the hippocampi. Exposure to Cd significantly increased reactive oxygen species production, protein carbonyl modification, lipid peroxidation, total sulfhydryl content, reduced glutathione content, and glutathione reductase activity. In contrast, Cu, Zn-superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) activity in the hippocampus were significantly decreased after exposure to Cd, and administration of DMS significantly inhibited these Cd-induced changes.

Conclusion: These results indicate that DMS facilitates cadmium excretion from the kidneys, reduces cadmium-induced oxidative stress in the hippocampus, and modulates SOD1, CAT, GPx, and glutathione-S-transferase activities.

Show MeSH
Related in: MedlinePlus