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Laser-assisted zona pellucida thinning does not facilitate hatching and may disrupt the in vitro hatching process: a morphokinetic study in the mouse.

Schimmel T, Cohen J, Saunders H, Alikani M - Hum. Reprod. (2014)

Bottom Line: Overall treatment differences for continuous variables were analyzed by analysis of variance and pairwise comparisons using the Student t-test allowing for unequal variances, while for categorical data, a standard chi-squared test was utilized for all pairwise comparisons.Likewise, 53.6% of zona-thinned embryos had multiple breaches, always involving an area outside the thinned zone.Whether human embryos would behave the same way under similar circumstances is unknown.

View Article: PubMed Central - PubMed

Affiliation: Tyho-Galileo Research Laboratories, ART Institute of Washington, 3 Regent Street, Suite 301, Livingston, NJ 07039, USA.

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Calibration of zona opening (A–C) and thinning (D–F) in the mouse. Same number of pulses were used in each procedure. A calibration pulse was used visible at 4 o'clock in each instance. Experimental embryos were lased after calibration on each day. The calibration pulse was only used in spare embryos. Zona opening consisted of a set row of pulses on the outside of the zona pellucida (A) followed by a row of pulses carefully placed on the inside. The first of those pulses is shown in (B). The scale bar in (A) applies also to (B)–(F).
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DEU245F1: Calibration of zona opening (A–C) and thinning (D–F) in the mouse. Same number of pulses were used in each procedure. A calibration pulse was used visible at 4 o'clock in each instance. Experimental embryos were lased after calibration on each day. The calibration pulse was only used in spare embryos. Zona opening consisted of a set row of pulses on the outside of the zona pellucida (A) followed by a row of pulses carefully placed on the inside. The first of those pulses is shown in (B). The scale bar in (A) applies also to (B)–(F).

Mentions: The laser system included the Fertilase 1.48 μm laser, 100 mW, operated by Octax Eyeware digital interface (MTG, Bruckberg, Germany) positioned on an Olympus IX70 inverted microscope equipped with an Octax Adaptive Electronic Condenser (MTG) and Narishige micromanipulators (Narishige, East Meadow, NY, USA). The amount of total laser energy used was the same in all groups during each experiment. Before each experiment, the system was extensively calibrated and lasing was validated in spare embryos in order to standardize the diameter of the ablated area for each experiment. This standard was maintained throughout (Fig. 1).Figure 1


Laser-assisted zona pellucida thinning does not facilitate hatching and may disrupt the in vitro hatching process: a morphokinetic study in the mouse.

Schimmel T, Cohen J, Saunders H, Alikani M - Hum. Reprod. (2014)

Calibration of zona opening (A–C) and thinning (D–F) in the mouse. Same number of pulses were used in each procedure. A calibration pulse was used visible at 4 o'clock in each instance. Experimental embryos were lased after calibration on each day. The calibration pulse was only used in spare embryos. Zona opening consisted of a set row of pulses on the outside of the zona pellucida (A) followed by a row of pulses carefully placed on the inside. The first of those pulses is shown in (B). The scale bar in (A) applies also to (B)–(F).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4227580&req=5

DEU245F1: Calibration of zona opening (A–C) and thinning (D–F) in the mouse. Same number of pulses were used in each procedure. A calibration pulse was used visible at 4 o'clock in each instance. Experimental embryos were lased after calibration on each day. The calibration pulse was only used in spare embryos. Zona opening consisted of a set row of pulses on the outside of the zona pellucida (A) followed by a row of pulses carefully placed on the inside. The first of those pulses is shown in (B). The scale bar in (A) applies also to (B)–(F).
Mentions: The laser system included the Fertilase 1.48 μm laser, 100 mW, operated by Octax Eyeware digital interface (MTG, Bruckberg, Germany) positioned on an Olympus IX70 inverted microscope equipped with an Octax Adaptive Electronic Condenser (MTG) and Narishige micromanipulators (Narishige, East Meadow, NY, USA). The amount of total laser energy used was the same in all groups during each experiment. Before each experiment, the system was extensively calibrated and lasing was validated in spare embryos in order to standardize the diameter of the ablated area for each experiment. This standard was maintained throughout (Fig. 1).Figure 1

Bottom Line: Overall treatment differences for continuous variables were analyzed by analysis of variance and pairwise comparisons using the Student t-test allowing for unequal variances, while for categorical data, a standard chi-squared test was utilized for all pairwise comparisons.Likewise, 53.6% of zona-thinned embryos had multiple breaches, always involving an area outside the thinned zone.Whether human embryos would behave the same way under similar circumstances is unknown.

View Article: PubMed Central - PubMed

Affiliation: Tyho-Galileo Research Laboratories, ART Institute of Washington, 3 Regent Street, Suite 301, Livingston, NJ 07039, USA.

Show MeSH
Related in: MedlinePlus