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Lactose as a "Trojan horse" for quantum dot cell transport.

Benito-Alifonso D, Tremel S, Hou B, Lockyear H, Mantell J, Fermin DJ, Verkade P, Berry M, Galan MC - Angew. Chem. Int. Ed. Engl. (2013)

Bottom Line: A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells.We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization.Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK).

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Synthesis of a 1:1 mixture of lactose/mannose-QDs 20 b and lactose/maltotriose-QDs 21 b and confocal microscopy images after 2 h incubation with AS cells: A) 20 b localized in early endosomes and B) 21 b internalization in the Golgi. QD shown in green, organelle tracker in red, and overlap in yellow.
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sch02: Synthesis of a 1:1 mixture of lactose/mannose-QDs 20 b and lactose/maltotriose-QDs 21 b and confocal microscopy images after 2 h incubation with AS cells: A) 20 b localized in early endosomes and B) 21 b internalization in the Golgi. QD shown in green, organelle tracker in red, and overlap in yellow.


Lactose as a "Trojan horse" for quantum dot cell transport.

Benito-Alifonso D, Tremel S, Hou B, Lockyear H, Mantell J, Fermin DJ, Verkade P, Berry M, Galan MC - Angew. Chem. Int. Ed. Engl. (2013)

Synthesis of a 1:1 mixture of lactose/mannose-QDs 20 b and lactose/maltotriose-QDs 21 b and confocal microscopy images after 2 h incubation with AS cells: A) 20 b localized in early endosomes and B) 21 b internalization in the Golgi. QD shown in green, organelle tracker in red, and overlap in yellow.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4227560&req=5

sch02: Synthesis of a 1:1 mixture of lactose/mannose-QDs 20 b and lactose/maltotriose-QDs 21 b and confocal microscopy images after 2 h incubation with AS cells: A) 20 b localized in early endosomes and B) 21 b internalization in the Golgi. QD shown in green, organelle tracker in red, and overlap in yellow.
Bottom Line: A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells.We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization.Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK).

Show MeSH
Related in: MedlinePlus