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Lactose as a "Trojan horse" for quantum dot cell transport.

Benito-Alifonso D, Tremel S, Hou B, Lockyear H, Mantell J, Fermin DJ, Verkade P, Berry M, Galan MC - Angew. Chem. Int. Ed. Engl. (2013)

Bottom Line: A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells.We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization.Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK).

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Representative confocal microscopy images showing internalization after 2 h incubation of: A) galactose-QD 14 b in the Golgi of HeLa cells; B) 14 b in the Lysosomes of AS cells; C) lactose-QD 17 b in the Golgi of HeLa cells; D) 17 b in the ER of AS cells. QD shown in green, organelle tracker in red, and overlap in yellow. For a full set of images and R values, see the Supporting Information.
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fig03: Representative confocal microscopy images showing internalization after 2 h incubation of: A) galactose-QD 14 b in the Golgi of HeLa cells; B) 14 b in the Lysosomes of AS cells; C) lactose-QD 17 b in the Golgi of HeLa cells; D) 17 b in the ER of AS cells. QD shown in green, organelle tracker in red, and overlap in yellow. For a full set of images and R values, see the Supporting Information.

Mentions: We then studied the effect that glycan type has on cell uptake. 60 % coated QD samples 14 b–19 b were incubated in serum-containing medium (see the Supporting Information for details) with both cell lines at 37 °C for 2 and 24 h, at which time intracellular glycan-coated QDs were visualized by confocal microscopy (Figure 3). Internalization within cell organelles was determined by calculating the Manders overlap coefficient (R)15 for each organelle marker and glycan-coated QD (Figure S6).


Lactose as a "Trojan horse" for quantum dot cell transport.

Benito-Alifonso D, Tremel S, Hou B, Lockyear H, Mantell J, Fermin DJ, Verkade P, Berry M, Galan MC - Angew. Chem. Int. Ed. Engl. (2013)

Representative confocal microscopy images showing internalization after 2 h incubation of: A) galactose-QD 14 b in the Golgi of HeLa cells; B) 14 b in the Lysosomes of AS cells; C) lactose-QD 17 b in the Golgi of HeLa cells; D) 17 b in the ER of AS cells. QD shown in green, organelle tracker in red, and overlap in yellow. For a full set of images and R values, see the Supporting Information.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4227560&req=5

fig03: Representative confocal microscopy images showing internalization after 2 h incubation of: A) galactose-QD 14 b in the Golgi of HeLa cells; B) 14 b in the Lysosomes of AS cells; C) lactose-QD 17 b in the Golgi of HeLa cells; D) 17 b in the ER of AS cells. QD shown in green, organelle tracker in red, and overlap in yellow. For a full set of images and R values, see the Supporting Information.
Mentions: We then studied the effect that glycan type has on cell uptake. 60 % coated QD samples 14 b–19 b were incubated in serum-containing medium (see the Supporting Information for details) with both cell lines at 37 °C for 2 and 24 h, at which time intracellular glycan-coated QDs were visualized by confocal microscopy (Figure 3). Internalization within cell organelles was determined by calculating the Manders overlap coefficient (R)15 for each organelle marker and glycan-coated QD (Figure S6).

Bottom Line: A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells.We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization.Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK).

Show MeSH
Related in: MedlinePlus