Lactose as a "Trojan horse" for quantum dot cell transport.
Bottom Line: A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells.We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization.Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.
Affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK).Show MeSH
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Mentions: We then studied the effect that glycan type has on cell uptake. 60 % coated QD samples 14 b–19 b were incubated in serum-containing medium (see the Supporting Information for details) with both cell lines at 37 °C for 2 and 24 h, at which time intracellular glycan-coated QDs were visualized by confocal microscopy (Figure 3). Internalization within cell organelles was determined by calculating the Manders overlap coefficient (R)15 for each organelle marker and glycan-coated QD (Figure S6).
Affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK).