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Lactose as a "Trojan horse" for quantum dot cell transport.

Benito-Alifonso D, Tremel S, Hou B, Lockyear H, Mantell J, Fermin DJ, Verkade P, Berry M, Galan MC - Angew. Chem. Int. Ed. Engl. (2013)

Bottom Line: A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells.We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization.Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK).

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Effects of QDs (5 d, 17 a–c) with different glycan densities on reductive metabolism in HeLa.
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fig02: Effects of QDs (5 d, 17 a–c) with different glycan densities on reductive metabolism in HeLa.

Mentions: Two cell lines, HeLa (human cervical cancer cells) and Araki Sasaki (AS, SV40-immortalized human corneal epithelium14) were chosen for the study. Preliminary toxicity and cell viability studies (Figure 2; see also Figures S4 and S5) were performed with dextran- (13) and lactose-coated (17 a,b,c) QDs at surface densities 100 %, 60 %, 40 %, and 0 % coated neutral-QDs 5 d. It was found that the treatment of cells with 5 d decreased metabolic activity compared to untreated cultures. The effects of 60 % Lactose QDs 17 b on cell metabolism and proliferation were not different from untreated control groups after a 24 h exposure to 17 b, suggesting that the optimum sugar density for cell uptake is found at 60 % glycan coating.


Lactose as a "Trojan horse" for quantum dot cell transport.

Benito-Alifonso D, Tremel S, Hou B, Lockyear H, Mantell J, Fermin DJ, Verkade P, Berry M, Galan MC - Angew. Chem. Int. Ed. Engl. (2013)

Effects of QDs (5 d, 17 a–c) with different glycan densities on reductive metabolism in HeLa.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4227560&req=5

fig02: Effects of QDs (5 d, 17 a–c) with different glycan densities on reductive metabolism in HeLa.
Mentions: Two cell lines, HeLa (human cervical cancer cells) and Araki Sasaki (AS, SV40-immortalized human corneal epithelium14) were chosen for the study. Preliminary toxicity and cell viability studies (Figure 2; see also Figures S4 and S5) were performed with dextran- (13) and lactose-coated (17 a,b,c) QDs at surface densities 100 %, 60 %, 40 %, and 0 % coated neutral-QDs 5 d. It was found that the treatment of cells with 5 d decreased metabolic activity compared to untreated cultures. The effects of 60 % Lactose QDs 17 b on cell metabolism and proliferation were not different from untreated control groups after a 24 h exposure to 17 b, suggesting that the optimum sugar density for cell uptake is found at 60 % glycan coating.

Bottom Line: A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells.We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization.Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK).

Show MeSH
Related in: MedlinePlus