Limits...
Cucurbitacin B inhibits proliferation and induces apoptosis via STAT3 pathway inhibition in A549 lung cancer cells.

Zhang M, Bian ZG, Zhang Y, Wang JH, Kan L, Wang X, Niu HY, He P - Mol Med Rep (2014)

Bottom Line: The present study was conducted to investigate whether cucurbitacin B (CuB), one of the most potent and widely used cucurbitacins, inhibits proliferation and induces apoptosis in the A549 lung cancer cell line.Apoptosis induced by CuB was shown to be associated with cytochrome c release, B-cell lymphoma 2 downregulation and signal transducer and activator of transcription 3 pathway inhibition.CuB may prove to be a useful approach for the chemotherapy of lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.

ABSTRACT
Natural products are a great source of cancer chemotherapeutic agents. The present study was conducted to investigate whether cucurbitacin B (CuB), one of the most potent and widely used cucurbitacins, inhibits proliferation and induces apoptosis in the A549 lung cancer cell line. Furthermore, CuB induced apoptosis of A549 cells in a -concentration-dependent manner, as determined by fluorescence microscopy, flow cytometry and transmission electron microscopy. The present study also demonstrated that CuB dose-dependently inhibited lung cancer cell proliferation, with cell cycle inhibition and cyclin B1 downregulation. Apoptosis induced by CuB was shown to be associated with cytochrome c release, B-cell lymphoma 2 downregulation and signal transducer and activator of transcription 3 pathway inhibition. CuB may prove to be a useful approach for the chemotherapy of lung cancer.

Show MeSH

Related in: MedlinePlus

Flow cytometric quantification of apoptotic cells. (A) CuB-induced apoptosis in A549 cells as assayed by Annexin V/PI staining. A549 cells were treated with CuB (0, 0.1 and 1.0 μmol/l) for 24 h. The cells were then harvested and stained with Annexin V/PI prior to flow cytometric analysis of apoptosis. (B) Quantification of apoptotic cells from A in a bar chart. *P<0.05 vs. the control group; **P<0.01 vs. the control group. CuB, cucurbitacin B; PI, propium iodide; FITC, fluorescein isothiocyanate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4227420&req=5

f4-mmr-10-06-2905: Flow cytometric quantification of apoptotic cells. (A) CuB-induced apoptosis in A549 cells as assayed by Annexin V/PI staining. A549 cells were treated with CuB (0, 0.1 and 1.0 μmol/l) for 24 h. The cells were then harvested and stained with Annexin V/PI prior to flow cytometric analysis of apoptosis. (B) Quantification of apoptotic cells from A in a bar chart. *P<0.05 vs. the control group; **P<0.01 vs. the control group. CuB, cucurbitacin B; PI, propium iodide; FITC, fluorescein isothiocyanate.

Mentions: AnnexinV/PI analysis was employed to examine the effect of CuB on apoptosis in A549 cells. It was identified that CuB induced apoptosis of A549 cells evidently. The percentages of early and late apoptotic cells were significantly increased compared with the control group. The proportion of apoptotic cells in the treated cells was increased in a dose-dependent manner (Fig. 4).


Cucurbitacin B inhibits proliferation and induces apoptosis via STAT3 pathway inhibition in A549 lung cancer cells.

Zhang M, Bian ZG, Zhang Y, Wang JH, Kan L, Wang X, Niu HY, He P - Mol Med Rep (2014)

Flow cytometric quantification of apoptotic cells. (A) CuB-induced apoptosis in A549 cells as assayed by Annexin V/PI staining. A549 cells were treated with CuB (0, 0.1 and 1.0 μmol/l) for 24 h. The cells were then harvested and stained with Annexin V/PI prior to flow cytometric analysis of apoptosis. (B) Quantification of apoptotic cells from A in a bar chart. *P<0.05 vs. the control group; **P<0.01 vs. the control group. CuB, cucurbitacin B; PI, propium iodide; FITC, fluorescein isothiocyanate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4227420&req=5

f4-mmr-10-06-2905: Flow cytometric quantification of apoptotic cells. (A) CuB-induced apoptosis in A549 cells as assayed by Annexin V/PI staining. A549 cells were treated with CuB (0, 0.1 and 1.0 μmol/l) for 24 h. The cells were then harvested and stained with Annexin V/PI prior to flow cytometric analysis of apoptosis. (B) Quantification of apoptotic cells from A in a bar chart. *P<0.05 vs. the control group; **P<0.01 vs. the control group. CuB, cucurbitacin B; PI, propium iodide; FITC, fluorescein isothiocyanate.
Mentions: AnnexinV/PI analysis was employed to examine the effect of CuB on apoptosis in A549 cells. It was identified that CuB induced apoptosis of A549 cells evidently. The percentages of early and late apoptotic cells were significantly increased compared with the control group. The proportion of apoptotic cells in the treated cells was increased in a dose-dependent manner (Fig. 4).

Bottom Line: The present study was conducted to investigate whether cucurbitacin B (CuB), one of the most potent and widely used cucurbitacins, inhibits proliferation and induces apoptosis in the A549 lung cancer cell line.Apoptosis induced by CuB was shown to be associated with cytochrome c release, B-cell lymphoma 2 downregulation and signal transducer and activator of transcription 3 pathway inhibition.CuB may prove to be a useful approach for the chemotherapy of lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.

ABSTRACT
Natural products are a great source of cancer chemotherapeutic agents. The present study was conducted to investigate whether cucurbitacin B (CuB), one of the most potent and widely used cucurbitacins, inhibits proliferation and induces apoptosis in the A549 lung cancer cell line. Furthermore, CuB induced apoptosis of A549 cells in a -concentration-dependent manner, as determined by fluorescence microscopy, flow cytometry and transmission electron microscopy. The present study also demonstrated that CuB dose-dependently inhibited lung cancer cell proliferation, with cell cycle inhibition and cyclin B1 downregulation. Apoptosis induced by CuB was shown to be associated with cytochrome c release, B-cell lymphoma 2 downregulation and signal transducer and activator of transcription 3 pathway inhibition. CuB may prove to be a useful approach for the chemotherapy of lung cancer.

Show MeSH
Related in: MedlinePlus