Limits...
Genetic profiling of the Plasmodium falciparum population using antigenic molecular markers.

Gupta P, Singh R, Khan H, Raza A, Yadavendu V, Bhatt RM, Singh V - ScientificWorldJournal (2014)

Bottom Line: The molecular genotyping of the parasite populations by merozoite surface protein (msp1 and msp2) and glutamate-rich protein (glurp) genes identifies the existing parasite population in the regions which help in understanding the molecular mechanisms involved in the parasite's drive for survival.This study reveals the genetic profile of the parasite population in selected regions across the country with varying degree of endemicity among them.We also report the prevalence of Pfcrt mutations in this parasite population to evaluate the pattern of drug resistance development in them.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Malaria Research (ICMR), Sector 8, Dwarka, New Delhi 110077, India.

ABSTRACT
About 50% of malaria infections in India are attributed to Plasmodium falciparum but relatively little is known about the genetic structure of the parasite populations. The molecular genotyping of the parasite populations by merozoite surface protein (msp1 and msp2) and glutamate-rich protein (glurp) genes identifies the existing parasite population in the regions which help in understanding the molecular mechanisms involved in the parasite's drive for survival. This study reveals the genetic profile of the parasite population in selected regions across the country with varying degree of endemicity among them. We also report the prevalence of Pfcrt mutations in this parasite population to evaluate the pattern of drug resistance development in them.

Show MeSH

Related in: MedlinePlus

Genetic diversity of P. falciparum by msp1, msp2, and glurp genes. PCR amplification was represented by groups where PCR product size differed by 20 bp. (a) Genetic diversity by msp1 gene. Blue bars denote the K1 allelic family, size ranging from 180 to 300 bp; red bars denote the MAD20 allelic family, size ranging from 100 to 220 bp; and green bars denote the RO33 allelic family, size ranging from 100 to 200 bp. (b) Genetic diversity by msp2 gene. Blue bars denote the FC27 allelic family, size ranging from 280 to 800 bp and the red bars denote the IC3D7 allelic family, size ranging from 400 to 1150 bp. (c) Genetic diversity by glurp gene. Blue bars denote the RII repeat region of glurp gene, size ranging from 700 to 1200 bp.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4227404&req=5

fig3: Genetic diversity of P. falciparum by msp1, msp2, and glurp genes. PCR amplification was represented by groups where PCR product size differed by 20 bp. (a) Genetic diversity by msp1 gene. Blue bars denote the K1 allelic family, size ranging from 180 to 300 bp; red bars denote the MAD20 allelic family, size ranging from 100 to 220 bp; and green bars denote the RO33 allelic family, size ranging from 100 to 200 bp. (b) Genetic diversity by msp2 gene. Blue bars denote the FC27 allelic family, size ranging from 280 to 800 bp and the red bars denote the IC3D7 allelic family, size ranging from 400 to 1150 bp. (c) Genetic diversity by glurp gene. Blue bars denote the RII repeat region of glurp gene, size ranging from 700 to 1200 bp.

Mentions: After the PCR assay, the classification of the alleles was done according to the number and size of fragments and the allelic family (Figure 2). The msp1 gene block-1 amplification for K1 allelic family was positive in 29 samples (36.25%) with five different allelic sizes ranging from 180 to 300 bp among which 200 bp allelic fragment was predominant. The other family MAD20 in msp1 was found in 32 samples (40.0%) depicting seven different allelic sizes within 100–300 bp and 200 bp was found to be the predominant allele size in this group. The RO33 family was detected in 17 samples (21.25%) having four distinct alleles with fragment sizes of 100–200 bp and its predominant allele was found to be 200 bp fragment (Figure 3(a)). The msp2 amplification for FC27 family was positive in 37 isolates (46.25%) having seven different allele types ranging from 280 to 800 bp predominated by 300 bp fragment size. The IC3D7 allelic family was amplified in 45 isolates (56.25%) demarcated by nine different alleles spanning between 400 and 1150 bp fragments where 500 bp fragment was in majority (Figure 3(b)). For glurp 51 samples (63.75%) were found to be positive for RII repeat region producing eight different sizes ranging between 700 and 1200 bp, among which 900 bp allele was found to be predominant (25.0%) (Figure 3(c)). The number of genotypes for each marker is shown in Tables 2 and 3.


Genetic profiling of the Plasmodium falciparum population using antigenic molecular markers.

Gupta P, Singh R, Khan H, Raza A, Yadavendu V, Bhatt RM, Singh V - ScientificWorldJournal (2014)

Genetic diversity of P. falciparum by msp1, msp2, and glurp genes. PCR amplification was represented by groups where PCR product size differed by 20 bp. (a) Genetic diversity by msp1 gene. Blue bars denote the K1 allelic family, size ranging from 180 to 300 bp; red bars denote the MAD20 allelic family, size ranging from 100 to 220 bp; and green bars denote the RO33 allelic family, size ranging from 100 to 200 bp. (b) Genetic diversity by msp2 gene. Blue bars denote the FC27 allelic family, size ranging from 280 to 800 bp and the red bars denote the IC3D7 allelic family, size ranging from 400 to 1150 bp. (c) Genetic diversity by glurp gene. Blue bars denote the RII repeat region of glurp gene, size ranging from 700 to 1200 bp.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4227404&req=5

fig3: Genetic diversity of P. falciparum by msp1, msp2, and glurp genes. PCR amplification was represented by groups where PCR product size differed by 20 bp. (a) Genetic diversity by msp1 gene. Blue bars denote the K1 allelic family, size ranging from 180 to 300 bp; red bars denote the MAD20 allelic family, size ranging from 100 to 220 bp; and green bars denote the RO33 allelic family, size ranging from 100 to 200 bp. (b) Genetic diversity by msp2 gene. Blue bars denote the FC27 allelic family, size ranging from 280 to 800 bp and the red bars denote the IC3D7 allelic family, size ranging from 400 to 1150 bp. (c) Genetic diversity by glurp gene. Blue bars denote the RII repeat region of glurp gene, size ranging from 700 to 1200 bp.
Mentions: After the PCR assay, the classification of the alleles was done according to the number and size of fragments and the allelic family (Figure 2). The msp1 gene block-1 amplification for K1 allelic family was positive in 29 samples (36.25%) with five different allelic sizes ranging from 180 to 300 bp among which 200 bp allelic fragment was predominant. The other family MAD20 in msp1 was found in 32 samples (40.0%) depicting seven different allelic sizes within 100–300 bp and 200 bp was found to be the predominant allele size in this group. The RO33 family was detected in 17 samples (21.25%) having four distinct alleles with fragment sizes of 100–200 bp and its predominant allele was found to be 200 bp fragment (Figure 3(a)). The msp2 amplification for FC27 family was positive in 37 isolates (46.25%) having seven different allele types ranging from 280 to 800 bp predominated by 300 bp fragment size. The IC3D7 allelic family was amplified in 45 isolates (56.25%) demarcated by nine different alleles spanning between 400 and 1150 bp fragments where 500 bp fragment was in majority (Figure 3(b)). For glurp 51 samples (63.75%) were found to be positive for RII repeat region producing eight different sizes ranging between 700 and 1200 bp, among which 900 bp allele was found to be predominant (25.0%) (Figure 3(c)). The number of genotypes for each marker is shown in Tables 2 and 3.

Bottom Line: The molecular genotyping of the parasite populations by merozoite surface protein (msp1 and msp2) and glutamate-rich protein (glurp) genes identifies the existing parasite population in the regions which help in understanding the molecular mechanisms involved in the parasite's drive for survival.This study reveals the genetic profile of the parasite population in selected regions across the country with varying degree of endemicity among them.We also report the prevalence of Pfcrt mutations in this parasite population to evaluate the pattern of drug resistance development in them.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Malaria Research (ICMR), Sector 8, Dwarka, New Delhi 110077, India.

ABSTRACT
About 50% of malaria infections in India are attributed to Plasmodium falciparum but relatively little is known about the genetic structure of the parasite populations. The molecular genotyping of the parasite populations by merozoite surface protein (msp1 and msp2) and glutamate-rich protein (glurp) genes identifies the existing parasite population in the regions which help in understanding the molecular mechanisms involved in the parasite's drive for survival. This study reveals the genetic profile of the parasite population in selected regions across the country with varying degree of endemicity among them. We also report the prevalence of Pfcrt mutations in this parasite population to evaluate the pattern of drug resistance development in them.

Show MeSH
Related in: MedlinePlus